In Search of New Disease Models in the Mouse Using ENU Mutagenesis

Hough, Tertius; Aj, Hunter; Hardisty, Rachel E.

doi:10.1007/978-3-662-04667-8_7

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Since then, the ENU mutagenesis approach has also been utilized for the identification of gene mutations for abnormal phenotypes related to oncogenesis (Schindewolf et al, 2000) and malformation of eyes (Baird et al, 2002;Thaung et al, 2002), among other processes.…”

Section: (2) Large-scale Screening Of Enu Mutantsmentioning

confidence: 99%

“…The isolation of a circadian rhythm mutant by the ENU screening method, and the cloning of the key gene (King and Takahashi, 2000), were pivotal in demonstrating the unique advantages of this technique. Since then, the ENU mutagenesis approach has also been utilized for the identification of gene mutations for abnormal phenotypes related to oncogenesis (Schindewolf et al, 2000) and malformation of eyes (Baird et al, 2002;Thaung et al, 2002), among other processes.…”

Section: (2) Large-scale Screening Of Enu Mutantsmentioning

confidence: 99%

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ENU Large-scale Mutagenesis and Quantitative Trait Linkage (QTL) Analysis in Mice: Novel Technologies for Searching Polygenetic Determinants of Craniofacial Abnormalities

Nishimura

Drake

Lusis

et al. 2003

Critical Reviews in Oral Biology & Medicine

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Discrepancies in size and shape of the jaws are the underlying etiology in many orthodontic and orthognathic surgery patients. Genetic factors combined with environmental interactions have been postulated to play a causal or contributory role in these craniofacial abnormalities. Along with the soon-to-be-available complete human and mouse genomic sequence data, mouse mutants have become a valuable tool in the functional mapping of genes involved in the development of human maxillofacial dysmorphologies. We review two powerful methods in such efforts: N-ethyl-N-nitrosourea (ENU) large-scale mutagenesis and quantitative trait linkage (QTL) analysis. The former aims at producing a plethora of novel variants of particular trait(s), and ultimately mapping the point mutations responsible for the appearance of these new traits. In contrast, the latter applies intensive breeding and mapping techniques to identify multiple loci (and, subsequently, genes) contributing to the phenotypic difference between the tested strains. A prerequisite for either approach to studying variations in the traits of interest is the application of effective mouse cephalometric phenotype analysis and rapid DNA mapping techniques. These approaches will produce a wealth of new data on critical genes that influence the size and shape of the human face.

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Section: (2) Large-scale Screening Of Enu Mutantsmentioning

confidence: 99%

Section: (2) Large-scale Screening Of Enu Mutantsmentioning

confidence: 99%

ENU Large-scale Mutagenesis and Quantitative Trait Linkage (QTL) Analysis in Mice: Novel Technologies for Searching Polygenetic Determinants of Craniofacial Abnormalities

Nishimura

Drake

Lusis

et al. 2003

Critical Reviews in Oral Biology & Medicine

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Oxygen-induced retinopathy in mice with retinal photoreceptor cell degeneration

Zhang

2014

Life Sciences

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ENU mutagenesis in the mouse: Application to human genetic disease

Nolan

Hugill

Cox

2002

Briefings in Functional Genomics and Proteomics

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Genetic approaches in model organisms provide a powerful means by which to examine the biological basis of human diseases as well as the physiological processes that are affected by them. Although not without its drawbacks, the mouse has become the mammalian species of choice in studying the molecular basis of disease. Targeted mutagenesis approaches in the mouse have led to dramatic increases in our understanding of human disease processes. As a complement to these gene-driven studies, three developments have led to the reassessment of a phenotype-driven approach in the mouse--the accumulation of information that has emerged from human and mouse genome sequencing projects, the use of high-efficiency point mutagens such as N-ethyl-N-nitrosourea (ENU) and the application of systematic hierarchical screening protocols for the mouse. In this paper, progress with existing phenotypic screening programmes is discussed and opportunities for the development of new mouse disease models are presented.

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In Search of New Disease Models in the Mouse Using ENU Mutagenesis

Cited by 3 publications

References 33 publications

ENU Large-scale Mutagenesis and Quantitative Trait Linkage (QTL) Analysis in Mice: Novel Technologies for Searching Polygenetic Determinants of Craniofacial Abnormalities

ENU Large-scale Mutagenesis and Quantitative Trait Linkage (QTL) Analysis in Mice: Novel Technologies for Searching Polygenetic Determinants of Craniofacial Abnormalities

Oxygen-induced retinopathy in mice with retinal photoreceptor cell degeneration

ENU mutagenesis in the mouse: Application to human genetic disease

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