Improving the viability of tissue‐resident stem cells using an organ‐preservation solution

Suzuki, Takaya; Ota, Chiharu; Fujino, Naoya; Tando, Yukiko; Suzuki, Satoshi; Yamada, Mitsuhiro; Kondo, Takashi; Okada, Yoshinori; Kubo, Hiroshi

doi:10.1002/2211-5463.12748

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…Its chloride-poor derivative solution 2 yielded superior protection for rat hepatocytes during prolonged cold storage (24). In addition, use of chloride-rich preservation solutions was found to be beneficial for the preservation of human epithelial cell lines and mice lung tissue samples after cold storage for 3 days (30).…”

Section: Discussionmentioning

confidence: 99%

Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution

Tigges¹,

Eggerbauer²,

Worek³

et al. 2021

American Journal of Physiology-Lung Cellular and Molecular Phys

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Precision cut lung slices (PCLS) are used as ex vivo model of the lung to fill the gap between in vitro and in vivo experiments. To allow optimal utilization of PCLS, possibilities to prolong slice viability via cold storage using optimized storage solutions were evaluated. Rat PCLS were cold stored in DMEM/F-12 or two different preservation solutions for up to 28 days at 4 °C. After rewarming in DMEM/F-12, metabolic activity, live/dead staining and mitochondrial membrane potential was assessed to analyze overall tissue viability. Single cell suspensions were prepared and proportions of CD45+, EpCAM+, CD31+ and CD90+ cells analyzed. As functional parameters, TNF-α expression was analyzed to detect inflammatory activity and bronchoconstriction after acetylcholine stimulus. After 14 days cold storage, viability and mitochondrial membrane potential were significantly better preserved after storage in solution 1 (potassium chloride rich) and solution 2 (potassium- and lactobionate-rich analog) compared to DMEM/F-12. Analysis of cell populations revealed efficient preservation of EpCAM+, CD31+ and CD90+ cells. Proportion of CD45+ cells decreased during cold storage but was better preserved by both modified solutions than by DMEM/F-12. PCLS stored in solution 1 responded substantially longer to inflammatory stimulation than those stored in DMEM/F-12 or solution 2. Analysis of bronchoconstriction revealed total loss of function after 14 days storage in DMEM/F-12 but in contrast, a good response in PCLS stored in the optimized solutions. An improved base solution with a high potassium chloride concentration optimizes cold storage of PCLS and allows shipment between laboratories and stockpiling of tissue samples.

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Section: Discussionmentioning

confidence: 99%

Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution

Tigges¹,

Eggerbauer²,

Worek³

et al. 2021

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…In the case of tissue transport for cell therapies, culture mediums such as DMEM and PBS are widely used. ECS, UW solution, DMEM and PBS all require cool preservation at 4 °C [2]. It should be noted that cold-chain maintenance remains a major hurdle in developing countries and during overseas transport [16].…”

Section: Discussionmentioning

confidence: 99%

“…This, in turn, necessitates effective methodologies for preserving the viability and characteristics of the resident tissue chondrocytes for culture expansion [1]. Furthermore, transportation techniques must facilitate the transportation of clinical tissue samples from distant locations, even from overseas, while maintaining cell viability and native characteristics when samples are processed after transport for cell therapy applications [2]. Phosphate-buffered saline (PBS), Dulbecco's Modified Eagle's Medium (DMEM) and Euro-Collins solution (ECS) represent common benchmark tissue preservation/transportation solutions [2].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, transportation techniques must facilitate the transportation of clinical tissue samples from distant locations, even from overseas, while maintaining cell viability and native characteristics when samples are processed after transport for cell therapy applications [2]. Phosphate-buffered saline (PBS), Dulbecco's Modified Eagle's Medium (DMEM) and Euro-Collins solution (ECS) represent common benchmark tissue preservation/transportation solutions [2]. All three solutions require temperature maintenance at 4 °C during transportation and preservation.…”

Section: Introductionmentioning

confidence: 99%

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An efficient polymer cocktail-based transportation method for cartilage tissue, yielding chondrocytes with enhanced hyaline cartilage expression during in vitro culturing

Katoh

Yoshioka²,

Suzuki

et al. 2021

Preprint

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Chondrocytes are used in cell-based therapies such as autologous chondrocyte implantation (ACI) and matrix-associated cartilage implantation (MACI). To transport the cartilage tissue to the laboratory for in vitro culturing, phosphate-buffered saline (PBS), Euro-Collins solution (ECS) and Dulbecco Modified Eagle Medium (DMEM) are commonly employed at 4-8 deg C. In this study, eight samples of human cartilage biopsy tissues from elderly patients with severe osteoarthritis undergoing arthroscopy, which would otherwise have been discarded, were used. The cartilage tissue samples were compared to assess the cell yield between two transportation groups: i) a thermo-reversible gelation polymer (TGP) based method without cool preservation (~25 deg C) and ii) ECS transport at 4 deg C. These samples were subjected to in vitro culture in a two-dimensional (2D) monolayer for two weeks and subsequently in a three-dimensional (3D) TGP scaffold for six weeks. The cell count obtained from the tissues transported in TGP was higher (0.2 million cells) than those transported in ECS (0.08 million cells) both after initial processing and after in vitro culturing for 2 weeks in 2D (18 million cells compared with 10 million cells). In addition, mRNA quantification demonstrated significantly higher expression of Col2a1 and SOX-9 in 3D-TGP cultured cells and lower expression of COL1a1 in RT-PCR, characteristic of the hyaline cartilage phenotype, than in 2D culture. This study confirms that the TGP cocktail is suitable for both the transport of human cartilage tissue and for in vitro culturing to yield better-quality cells for use in regenerative therapies.

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“…On the other hand, extracellular media is directly influencing living cells' viability. Especially in frail skin tumor cells and CTCs, the choice of an appropriate suspension medium is all the more relevant, the loss of cell viability leading to erroneous final results [95]. There is an obvious need to develop better suspense solutions for biological particles, both cell-friendlier and more suitable for DEP.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

Liquid Biopsy and Dielectrophoretic Analysis—Complementary Methods in Skin Cancer Monitoring

et al. 2022

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The incidence and prevalence of skin cancers is currently increasing worldwide, with early detection, adequate treatment, and prevention of recurrences being topics of great interest for researchers nowadays. Although tumor biopsy remains the gold standard of diagnosis, this technique cannot be performed in a significant proportion of cases, so that the use of alternative methods with high sensitivity and specificity is becoming increasingly desirable. In this context, liquid biopsy appears to be a feasible solution for the study of cellular and molecular markers relevant to different types of skin cancers. Circulating tumor cells are just one of the components of interest obtained from performing liquid biopsy, and their study by complementary methods, such as dielectrophoresis, could bring additional benefits in terms of characterizing skin tumors and subsequently applying personalized therapy. One purpose of this review is to demonstrate the utility of liquid biopsy primarily in monitoring the most common types of skin tumors: basal cell carcinoma, squamous cell carcinoma, and malign melanoma. In addition, the originality of the article is based on the detailed presentation of the dielectrophoretic analysis method of the most important elements obtained from liquid biopsy, with direct impact on the clinical and therapeutic approach of skin tumors.

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Improving the viability of tissue‐resident stem cells using an organ‐preservation solution

Cited by 7 publications

References 36 publications

Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution

Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution

An efficient polymer cocktail-based transportation method for cartilage tissue, yielding chondrocytes with enhanced hyaline cartilage expression during in vitro culturing

Liquid Biopsy and Dielectrophoretic Analysis—Complementary Methods in Skin Cancer Monitoring

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