Improving the Phenotypic Properties of the <i>Ceratitis capitata</i> (Diptera: Tephritidae) Temperature-Sensitive Lethal Genetic Sexing Strain in Support of Sterile Insect Technique Applications

Porras, Mitzy F.; Meza, José S.; Rajotte, Edwin G.; Bourtzis, Kostas; Cáceres, Carlos

doi:10.1093/jee/toaa220

Cited by 14 publications

(10 citation statements)

References 14 publications

(23 reference statements)

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“…The VIENNA 8 GSS was originally developed at the Insect Pest Control Laboratory of the Joint FAO/IAEA Centre, Seibersdorf, Austria [ 15 ]. In 2015, the strain was refreshed with Valencia local wild population [ 16 ], and as a consequence, the Vienna 8 GSS-Valencia strain was obtained. This strain has two selectable markers which allow male-only production, thus enhancing the efficiency and cost-effectiveness of SIT because: (a) sterile males are not engaged in mating with sterile females [ 13 ]; (b) sterile females are not released and thus do not oviposit in fruits and vegetables; and (c) females are not mass-produced or released, reducing production costs.…”

Section: Production Of Sterile Male Pupaementioning

confidence: 99%

Sterile Insect Technique Programme against Mediterranean Fruit Fly in the Valencian Community (Spain)

Plá

Oteyza²,

Tur

et al. 2021

Insects

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The Mediterranean fruit fly, Ceratitis capitata (Wied.), is an endemic pest in fruit-growing areas of the Spanish Mediterranean coast. In the Valencian Community, it represents a serious problem in the cultivation of citrus and numerous species of fruit, such as peach, cherry, apricot, persimmon, etc. For over 50 years, the Department of Agriculture of Valencia has led, promoted, and carried out a C. capitata control programme to protect crops, especially citrus fruits, because this community is the largest national producer and the leading region for fresh citrus exports in the world. Traditionally, pest control has been based on the use of insecticides. However, a reduction of more than 90% of a target wild population was achieved in the frame of a pilot integrated pest management (IPM) project based on the sterile insect technique (SIT), which was implemented from 2003 to 2006. Based on this successful result, in 2007 the Department of Agriculture of Valencia initiated an area-wide integrated pest management (AW-IPM) programme for the suppression of C. capitata, using the SIT as the primary control method. Complementary activities are implemented periodically in hotspots and during different time periods depending on the pest population dynamics. As a result, there has been a reduction of more than 90% in the use of insecticides by aerial means to control C. capitata, as well as a growth trend in exports of citrus and fresh fruits from the Valencian Community in recent years. This paper provides a historical review of the Valencian programme and briefly describes how technological innovations and decision-making tools have contributed to programme efficiency.

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Section: Production Of Sterile Male Pupaementioning

confidence: 99%

Sterile Insect Technique Programme against Mediterranean Fruit Fly in the Valencian Community (Spain)

Plá

Oteyza²,

Tur

et al. 2021

Insects

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“…The introgression of the tsl-FD into the VIENNA 8 D53 -GSS resulted in a new strain, the “VIENNA 8 D53 -FD GSS”. Characterization of this new GSS confirmed that female larvae develop faster, but also revealed differences in the temperature sensitivity and production profile [ 32 ]. The better biological profile of the new tsl-FD GSS strain will increase the rearing efficiency of C. capitata SIT projects.…”

Section: Main Research Achievements—plant Pestsmentioning

confidence: 89%

The Insect Pest Control Laboratory of the Joint FAO/IAEA Programme: Ten Years (2010–2020) of Research and Development, Achievements and Challenges in Support of the Sterile Insect Technique

Vreysen

Abd-Alla

Bourtzis

et al. 2021

Insects

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The Joint FAO/IAEA Centre (formerly called Division) of Nuclear Techniques in Food and Agriculture was established in 1964 and its accompanying laboratories in 1961. One of its subprograms deals with insect pest control, and has the mandate to develop and implement the sterile insect technique (SIT) for selected key insect pests, with the goal of reducing the use of insecticides, reducing animal and crop losses, protecting the environment, facilitating international trade in agricultural commodities and improving human health. Since its inception, the Insect Pest Control Laboratory (IPCL) (formerly named Entomology Unit) has been implementing research in relation to the development of the SIT package for insect pests of crops, livestock and human health. This paper provides a review of research carried out between 2010 and 2020 at the IPCL. Research on plant pests has focused on the development of genetic sexing strains, characterizing and assessing the performance of these strains (e.g., Ceratitis capitata), elucidation of the taxonomic status of several members of the Bactrocera dorsalis and Anastrepha fraterculus complexes, the use of microbiota as probiotics, genomics, supplements to improve the performance of the reared insects, and the development of the SIT package for fruit fly species such as Bactrocera oleae and Drosophila suzukii. Research on livestock pests has focused on colony maintenance and establishment, tsetse symbionts and pathogens, sex separation, morphology, sterile male quality, radiation biology, mating behavior and transportation and release systems. Research with human disease vectors has focused on the development of genetic sexing strains (Anopheles arabiensis, Aedes aegypti and Aedes albopictus), the development of a more cost-effective larvae and adult rearing system, assessing various aspects of radiation biology, characterizing symbionts and pathogens, studying mating behavior and the development of quality control procedures, and handling and release methods. During the review period, 13 coordinated research projects (CRPs) were completed and six are still being implemented. At the end of each CRP, the results were published in a special issue of a peer-reviewed journal. The review concludes with an overview of future challenges, such as the need to adhere to a phased conditional approach for the implementation of operational SIT programs, the need to make the SIT more cost effective, to respond with demand driven research to solve the problems faced by the operational SIT programs and the use of the SIT to address a multitude of exotic species that are being introduced, due to globalization, and established in areas where they could not survive before, due to climate change.

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“…The experiment was conducted using two medfly strains: one stablished from wild individuals reared under laboratory conditions, and the second one with the Fast Development strain (Vienna 8D53-FD GSS, hereafter referred to as Vienna 8 FD-GSS) (Porras et al, 2020 ). The wild-type colony came from field-collected C. capitata pupae obtained from infested mandarin orange ( Citrus reticulada and C. unshiu ) collected from an experimental citrus orchard located in Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) in October of 2018.…”

Section: Methodsmentioning

confidence: 99%

“…The Vienna 8 fast development strain (Vienna 8D53-FD GSS) is a new GSS originating from the Vienna 8 temperature-sensitive lethal GSS that was developed by FAO/IAEA at the Insect Pest Control Laboratory (IPCL) in Seibersdorf, Austria. This novel strain, which combines the temperature sensitive lethal (tsl) and the white pupae (wp) genes as selectable markers, shows faster development in females during the larval stage and increased temperature sensitivity compared with the regular Vienna 8 strain (Porras et al, 2020 ). The introgression of this novel wp tsl FD combined trait into the Vienna 8D53 GSS, resulted in a novel Vienna 8D53-FD GSS, where females showed differences in the thermal sensibility, larval development speed, and productivity profiles (Porras et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…This novel strain, which combines the temperature sensitive lethal (tsl) and the white pupae (wp) genes as selectable markers, shows faster development in females during the larval stage and increased temperature sensitivity compared with the regular Vienna 8 strain (Porras et al, 2020 ). The introgression of this novel wp tsl FD combined trait into the Vienna 8D53 GSS, resulted in a novel Vienna 8D53-FD GSS, where females showed differences in the thermal sensibility, larval development speed, and productivity profiles (Porras et al, 2020 ). Currently, it is under small-scale laboratory tests and could be a potential candidate to be implemented in mass-rearing conditions in the SIT program in the Valencian Community.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of the Gut Bacteriome During a Laboratory Adaptation Process of the Mediterranean Fruit Fly, Ceratitis capitata

Mokhtar

Catalá-Oltra²,

Stathopoulou

et al. 2022

Front. Microbiol.

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Laboratory adaptation process used in sterile insect technique (SIT) programs can exert a significant impact on the insect-gut microbiome relationship, which may negatively impact the quality and performance of the fly. In the present study, changes in the gut microbiota that occur through laboratory adaptation of two Ceratitis capitata populations were investigated: Vienna 8 genetic sexing strain (GSS), a long-established control line, and a wild population recently introduced to laboratory conditions. The bacterial profiles were studied for both strains using amplicon sequencing of the 16S rRNA V3-V4 hypervariable region in larvae and in the gastrointestinal tract of teneral (1 day) and adults (5 and 15 days) reared under laboratory conditions for 14 generations (F0–F13). Findings demonstrated the development of distinct bacterial communities across the generations with differences in the bacterial composition, suggesting a strong impact of laboratory adaptation on the fly bacteriome. Moreover, different bacterial profiles were observed between wild and Vienna 8 FD-GSS displaying different patterns between the developmental stages. Proteobacteria, mainly members of the Enterobacteriaceae family, represented the major component of the bacterial community followed by Firmicutes (mainly in Vienna 8 FD-GSS adults) and Chlamydiae. The distribution of these communities is dynamic across the generations and seems to be strain- and age-specific. In the Vienna 8 FD-GSS population, Providencia exhibited high relative abundance in the first three generations and decreased significantly later, while Klebsiella was relatively stable. In the wild population, Klebsiella was dominant across most of the generations, indicating that the wild population was more resistant to artificial rearing conditions compared with the Vienna 8 FD-GSS colony. Analysis of the core bacteriome revealed the presence of nine shared taxa between most of the examined medfly samples including Klebsiella, Providencia, Pantoea, and Pseudomonas. In addition, the operational taxonomic unit co-occurrence and mutual exclusion networks of the wild population indicated that most of the interactions were classified as co-presence, while in the Vienna 8 FD-GSS population, the number of mutual exclusions and co-presence interactions was equally distributed. Obtained results provided a thorough study of the dynamics of gut-associated bacteria during the laboratory adaptation of different Ceratitis capitata populations, serving as guidance for the design of colonization protocols, improving the effectiveness of artificial rearing and the SIT application.

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Improving the Phenotypic Properties of the Ceratitis capitata (Diptera: Tephritidae) Temperature-Sensitive Lethal Genetic Sexing Strain in Support of Sterile Insect Technique Applications

Cited by 14 publications

References 14 publications

Sterile Insect Technique Programme against Mediterranean Fruit Fly in the Valencian Community (Spain)

Sterile Insect Technique Programme against Mediterranean Fruit Fly in the Valencian Community (Spain)

The Insect Pest Control Laboratory of the Joint FAO/IAEA Programme: Ten Years (2010–2020) of Research and Development, Achievements and Challenges in Support of the Sterile Insect Technique

Dynamics of the Gut Bacteriome During a Laboratory Adaptation Process of the Mediterranean Fruit Fly, Ceratitis capitata

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