N EW dairy products in the markets have become an urgent need to attract the attention of the consumer and satisfy its passion about the novel products. Adding CO 2 on some dairy products has become modern trend. The production of carbonated probiotic UF-Labneh cheese was tested and evaluated. Lactobacillus acidophilus strain was encapsulated by two different methods (extrusion or spray drying method) in the presence of CO 2 (added 1.5 g solid CO 2 to 100 ml milk). All samples were inoculated with the traditional starter culture, and then divided to five treatments. Control sample inoculated with starter cultures only, while blank sample had no CO 2 gas. Treated sample (T 1 ) was inoculated with 2% free cells of L. acidophilus. The third part (T 2 ) was inoculated with 2% encapsulated L. acidophilus by spray drying method. The fourth part (T 3 ) was inoculated with 2% encapsulated L. acidophilus by extrusion method. All UF-labneh samples were evaluated for microbiological, chemical, rheological and sensory properties during 21 days of cold storage. The data indicated that the counts of L. bulgaricus and S. thermophilus were enhanced during the first 14 days and then a slight decrease was observed at the end of the storage of all treatments. The counts of L. acidophilus were developed during storage period until 14 days, and encapsulated L. acidophilus in T 2 and T 3 had the highest counts. In contrary, T 1 that fortified with free cells had the lowest counts. A little count of mold & yeast was detected in the T 1 , T 2 and T 3 after two weeks. Also, there was a slight decrease in pH values in all treated samples during storage. Blank and control samples had higher pH values compared to treated samples. Additionally, the total solid content of blank samples were lower than the others without significant differences between all samples either fresh or during storage period in fat content. The most texture parameters (Hardness, Cohesiveness, Gumminess and Chewiness) of all labneh samples increased slightly during storage. On the other hand, samples which contained CO 2 and probiotic strains were more accepted. Adding CO 2 and probiotic bacteria to the labneh gave it a new desirable taste not familiar before to consumers.