In-depth investigation of the interaction between DNA and nano-sized graphene oxide

Lee, Jieon; Yim, Yeajee; Kim, Seongchan; Choi, Mun Kee; Choi, Byong‐Seok; Lee, Younghoon; Min, Dal‐Hee

doi:10.1016/j.carbon.2015.07.093

Cited by 57 publications

(44 citation statements)

References 44 publications

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“…These properties make GO capable of (1) strong binding with biomolecules through pipi stacking and/or hydrogen bonding interactions and (2) the fluorescence quenching of nearby fluorescent dye by the process of energy transfer from the excited state of the dye to GO [29][30][31][32][33][34]. Particularly, preferential binding of single stranded nucleic acid (NA) on GO compared to double-stranded NA was one of the most popular principles in the fluorescent GO utilized biosensors [35][36][37][38]. Second, GO is considered as a potential matrix for LDI-MS that can be employed as an alternative to conventional small molecule matrix in bioanalysis.…”

Section: General Strategies For Go-based Biosensorsmentioning

confidence: 99%

Biosensors based on graphene oxide and its biomedical application

Lee

Kim

et al. 2016

Advanced Drug Delivery Reviews

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Graphene oxide (GO) is one of the most attributed materials for opening new possibilities in the development of next generation biosensors. Due to the coexistence of hydrophobic domain from pristine graphite structure and hydrophilic oxygen containing functional groups, GO exhibits good water dispersibility, biocompatibility, and high affinity for specific biomolecules as well as properties of graphene itself partly depending on preparation methods. These properties of GO provided a lot of opportunities for the development of novel biological sensing platforms, including biosensors based on fluorescence resonance energy transfer (FRET), laser desorption/ionization mass spectrometry (LDI-MS), surface-enhanced Raman spectroscopy (SERS), and electrochemical detection. In this review, we classify GO-based biological sensors developed so far by their signal generation strategy and provide the comprehensive overview of them. In addition, we offer insights into how the GO attributed in each sensor system and how they improved the sensing performance.

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Section: General Strategies For Go-based Biosensorsmentioning

confidence: 99%

Biosensors based on graphene oxide and its biomedical application

Lee

Kim

et al. 2016

Advanced Drug Delivery Reviews

Self Cite

326

173

View full text Add to dashboard Cite

show abstract

“…7 To disperse in water and interface with biopolymers, graphene oxide (GO) is often used. [8][9][10] The interaction between GO and DNA has been extensively studied, [11][12][13][14][15] which has inspired the exploration of various other 2D materials.…”

Section: Introductionmentioning

confidence: 99%

Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing

et al. 2017

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Interfacing DNA with two-dimensional (2D) materials has been intensely researched for various analytical and biomedical applications. Most of such studies were performed on graphene oxide (GO), and two metal dichalcogenides, MoS2 and WS2; all of them can all adsorb single-stranded DNA. However, they like use different surface forces for adsorption based on their chemical structures. In this work, fluorescently labeled DNA oligonucleotides were used and their adsorption capacity and kinetics were studied as a function of ionic strength, DNA length and sequence. Desorption of DNA from these surfaces were also measured. DNA is more easily desorbed from GO by various denaturing agents, while surfactants yield more desorption from MoS2 and WS2. Our results are consistent with that DNA can be adsorbed by GO via π-π stacking and hydrogen bonding, MoS2 and WS2 mainly use van der Waals force for adsorption. Finally, fluorescent DNA probes were adsorbed by these 2D materials for detecting the complementary DNA. For this assay, GO gave the highest sensitivity, while they all showed a similar detection limit. This study has enhanced our fundamental understanding of DNA adsorption by two important types of 2D materials and is useful for further rational optimization of their analytical and biomedical applications.3

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“…21 Fundamental studies on the interaction between DNA and GO were also carried out. 11,[22][23][24][25][26][27][28][29][30][31][32] GO is a loosely defined material, and the oxygen content can vary quite a lot depending on the preparation condition. The adsorption affinity of DNA is likely to depend on the oxygen content.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

et al. 2016

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Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the condition of preparation. Sometimes, a further reduction step is intentionally performed to decrease the oxygen content and the resulting material is called reduced GO (rGO). In this work, DNA adsorption and desorption from GO and rGO is systematically compared. Under the same salt concentration, DNA adsorbs slightly faster with a 2.6-fold higher capacity on rGO. At the same time, adsorbed DNA on rGO is more resistant to desorption induced by temperature, pH, urea, and organic solvents. Various lengths and sequences of DNA probes have been tested. When its complementary DNA (cDNA) is added as a model target analyte, the rGO sample has a higher signal-to-background and signalto-noise ratio, while the GO sample has a slightly higher absolute signal increase and faster signaling kinetics. Adsorbed DNAs on GO or rGO are still susceptible to non-specific displacement by other DNA and proteins. Overall, while rGO adsorb DNA more tightly, it allows efficient DNA sensing with an extremely low background signal.

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In-depth investigation of the interaction between DNA and nano-sized graphene oxide

Cited by 57 publications

References 44 publications

Biosensors based on graphene oxide and its biomedical application

Biosensors based on graphene oxide and its biomedical application

Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

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In-depth investigation of the interaction between DNA and nano-sized graphene oxide

Cited by 57 publications

References 44 publications

Biosensors based on graphene oxide and its biomedical application

Biosensors based on graphene oxide and its biomedical application

Comparison of MoS2, WS2, and Graphene Oxide for DNA Adsorption and Sensing

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

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Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing