Speckle noise is a ubiquitous artifact that limits the interpretation of optical coherence tomography images. Here we apply various speckle-reduction digital filters to optical coherence tomography images and compare their performance. Our results indicate that shift-invariant, nonorthogonal wavelet-transform-based filters together with enhanced Lee and adaptive Wiener filters can significantly reduce speckle and increase the signal-to-noise ratio, while preserving strong edges. The speckle reduction capabilities of these filters are also compared with speckle reduction from incoherent angular compounding. Our results suggest that by using these digital filters, the number of individual angles required to attain a certain level of speckle reduction can be decreased.
Long wavelength lasers and semiconductor optical amplifiers based on InAs quantum wire-/dot-like active regions were developed on InP substrates dedicated to cover the extended telecommunication wavelength range between 1.4 and 1.65 µm. In a brief overview different technological approaches will be discussed, while in the main part the current status and recent results of quantum-dash lasers are reported. This includes topics like dash formation and material growth, device performance of lasers and optical amplifiers, static and dynamic properties and fundamental material and device modelling.
Noncontact label-free biomechanical imaging is a crucial tool for unraveling the mechanical properties of biological systems, which play critical roles in the fields of engineering, physics, biology and medicine; yet, it represents a significant challenge in microscopy. Spontaneous Brillouin microscopy meets this challenge, but often requires long acquisition times or lacks high specificity for detecting biomechanical constituents with highly overlapping Brillouin bands. We developed stimulated Brillouin scattering (SBS) microscopy that provides intrinsic noncontact biomechanical contrast and generates mechanical cross-sectional images inside large specimens, with high mechanical specificity and pixel dwell times that are >10-fold improved over those of spontaneous Brillouin microscopy. We used SBS microscopy in different biological applications, including the quantification of the high-frequency complex longitudinal modulus of the pharyngeal region of live wild-type Caenorhabditis elegans nematodes, imaging of the variations in the highfrequency viscoelastic response to osmotic stress in the head of living worms, and in vivo mechanical contrast mesoscopy of developing nematodes. Main TextLabel-free biomechanical imaging has long used a variety of techniques, including atomic-force microscopy, multiphoton microscopy, and optical coherence elastography [1][2][3][4][5][6] , that obtain mechanical images with high spatial resolution, but require contact or external mechanical stimulation of the sample. Spontaneous Brillouin microscopy 7-20 circumvents these requirements by measuring the so-called Brillouin shifts B and linewidths , which are the frequency shifts and linewidths of light backscattered inelastically from gigahertz-frequency longitudinal acoustic phonons characteristic to the different viscoelastic constituents of the material. However, spontaneous Brillouin microscopy often demands long acquisition times due to the low efficiency of spontaneous Brillouin scattering in biological matter, or suffers from limited mechanical specificity because of the relatively low spectrometer resolution of spontaneous Brillouin microscopes, making it difficult to specifically detect biomechanical constituents with highly overlapping Brillouin bands.
Necrotic-core fibroatheromas (NCFA) with thin, mechanically weak fibrous caps overlying lipid cores comprise the majority of plaques that rupture and cause acute myocardial infarction. Laser speckle imaging (LSI) has been recently demonstrated to enable atherosclerotic plaque characterization with high accuracy. We investigate spatio-temporal analysis of LSI data, in conjunction with diffusion theory and Monte Carlo modeling of light transport, to estimate fibrous cap thickness in NCFAs. Time-varying laser speckle images of 20 NCFAs are selected for analysis. Spatio-temporal intensity fluctuations are analyzed by exponential fitting of the windowed normalized cross-correlation of sequential laser speckle patterns to obtain the speckle decorrelation time constant, tau(rho), as a function of distance rho from the source entry location. The distance, rho', at which tau(rho) dropped to 65% of its maximum value is recorded. Diffusion theory and Monte Carlo models are utilized to estimate the maximum photon penetration depth, zmax(rho'), for a distance equal to rho', measured from LSI. Measurements of zmax(rho') correlate well with histological measurements of fibrous cap thickness (R=0.78, p<0.0001), and paired t-tests show no significant difference between the groups (p=0.4). These results demonstrate that spatio-temporal LSI may allow the estimation of fibrous cap thickness in NCFAs, which is an important predictor of plaque stability.
Many important biological functions and processes are reflected in cell and tissue mechanical properties such as elasticity and viscosity. However, current techniques used for measuring these properties have major limitations, such as that they can often not measure inside intact cells and/or require physical contact-which cells can react to and change. Brillouin light scattering offers the ability to measure mechanical properties in a non-contact and label-free manner inside of objects with high spatial resolution using light, and hence has emerged as an attractive method during the past decade. This new approach, coined "Brillouin microscopy," which integrates highly interdisciplinary concepts from physics, engineering, and mechanobiology, has led to a vibrant new community that has organized itself via a European funded (COST Action) network. Here we share our current assessment and opinion of the field, as emerged from a recent dedicated workshop. In particular, we discuss the prospects towards improved and more bio-compatible instrumentation, novel strategies to infer more accurate and quantitative mechanical measurements, as well as our current view on the biomechanical interpretation of the Brillouin spectra.
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