In-Depth Qualitative Analysis of Lime Essential Oils Using the Off-Line Combination of Normal Phase High Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Quadrupole Mass Spectrometry

Zoccali, Mariosimone; Giocastro, Barbara; Bonaccorsi, Ivana; Trozzi, Alessandra; Tranchida, Peter Q.; Mondello, Luigi

doi:10.3390/foods8110580

Cited by 8 publications

(5 citation statements)

References 14 publications

(25 reference statements)

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“…Another powerful approach that also holds the possibility for automation and on‐line configuration, is the use of liquid chromatography as a pre‐fractionation step before the GC×GC separation. A detailed qualitative analysis of the volatile fractions of mandarin and lime essential oils was achieved by combining HPLC and GC×GC–MS [112,113], although in an off‐line configuration in these examples. The pre‐separation using a normal phase HPLC process aimed at the isolation of the oxygenated compounds contributing to the composition of the essential oils.…”

Section: Non‐targeted Analysis Applicationsmentioning

confidence: 99%

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Franchina

Zanella

Dubois

et al. 2020

J of Separation Science

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In this review, we consider and discuss the affinity and complementarity between a generic sample preparation technique and the comprehensive two‐dimensional gas chromatography process. From the initial technical development focus (e.g., on the GC×GC and solid‐phase microextraction techniques), the trend is inevitably shifting toward more applied challenges, and therefore, the preparation of the sample should be carefully considered in any GC×GC separation for an overreaching research. We highlight recent biomedical, food, and plant applications (2016–July 2020), and specifically those in which the combination of tailored sample preparation methods and GC×GC–MS has proven to be beneficial in the challenging aspects of non‐targeted analysis. Specifically on the sample preparation, we report on gas‐phase, solid‐phase, and liquid‐phase extractions, and derivatization procedures that have been used to extract and prepare volatile and semi‐volatile metabolites for the successive GC×GC analysis. Moreover, we also present a milestone section reporting the early works that pioneered the combination of sample preparation techniques with GC×GC for non‐targeted analysis.

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Section: Non‐targeted Analysis Applicationsmentioning

confidence: 99%

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Franchina

Zanella

Dubois

et al. 2020

J of Separation Science

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“…It produced a thick dark green extract with a pleasant odour and a yield of 6.21%. The main component, according to high-performance liquid chromatography, 16 (Agilent 1260 Infinity, Agilent Technologies Inc., USA) was rosmarinic acid.…”

Section: Methodsmentioning

confidence: 99%

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Badekova

Атажанова

Kačergius

et al. 2021

Journal of Taibah University Medical Sciences

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Objectives This study aims to formulate a new Origanum vulgare anti-caries dental gel with high antimicrobial activity. Methods O. vulgare essential oil was extracted using hydro-distillation, ethanol extraction, gas chromatography/mass spectrometry, and high-performance liquid chromatography methods. Antimicrobial activity of the produced gels with oregano oil and extract was determined through the disco-diffusion method. The effectiveness of O. vulgare essential oil was tested in vitro for Streptococcus mutans biofilm using colorimetric analysis. Results O. vulgare essential oil inhibited the growth of S. mutans biofilm by 98% compared with unexposed control bacteria ( p <0.05 ). Five samples of anti-caries gel (ACDG1, ACDG2, ACDG3, ACDG4, ACDG5) were formulated using the obtained oregano essential oil and extract. Based on the microbiological study results, the ACDG1 and ACDG3 gel samples exhibited high antimicrobial activity against the gram-positive bacterial strains of Staphylococcus aureus and Bacillus subtilis and the yeast fungus Candida albicans and moderate antimicrobial activity against the gram-negative strains of Escherichia coli and Pseudomonas aeruginosa . Conclusion Based on the results of this study, the ACDG3 sample may be considered an anti-caries gel owing to its high antimicrobial activity. This sample has good organoleptic properties compared to other samples, produces relatively high antimicrobial activity, and guards against cariogenic biofilms of S. mutans.

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“…When a scan is taken on the slope of the peak, the concentration of the analyte will vary, resulting in distorted and varying spectra. Rapid-scanning Q-MS systems have become available, but they are only occasionally used in contemporary GC × GC-MS practice [111][112][113][114][115]. Triple-quadrupole (QqQ) instruments have been demonstrated for online use with GC × GC [116][117][118].…”

Section: Developments In Gc × Gc Detectionmentioning

confidence: 99%

Comprehensive two‐dimensional gas chromatography— A discussion on recent innovations

Milani,

van Gilst,

Pirok

et al. 2023

J of Separation Science

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Although comprehensive 2‐D GC is an established and often applied analytical method, the field is still highly dynamic thanks to a remarkable number of innovations. In this review, we discuss a number of recent developments in comprehensive 2‐D GC technology. A variety of modulation methods are still being actively investigated and many exciting improvements are discussed in this review. We also review interesting developments in detection methods, retention modeling, and data analysis.

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In-Depth Qualitative Analysis of Lime Essential Oils Using the Off-Line Combination of Normal Phase High Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Quadrupole Mass Spectrometry

Cited by 8 publications

References 14 publications

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Comprehensive two‐dimensional gas chromatography— A discussion on recent innovations

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