In-line phase-contrast and grating-based phase-contrast synchrotron imaging study of brain micrometastasis of breast cancer

Huang, Sheng; Kou, Binquan; Chi, Yayun; Xi, Yan; Cao, Yixin; Cui, Weigang; Hu, Xin; Shao, Zhimin; Guo, Han; Fu, Yanan; Xiao, Tiqiao; Sun, Junqiang; Zhao, Jun; Wang, Yujie; Wu, Jiong

doi:10.1038/srep09418

Cited by 24 publications

(20 citation statements)

References 40 publications

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“…In particular, thanks to the high transverse coherence length of the synchrotron X-ray beam, it is possible to apply phase-contrast imaging (PCI) techniques with a simple experimental set-up. PCI has shown to be a powerful tool for the investigation of biological samples (Wilkins et al 1996;Fitzgerald, 2000;Mayo et al 2012;Mohammadi et al 2014;Huang et al 2015). Hence, synchrotron imaging may result in an improved detectability and in sharper borders around the thin membranes.…”

Section: Aims Of the Present Studymentioning

confidence: 99%

“…This makes PCI a very promising technique not only for weakly absorbing biological samples such as the membranous labyrinth, but also for (e.g.) lung tissues and (micro) metastatic tumours (Wilkins et al 1996;Fitzgerald, 2000;Mayo et al 2012;Mohammadi et al 2014;Huang et al 2015).…”

Section: Phase-contrast Imagingmentioning

confidence: 99%

“…In fact, highly collimated and partially coherent X-ray beams available at synchrotron-based hard X-ray imaging beamlines enable the use of PB-PCI in an experimental set-up without additional optical elements. Such additional elements are required for some other PCI techniques, such as grating-based PCI (Paganin et al 2002;Mohammadi et al 2014;Huang et al 2015) and analyserbased imaging (Bravin et al (2003)).…”

Section: Phase-contrast Imagingmentioning

confidence: 99%

“…; Huang et al. ). Hence, synchrotron imaging may result in an improved detectability and in sharper borders around the thin membranes.…”

Section: Introductionmentioning

confidence: 98%

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Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

Goyens

Vasilopoulou‐Kampitsi

Claes

et al. 2018

Journal of Anatomy

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The vestibular system, located in the inner ear, plays a crucial role in balance and gaze stabilisation by sensing head movements. The interconnected tubes with membranous walls of the vestibular system are located in the skull bone (the ‘membranous labyrinth’). Unfortunately, these membranes are very hard to visualise using three‐dimensional (3D) X‐ray imaging techniques. This difficulty arises due to the embedment of the membranes in the dense skull bone, the thinness of the membranes, and the small difference in X‐ray absorption between the membranes and the surrounding fluid. In this study, we compared the visualisation of very small specimens (lizard heads with vestibular systems smaller than 3 mm) by X‐ray computed micro‐tomography (μCT) based on synchrotron radiation and conventional sources. A visualisation protocol using conventional X‐ray μCT would be very useful thanks to the ease of access and lower cost. Careful optimisation of the acquisition parameters enables detection of the membranes by using μCT scanners based on conventional microfocus sources, but in some cases a low contrast‐to‐noise ratio (CNR) prevents fast and reliable segmentation of the membranes. Synchrotron radiation μCT proved to be preferable for the visualisation of the small samples with very thin membranes, because of their high demands for spatial and contrast resolution. The best contrast was obtained by using synchrotron radiation μCT working in phase‐contrast mode, leading to up to twice as high CNRs than the best conventional μCT results. The CNR of the synchrotron radiation μCT scans was sufficiently high enough to enable the construction of a 3D model by the means of semi‐automatic segmentation of the membranous labyrinth. Membrane thickness was found to range between 2.7 and 36.3 μm. Hence, the minimal membrane thickness was found to be much smaller than described previously in the literature (between 10 and 50 μm).

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Section: Aims Of the Present Studymentioning

confidence: 99%

Section: Phase-contrast Imagingmentioning

confidence: 99%

Section: Phase-contrast Imagingmentioning

confidence: 99%

“…; Huang et al. ). Hence, synchrotron imaging may result in an improved detectability and in sharper borders around the thin membranes.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

Goyens

Vasilopoulou‐Kampitsi

Claes

et al. 2018

Journal of Anatomy

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show abstract

“…X-ray phase contrast tomography (PCT) has attracted a significant interest because it utilizes phase shift signals and could provide better contrast for soft biological tissues and low atomic number samples 1–3 . The grating interferometer for differential phase contrast (DPC) imaging is among the most promising methods that can stably extract phase signals 2, 4–6 .…”

Section: Introductionmentioning

confidence: 99%

Interior tomography from differential phase contrast data via Hilbert transform based on spline functions

2016

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X-ray phase contrast imaging is an important mode due to its sensitivity to subtle features of soft biological tissues. Grating-based differential phase contrast (DPC) imaging is one of the most promising phase imaging techniques because it works with a normal x-ray tube of a large focal spot at a high flux rate. However, a main obstacle before this paradigm shift is the fabrication of large-area gratings of a small period and a high aspect ratio. Imaging large objects with a size-limited grating results in data truncation which is a new type of the interior problem. While the interior problem was solved for conventional x-ray CT through analytic extension, compressed sensing and iterative reconstruction, the difficulty for interior reconstruction from DPC data lies in that the implementation of the system matrix requires the differential operation on the detector array, which is often inaccurate and unstable in the case of noisy data. Here, we propose an iterative method based on spline functions. The differential data are first back-projected to the image space. Then, a system matrix is calculated whose components are the Hilbert transforms of the spline bases. The system matrix takes the whole image as an input and outputs the back-projected interior data. Prior information normally assumed for compressed sensing is enforced to iteratively solve this inverse problem. Our results demonstrate that the proposed algorithm can successfully reconstruct an interior region of interest (ROI) from the differential phase data through the ROI.

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Synchrotron Radiation X‐ray Imaging in Biomedical Research

Wang

Yang

2018

Synchrotron Radiation in Materials Science

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In-line phase-contrast and grating-based phase-contrast synchrotron imaging study of brain micrometastasis of breast cancer

Cited by 24 publications

References 40 publications

Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

Interior tomography from differential phase contrast data via Hilbert transform based on spline functions

Synchrotron Radiation X‐ray Imaging in Biomedical Research

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