In‐line filtration of autologous whole blood

Karger, Ralf; Schmidt, Jan; Weber, Christian Friedrich; Weiler-Lorentz, Arnulf; Frietsch, Thomas; Kretschmer, V.

doi:10.1111/j.1537-2995.2006.00801.x

Cited by 8 publications

(9 citation statements)

References 34 publications

(39 reference statements)

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“…The net benefit of this advantage, however, is unclear because leukoreduction results in a loss of RBCs in the WBC filter of up to 10 percent. 14 In conclusion, leukoreduction of AWB does not affect the infection rate and LOS in elective hip surgery. Furthermore, release of BRMs from disintegrating blood cells during storage is probably not a clinically relevant mechanism of TRIM.…”

Section: Table 3 Analyses Of Overall Infection Rate (Primary Study Ementioning

confidence: 98%

“…35,36 The findings of this trial could have immediate impact on the logistics and unfavorable cost-effectiveness of PABD programs. 37 On the other hand, quality variables of LD AWB including posttransfusion recovery are better than those of non-LD AWB, 14,38 allowing longer storage of the blood product. This might render restoration of the patients' Hb before surgery more effective.…”

Section: Table 3 Analyses Of Overall Infection Rate (Primary Study Ementioning

confidence: 99%

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Leukodepletion of autologous whole blood has no impact on perioperative infection rate and length of hospital stay

Frietsch¹,

Karger

Schöler³

et al. 2008

Transfusion

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Section: Table 3 Analyses Of Overall Infection Rate (Primary Study Ementioning

confidence: 98%

Section: Table 3 Analyses Of Overall Infection Rate (Primary Study Ementioning

confidence: 99%

Leukodepletion of autologous whole blood has no impact on perioperative infection rate and length of hospital stay

Frietsch¹,

Karger

Schöler³

et al. 2008

Transfusion

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“…Leukoreduction was one major step in the advancement of the quality of WB. Whereas leukoreduction of WB is not of clinical benefit in autologous transfusion, 23‐25 it might be beneficial in allogeneic transfusion because, in contrast to unmodified WB, 26 RBC survival of LR‐WB is comparable to that of RBCs from state‐of‐the‐art leukoreduced RBCs, 27 and labile coagulation factors are preserved during storage 13 . Thus, WB might be of value in clinical settings where disturbances of plasmatic coagulation are to be anticipated 10‐12 .…”

Section: Discussionmentioning

confidence: 99%

The influence of maintaining the correct whole blood‐to‐anticoagulant ratio during donation on the quality of leukoreduced whole blood

2011

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Section: Discussionmentioning

confidence: 99%

“…RBC units contained a high number of granulocytes. Concerns about cytokine activation during the manipulation steps cannot be excluded, although it was shown recently, that transfusion of not leucocyte depleted, autologous blood did not lead to increased transfusion reactions [23].Our primary decision to omit the reinfusion of blood ⁄ saline solution from the tubing set into the collection bag in order to avoid dilution of the recovered RBCs has to be revised, because we observed haemolysis in the recovered RBCs requiring additional plasma depletion and …”

mentioning

confidence: 99%

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

et al. 2010

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Background After large volume bone marrow (BM) harvest, donors and patients can develop severe anaemia, because collected BM can contain up to 20% of their red cell mass. In a prospective analysis, we investigated the feasibility to recover red blood cells (RBCs) from the harvested BM and investigated whether these RBC units meet the quality requirements of the European Council.Patients and Methods From 19 patients (median age 51 yrs, range 31-77) with acute myocardial infarction, who participated in the MYSTAR study, a median volume of 1299 ml (range, 700-1870 ml) BM was collected. During BM processing, mononuclear cells (MNC) were separated using the Cobe SpectraÔ apheresis system and the residual RBCs were collected in a separate bag. The quality of the collected RBCs was assessed by measuring LDH, free haemoglobin, potassium and lactate. Haemolysis was calculated and the intracellular concentration of ATP, ADP, AMP was determined by HPLC.Results RBC units recovered from BM after MNC separation had a mean volume of 312 ± 95 ml with a haematocrit of 47 ± 8AE9%, a haemoglobin content of 51 ± 15 g per unit, a haemolysis of 0AE15 ± 0AE005%, a pH of 6AE8 ± 0AE007 and an intracellular ATP concentration of 135 pmol ⁄ 10 6 RBC ± 41, which is comparable with freshly collected packed red blood cells (PRBCs).Conclusion RBCs, collected from bone marrow harvests, can be used for autologous blood support to minimize allogeneic blood transfusions in donors and patients after large volume BM donation.Key words: blood sparing measurements, bone marrow harvest, bone marrow processing, salvage of red blood cells. IntroductionLarge volume bone marrow (BM) harvest is an alternative to G-CSF-induced peripheral blood stem cell collection in patients or donors who are poor mobilizers, in the paediatric setting or in the regenerative medicine, when patients with myocardial infarction are intended to receive stem cells intracardially [1][2][3][4]. Donors and patients are at risk to develop a severe anaemia because collected BM can contain up to 20% of their red cell mass. To reduce the risk of anaemia after BM donation, at least one unit of autologous blood should be collected prior to the harvest procedure [5]. Alternatively, certain clinicians place the donors on supplemental iron plus rhEPO, transfuse their donors with allogeneic RBC units or tolerate anaemia when not severe [6]. Previous studies have shown that a recovery of approximately 90% of the harvested BM-RBCs decreased the homologous transfusion requirement of healthy BM donors significantly [7]. When paediatric BM donors were investigated and BM-RBCs were separated by density gradient centrifugation using Ficoll-Hypaque, similar results were In a prospective analysis, we assessed the extent of cell lesions occurring during manipulation by measuring the release of lactate dehydrogenase (LDH), free haemoglobin (fHb), potassium (K+) and lactate at each manipulation step. Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate mar...

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In‐line filtration of autologous whole blood

Cited by 8 publications

References 34 publications

Leukodepletion of autologous whole blood has no impact on perioperative infection rate and length of hospital stay

Leukodepletion of autologous whole blood has no impact on perioperative infection rate and length of hospital stay

The influence of maintaining the correct whole blood‐to‐anticoagulant ratio during donation on the quality of leukoreduced whole blood

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

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