In Cellulo Evaluation of the Therapeutic Potential of NHC Platinum Compounds in Metastatic Cutaneous Melanoma

Charignon, Elsa; Bouché, Mathilde; Clave-Darcissac, Caroline; Dahm, Georges; Ichim, Gabriel; Clotagatide, Anthony; Mertani, Hichem C.; Télouk, Philippe; Caramel, Julie; Diaz, Jean‐Jacques; Bellemin‐Laponnaz, Stéphane; Bouvet, Philippe; Billotey, Claire

doi:10.3390/ijms21217826

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…In addition, the study of the BRAF-inhibitor-resistant variant of A375 cells demonstrated a significant reduction in dacarbazine effectiveness. The value of EC 50 for 72 h of treatment was found to be 288.8 µM [ 60 ]. The above data from in vitro studies show some of the limitations and relatively low efficacy of dacarbazine, also observed in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

The Anticancer Potential of Doxycycline and Minocycline—A Comparative Study on Amelanotic Melanoma Cell Lines

Rok

Rzepka

Kowalska

et al. 2022

IJMS

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Malignant melanoma is still a serious medical problem. Relatively high mortality, a still-growing number of newly diagnosed cases, and insufficiently effective methods of therapy necessitate melanoma research. Tetracyclines are compounds with pleiotropic pharmacological properties. Previously published studies on melanotic melanoma cells ascertained that minocycline and doxycycline exerted an anti-melanoma effect. The purpose of the study was to assess the anti-melanoma potential and mechanisms of action of minocycline and doxycycline using A375 and C32 human amelanotic melanoma cell lines. The obtained results indicate that the tested drugs inhibited proliferation, decreased cell viability, and induced apoptosis in amelanotic melanoma cells. The treatment caused changes in the cell cycle profile and decreased the intracellular level of reduced thiols and mitochondrial membrane potential. The exposure of A375 and C32 cells to minocycline and doxycycline triggered the release of cytochrome c and activated initiator and effector caspases. The anti-melanoma effect of analyzed drugs appeared to be related to the up-regulation of ERK1/2 and MITF. Moreover, it was noticed that minocycline and doxycycline increased the level of LC3A/B, an autophagy marker, in A375 cells. In summary, the study showed the pleiotropic anti-cancer action of minocycline and doxycycline against amelanotic melanoma cells. Considering all results, it could be concluded that doxycycline was a more potent drug than minocycline.

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Section: Discussionmentioning

confidence: 99%

The Anticancer Potential of Doxycycline and Minocycline—A Comparative Study on Amelanotic Melanoma Cell Lines

Rok

Rzepka

Kowalska

et al. 2022

IJMS

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“…No studies have been conducted on the bystander effect as a result of ultrasound exposure combined with chemotherapy agents [ 20 , 21 ]. Therefore, the aim and the novelty of the present study are to evaluate the contribution of the US in the presence of cisplatin to induce the bystander effect, and also this study was conducted on melanoma as the deadliest type of skin cancer with resistance to conventional therapies such as chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Bystander Effect of Therapeutic Ultrasound in the Presence of Cisplatin: An in Vitro Study on Human Melanoma Cells

2023

J Biomed Phys Eng

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Background: In the bystander effect, non-irradiated cells receive biological signals from adjacent irradiated cells and undergo a variety of alterations, considered recently in non-ionizing irradiation like ultrasound waves. In this study, the bystander effect of therapeutic ultrasound exposure alone and in combination with cisplatin was determined. Objective: This study aims to determine the bystander effect caused by ultrasound and cisplatin. Material and Methods: This experimental study was conducted on the human melanoma cell line including two groups of target and bystander cells. The target cell group was divided into three sub-groups of ultrasound irradiation alone, cisplatin alone, and ultrasound irradiation in the presence of cisplatin that the culture medium of these three groups of cells was transferred to the bystander cell group using the medium transfer technique. Then, apoptotic bystander cells and the expression of P53 and HO-1 in target and bystander groups were measured. Results: The results of the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis assay showed that cell death in target and bystander groups receiving the ultrasound with cisplatin is higher than in the ultrasound without cisplatin. PCR (the polymerase chain reaction) results in the target and bystander groups receiving treatments with increased expression of the P53 gene. Target and bystander groups receiving the ultrasound without cisplatin showed a decrease in HO-1 gene expression, while the ultrasound with cisplatin showed an increase in the HO-1 gene compared to the control group. Conclusion: Combining ultrasound with ultrasound and without it can transfer bystander signals to the cells that are not directly treated.

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Synthesis, structure and anticancer activity of three platinum(II) complexes with 2-phenylpyridine derivatives

Niu

Ren

Hou

et al. 2021

Inorganic Chemistry Communications

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In Cellulo Evaluation of the Therapeutic Potential of NHC Platinum Compounds in Metastatic Cutaneous Melanoma

Cited by 3 publications

References 35 publications

The Anticancer Potential of Doxycycline and Minocycline—A Comparative Study on Amelanotic Melanoma Cell Lines

The Anticancer Potential of Doxycycline and Minocycline—A Comparative Study on Amelanotic Melanoma Cell Lines

Bystander Effect of Therapeutic Ultrasound in the Presence of Cisplatin: An in Vitro Study on Human Melanoma Cells

Synthesis, structure and anticancer activity of three platinum(II) complexes with 2-phenylpyridine derivatives

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