In-house Validation of a Novel Capillary Electrophoretic Method: Uncertainty Factors

Monitoring wood-related biorefinery process streams is important as the composition of raw material and its variations have a great impact on the production outcome as well as on the process control. In this paper, the applicability of a simple type of capillary zone electrophoresis method to profiling heterogeneous woodbased samples was studied by grouping the peaks in the overall electropherograms. A comparison between 210 and 270 nm as an example confirmed the identification of a monosaccharide peak in the carbohydrate electropherogram. The analytical method applied in this study was found suitable, firstly, in profiling the samples based on correlation of multiple detection wavelengths and, secondly, in characterizing the total carbohydrate composition by grouping detected peaks by the compound type. Compounds were detected in their extracted form without timeconsuming pretreatment procedures. Differences in the raw material and process conditions were successfully identified by comparing characteristic peaks in the sample electropherograms of an example case.

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Profiling of water-soluble carbohydrates in pine and spruce extracts by capillary zone electrophoresis with direct UV detection

Kaijanen

Metsämuuronen

Рейникайнен

et al. 2015

Wood Sci Technol

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Ultraviolet Detection of Monosaccharides: Multiple Wavelength Strategy to Evaluate Results after Capillary Zone Electrophoretic Separation

Kaijanen

Paakkunainen

Pietarinen

et al. 2015

International Journal of Electrochemical Science

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Detection of Novel Carbohydrate-Related Compounds in Aqueous Samples Using a Capillary Electrophoretic Profiling Method

Kaijanen

Рейникайнен

Pietarinen

et al. 2014

Separation Science and Technology

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In-house Validation of a Novel Capillary Electrophoretic Method: Uncertainty Factors

Cited by 4 publications

References 16 publications

Profiling of water-soluble carbohydrates in pine and spruce extracts by capillary zone electrophoresis with direct UV detection

Profiling of water-soluble carbohydrates in pine and spruce extracts by capillary zone electrophoresis with direct UV detection

Ultraviolet Detection of Monosaccharides: Multiple Wavelength Strategy to Evaluate Results after Capillary Zone Electrophoretic Separation

Detection of Novel Carbohydrate-Related Compounds in Aqueous Samples Using a Capillary Electrophoretic Profiling Method

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