In Pursuit of the Elimination of Corneal Blindness: Is Establishing Eye Banks and Training Surgeons Enough?

Jeng, Bennie H.; Ahmad, Samera

doi:10.1016/j.ophtha.2020.06.042

Cited by 28 publications

(25 citation statements)

References 11 publications

(12 reference statements)

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“…Corneal blindness is the fourth leading cause of blindness worldwide (Jeng and Ahmad, 2021); however, nearly 80% of all cases are avoidable and reversible (Jeng and Ahmad, 2021). Thus, treating corneal diseases such as trauma or keratoplasty represents an urgent or emergent care issue for ophthalmologists.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Human Nerve Growth Factor (Cenegermin)–Driven Corneal Wound Healing Process: An Evidence-Based Analysis

et al. 2022

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Purpose: To evaluate anterior segment optical coherence tomography (AS-OCT) to detect the wound healing process as per monitoring the effectiveness of cenegermin to treat moderate to severe neurotrophic keratoplasty.Methods: A retrospective chart review was realized to identify patients treated with cenegermin at the Clinica Oculistica, University of Genoa, Italy. All patients underwent careful examinations at baseline and follow-up visits. AS-OCT scans centered on the minimum corneal thickness (CT) area were always performed. We compared findings of AS-OCT with the findings from the slit-lamp examination. A linear regression analysis was used to evaluate factors associated with corneal healing. A further analysis, including a control group treated with 50% autologous serum (AS), was done to investigate and compare the efficacy of cenegermin.Results: Data from 16 eyes were studied. The average patients' age was 60.9 ± 21.1 years; five (31.2%) eyes experienced persistent epithelial defect and 11 (68.8%) eyes had neurotrophic corneal ulcer. The average reepithelialization time was 3.9 ± 0.5 weeks in the cenegermin group versus 5.9 ± 1.9 weeks in the AS group (p < 0.01). The AS‐OCT scans revealed an average CT at the thinnest point of 276.3 ± 74.1 μm before treatment with an average increase of 176.5 ± 60.3 μm at the end of the cenegermin treatment (B = −0.15; p = 0.035). The AS-OCT percentage increase in corneal thickness between the two groups was statistically significant (p < 0.02).Conclusion: Understanding the cascade of events involved in the nerve growth factor–driven corneal wound healing process is clinically meaningful for the clinician. AS-OCT is an effective tool for systematic anterior segment imaging, allowing the detailed detection of the front-to-back layered corneal structure for quantitative analysis and monitoring of the healing process.

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Section: Introductionmentioning

confidence: 99%

Recombinant Human Nerve Growth Factor (Cenegermin)–Driven Corneal Wound Healing Process: An Evidence-Based Analysis

et al. 2022

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“…After that, corneoscleral rims was digested in 10 mg mL −1 Dispase II (Roche, Indianapolis, USA) in a supplemented hormonal epithelial medium (SHEM) for 2 h. After being digested at 37 °C for 20 min, limbal epithelial sheets were obtained and then incubated in Trypsin/EDTA at 37 °C for 5 min to obtain single LSCs for further experiments. [22] LSCs were seeded onto scaffolds at a density of 2500 cells cm 3 . Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan) was carried on 1, 3, and 7 days after inoculation.…”

Section: Methodsmentioning

confidence: 99%

“…Limbal stem cell deficiency (LSCD) represents a group of blindness causing disease characterized by conjunctivalization and vascularization of the cornea. [1,2] It is estimated that more than 12.7 million patients with moderate to severe vision loss around the world need corneal transplantation, [3] and among which 30% of those with corneal transplantation failure suffered from LSCD. More concretely, the current strategy to culture limbal epithelial transplantation (CLET), [4] of extracting few limbal stem cells DOI: 10.1002/mabi.202100441 (LSC) from the eye, and then to culture and form limbal bio-engineered tissue, has been enjoying remarkable, nonetheless, mixed success.…”

Section: Introductionmentioning

confidence: 99%

Limbal Bio‐Engineered Tissue Employing 3D Nanofiber‐Aerogel Scaffold to Facilitate LSCs Growth and Migration

Tan

Chen

Wang

et al. 2022

Macromolecular Bioscience

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Constrained by the existing scaffold inability to mimic limbal niche, limbal bio-engineered tissue constructed in vitro is challenging to be widely used in clinical practice. Here, a 3D nanofiber-aerogel scaffold is fabricated by employing thermal cross-linking electrospinned film polycaprolactone (PCL) and gelatin (GEL) as the precursor. Benefiting from the cross-linked (160 °C, vacuum) structure, the homogenized and lyophilized 3D nanofiber-aerogel scaffold with preferable mechanical strength is capable of refraining the volume collapse in humid vitro. Intriguingly, compared with traditional electrospinning scaffolds, the authors' 3D nanofiber-aerogel scaffolds possess enhanced water absorption (1100-1300%), controllable aperture (50-100 μm), and excellent biocompatibility (optical density value, 0.953 ± 0.021). The well-matched aperture and nanostructure of the scaffolds with cells enable the construction of limbal bio-engineered tissue. It is foreseen that the proposed general method can be extended to various aerogels, providing new opportunities for the development of novel limbal bio-engineered tissue.

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“… 3 There are 12.7 million people globally with moderate to severe vision loss (vision less than 20/60) resulting from corneal opacity, which can be surgically corrected, and are actively waiting for a corneal transplant. 4 However, due to the shortage of corneal graft tissue, approximately 53% people in the world have no access to corneal transplantation. 5 Deeper understanding of the corneal wound-healing process can help avoid corneal opacity and can be used to develop new treatment strategies to ease the shortage of corneal graft tissue.…”

Section: Introductionmentioning

confidence: 99%

Healing Ability of Central Corneal Epithelium in Rabbit Ocular Surface Injury Models

Zhang

Lan

Zhu

et al. 2022

Trans. Vis. Sci. Tech.

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Purpose Wound healing of the corneal epithelium mainly involves two types of cells: limbal stem/progenitor cells (LSCs) and differentiated central corneal epithelial cells (CECs). The healing ability of CECs is still debatable, and its correlated transcriptomic alterations during wound healing are yet to be elucidated. This study aimed to determine the healing ability and mechanisms underlying the actions of CECs using rabbit ocular surface injury models. Methods A central corneal ring-like residual epithelium model was used to investigate the healing ability of CECs. Uninjured and injury-stimulated LSCs and CECs were collected for transcriptomic analysis. The analysis results were verified by quantitative reverse transcriptase polymerase chain reaction, immunofluorescence staining, and two types of rabbit corneal injury models. Results During wound healing, the upregulated genes in LSCs were mostly enriched in the mitotic cell cycle–related processes, but those in CECs were mostly enriched in cell adhesion and migration. CECs could repair the epithelial defects successfully at one-time injuries. However, after repetitive injuries, the CECs repaired notably slower and failed to completely heal the defect, but the LSCs repaired even faster than the one-time injury. Conclusions Our results indicated rabbit CECs repair the epithelial defect mainly depending on migration and its proliferative ability is limited, and LSCs are the main source of regenerative epithelial cells. Translational Relevance This study provides information on gene expression in the corneal epithelium during wound healing, indicating that regulation of the cell cycle, cell adhesion, and migration may be the basis for future treatment strategies for corneal wound healing.

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In Pursuit of the Elimination of Corneal Blindness: Is Establishing Eye Banks and Training Surgeons Enough?

Cited by 28 publications

References 11 publications

Recombinant Human Nerve Growth Factor (Cenegermin)–Driven Corneal Wound Healing Process: An Evidence-Based Analysis

Recombinant Human Nerve Growth Factor (Cenegermin)–Driven Corneal Wound Healing Process: An Evidence-Based Analysis

Limbal Bio‐Engineered Tissue Employing 3D Nanofiber‐Aerogel Scaffold to Facilitate LSCs Growth and Migration

Healing Ability of Central Corneal Epithelium in Rabbit Ocular Surface Injury Models

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