Impulsive stimulated Brillouin microscopy for non-contact, fast mechanical investigations of hydrogels

Krug, Benedikt; Koukourakis, Nektarios; Czarske, J.

doi:10.1364/oe.27.026910

Cited by 38 publications

(29 citation statements)

References 54 publications

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“…When the frequency detuning of these lasers is around the Brillouin shift of the sample light is scattered to/ from the probe beam, resulting in stimulated Brillouin gain/loss. Here, it is worth noting that the active stimulation of phonons in ST can result in measured attenuation coefficients and thus linewidths which are different from those measured by spontaneous Brillouin scattering (Remer and Bilenca 2016;Krug et al 2019). Nevertheless, stimulated techniques offer unprecedented and exciting advantages, such as a superior spectral resolution and access to the mass density-two properties that can in principle enable one to more accurately distinguish different biomechanical constituents within the measurement volume as well as to quantify the obtained spectra in terms of mechanical modulus.…”

Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 87%

“…Current physical approaches for implementing BM can broadly be divided into two categories, depending on whether the photons scatter off spontaneous or coherently driven phonons. The latter, which are also referred to as "stimulated" techniques (ST), can in turn be divided into two categories: stimulated Brillouin scattering (SBS) (Ballmann et al 2015;Remer and Bilenca 2016;Remer et al 2019), which currently employs tunable continuous-wave (cw) lasers, and impulsive stimulated Brillouin scattering (ISBS) (Ballmann et al 2017;Krug et al 2019), which is based on ultrashort pulse laser excitation. While most realizations to date utilize spontaneous scattering (e.g., (Scarcelli and Yun 2007;Scarponi et al 2017)), recent technical developments in the field of SBS have sparked considerable interest and prompted our community to critically evaluate and discuss which of these approaches is the most promising one in terms of desirable acquisition speeds as well as spatial and spectral resolution, while maintaining sample viability.…”

Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 99%

“…Hence, we believe these developments may provide future research avenues in biology and help establish ST as a complementary method to current approaches based on spontaneous Brillouin scattering. Current ST implementations (Remer et al 2019;Krug et al 2019) however still require high laser powers and a "transmission" geometry, i.e., simultaneous optical access to the probed region in a sample from opposite sides, which can sometimes prove undesirable. Though the high laser powers are somewhat mitigated by the use of Near Infra-red (NIR) laser sources (Remer et al 2017), in current implementations these conditions can prove restrictive for studying non-transparent or photosensitive samples, or for studies over extended time periods.…”

Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 99%

“…In this direction, it is our current standpoint that better experimental design and more stringent controls are needed, as cellular mechanics are susceptible to changes on the sub-second time scale and Table 1 Current performance parameters for major variants of Brillouin microscopy implementations. All parameters were obtained on water samples, except (Krug et al 2019), and from references or supplied and updated by the respective authors. ISBS, impulsive stimulated Brillouin scattering; VIPA, virtually imaged phase array; TFPI, tandem multi-pass Fabry-Pérot interferometer.…”

Section: Strategies For Properly Assessing and Avoiding Photodamagementioning

confidence: 99%

“…ISBS, impulsive stimulated Brillouin scattering; VIPA, virtually imaged phase array; TFPI, tandem multi-pass Fabry-Pérot interferometer. Dwell time for ISBS represents effective measurement time for (Krug et al 2019). Relative precision is defined as the ratio of the instrument's precision to typical Brillouin shifts measured (e.g., water).…”

Section: Strategies For Properly Assessing and Avoiding Photodamagementioning

confidence: 99%

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Recent progress and current opinions in Brillouin microscopy for life science applications

et al. 2020

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Many important biological functions and processes are reflected in cell and tissue mechanical properties such as elasticity and viscosity. However, current techniques used for measuring these properties have major limitations, such as that they can often not measure inside intact cells and/or require physical contact-which cells can react to and change. Brillouin light scattering offers the ability to measure mechanical properties in a non-contact and label-free manner inside of objects with high spatial resolution using light, and hence has emerged as an attractive method during the past decade. This new approach, coined "Brillouin microscopy," which integrates highly interdisciplinary concepts from physics, engineering, and mechanobiology, has led to a vibrant new community that has organized itself via a European funded (COST Action) network. Here we share our current assessment and opinion of the field, as emerged from a recent dedicated workshop. In particular, we discuss the prospects towards improved and more bio-compatible instrumentation, novel strategies to infer more accurate and quantitative mechanical measurements, as well as our current view on the biomechanical interpretation of the Brillouin spectra.

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Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 87%

Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 99%

Section: Approaches and Future Prospects For High-performance Instrummentioning

confidence: 99%

Section: Strategies For Properly Assessing and Avoiding Photodamagementioning

confidence: 99%

Section: Strategies For Properly Assessing and Avoiding Photodamagementioning

confidence: 99%

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Recent progress and current opinions in Brillouin microscopy for life science applications

et al. 2020

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Translating Therapeutic Microgels into Clinical Applications

Kittel

Kuehne

Laporte

2021

Adv Healthcare Materials

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Microgels are crosslinked, water‐swollen networks with a 10 nm to 100 µm diameter and can be modified chemically or biologically to render them biocompatible for advanced clinical applications. Depending on their intended use, microgels require different mechanical and structural properties, which can be engineered on demand by altering the biochemical composition, crosslink density of the polymer network, and the fabrication method. Here, the fundamental aspects of microgel research and development, as well as their specific applications for theranostics and therapy in the clinic, are discussed. A detailed overview of microgel fabrication techniques with regards to their intended clinical application is presented, while focusing on how microgels can be employed as local drug delivery materials, scavengers, and contrast agents. Moreover, microgels can act as scaffolds for tissue engineering and regeneration application. Finally, an overview of microgels is given, which already made it into pre‐clinical and clinical trials, while future challenges and chances are discussed. This review presents an instructive guideline for chemists, material scientists, and researchers in the biomedical field to introduce them to the fundamental physicochemical properties of microgels and guide them from fabrication methods via characterization techniques and functionalization of microgels toward specific applications in the clinic.

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Multivariate analysis of Brillouin imaging data by supervised and unsupervised learning

Xiang

Seow

Paterson

et al. 2021

Journal of Biophotonics

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Brillouin imaging relies on the reliable extraction of subtle spectral information from hyperspectral datasets. To date, the mainstream practice has been to use line fitting of spectral features to retrieve the average peak shift and linewidth parameters. Good results, however, depend heavily on sufficient signal-to-noise ratio and may not be applicable in complex samples that consist of spectral mixtures. In this work, we thus propose the use of various multivariate algorithms that can be used to perform supervised or unsupervised analysis of the hyperspectral data, with which we explore advanced image analysis applications, namely unmixing, classification and segmentation in a phantom and live cells.The resulting images are shown to provide more contrast and detail, and obtained on a timescale $10 2 faster than fitting. The estimated spectral parameters are consistent with those calculated from pure fitting.

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Impulsive stimulated Brillouin microscopy for non-contact, fast mechanical investigations of hydrogels

Cited by 38 publications

References 54 publications

Recent progress and current opinions in Brillouin microscopy for life science applications

Recent progress and current opinions in Brillouin microscopy for life science applications

Translating Therapeutic Microgels into Clinical Applications

Multivariate analysis of Brillouin imaging data by supervised and unsupervised learning

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