Improving sensitivity for serodiagnosis of tuberculosis using TB16.3-echA1 fusion protein

Khurshid, Sana; Afzal, Madeeha; Khalid, Ruhi; Khan, Imran; Akhtar, Muhammad

doi:10.1016/j.tube.2014.06.006

Cited by 13 publications

(12 citation statements)

References 11 publications

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“…Most serological TB studies have focused on assays using purified antigens, such as bacille Calmette-Guerin (BCG) antigen [9,10] or other single antigens [11], but increasingly the recommended approach is to use a combination of different antigens [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Serodiagnosis ofMycobacterium abscessuscomplex infection in cystic fibrosis

et al. 2015

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Early signs of pulmonary disease with Mycobacterium abscessus complex (MABSC) can be missed in patients with cystic fibrosis (CF). A serological method could help stratify patients according to risk. The objective of this study was to test the diagnostic accuracy of a novel method for investigating IgG activity against MABSC.A prospective study of all patients attending the Copenhagen CF Centre was conducted by culturing for MABSC during a 22-month period and then screening patients with an anti-MABSC IgG ELISA. Culturepositive patients had stored serum examined for antibody kinetics before and after culture conversion.307 patients had 3480 respiratory samples cultured and were then tested with the anti-MABSC IgG ELISA. Patients with MABSC pulmonary disease had median anti-MABSC IgG levels six-fold higher than patients with no history of infection (434 versus 64 ELISA units; p<0.001). The test sensitivity was 95% (95% CI 74-99%) and the specificity was 73% (95% CI 67-78%). A diagnostic algorithm was constructed to stratify patients according to risk.The test accurately identified patients with pulmonary disease caused by MABSC and was suited to be used as a complement to mycobacterial culture. @ERSpublications Measuring antibodies against Mycobacterium abscessus can identify patients who harbour this emerging threat to health

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Section: Introductionmentioning

confidence: 99%

Serodiagnosis ofMycobacterium abscessuscomplex infection in cystic fibrosis

et al. 2015

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“…Genes encoding immunodominant antigens have often been tandemly linked to encode a single polyprotein (Hoff et al, 2007;Houghton et al, 2002;Khurshid et al, 2014). Fusion proteins consisting of epitopes from two or more antigens are likely to offer a cheaper and more reliable serodiagnosis.…”

Section: Introductionmentioning

confidence: 99%

Fusion of selected regions of mycobacterial antigens for enhancing sensitivity in serodiagnosis of tuberculosis

Afzal

Khurshid

Khalid

et al. 2015

Journal of Microbiological Methods

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“… 37 , 38 This approach has been used successfully for the mycobacterial antigens ESAT-6 and CFP-10, which were fused together in order to significantly increase T-cell activation, IFN-γ production and diagnostic sensitivity. 38 , 39 , 40 By virtue of bioinformatics analysis, six epitopes of Rv0310c and three epitopes of Rv1255c were chosen for further research. It is necessary to evaluate the immunogenicity and immunoreactivity of these fragments before using them in a diagnostic application.…”

Section: Discussionmentioning

confidence: 99%

“… 30 Fusion protein epitopes, such as 38F, 64F, TB16.3-echA1, tn2FbpC1-tnPstS1 and tnHSP-tn1FbpC1, showed improved diagnostic ability for different TB patients. 37 , 38 , 40 Other protein chips or protein combinations also exhibited favorable diagnostic ability for pulmonary and extrapulmonary TB patients. 43 , 44 , 45 During our combination analysis, a sample found to be positive for any assay was considered to be positive, and the best diagnostic performance that was obtained in the SPPT group was the assembly of the two antigens Rv0310c-E and Rv3425; use of three or more antigens would reduce the diagnostic ability.…”

Section: Discussionmentioning

confidence: 99%

Antigens Rv0310c and Rv1255c are promising novel biomarkers for the diagnosis of Mycobacterium tuberculosis infection

Luo

Zhu

Yue

et al. 2017

Emerging Microbes & Infections

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This study aimed to identify novel immunogenic epitopes from Mycobacterium tuberculosis (MTB) that could be used in tuberculosis (TB) diagnostics. To determine the diagnostic potential of mycobacterial antigens in serodiagnosis of TB, 256 patients were enrolled in a study and divided into two groups: 126 smear-positive pulmonary TB patients (SPPT) and 130 smear-negative pulmonary TB patients (SNPT); 152 bacillus Calmette-Guerin (BCG)-vaccinated healthy people were used as a control. Murine results showed that antigens Rv0310c-E from RD 8 and Rv1255c-E from RD 10 were strongly immunogenic to Th1 cells and induced a great humoral response. Receiver operating characteristic analysis indicated that Rv0310c-E (area under the curve (AUC): 0.800) and Rv1255c-E (AUC: 0.808) performed better than ESAT-6 (AUC: 0.665) and CFP-10 (AUC: 0.623) proteins but were comparable with Rv3425 (AUC: 0.788) protein in a human serum IgG analysis. Rv0310c-E demonstrated the highest diagnostic ability for the SPPT group (Youden index: 0.5602, sensitivity: 69.84%, specificity: 86.18%), while Rv1255c-E demonstrated the highest diagnostic ability for the SNPT group (Youden index: 0.5674, sensitivity: 73.84%, specificity: 82.89%). In addition, combination analysis found that antigen Rv0310c-E, coupled with the Rv3425 protein (Youden index: 0.6098, sensitivity: 87.30%, specificity: 73.68%) had the strongest performance for TB diagnostics of the SPPT group, and the single antigen Rv1255c-E was strongest for the SNPT group. These results suggest that antigens Rv0310c-E and Rv1255c-E are potential antigens for TB serodiagnostic tests, which may facilitate detection of MTB in smear-negative and smear-positive patients.

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Improving sensitivity for serodiagnosis of tuberculosis using TB16.3-echA1 fusion protein

Cited by 13 publications

References 11 publications

Serodiagnosis ofMycobacterium abscessuscomplex infection in cystic fibrosis

Serodiagnosis ofMycobacterium abscessuscomplex infection in cystic fibrosis

Fusion of selected regions of mycobacterial antigens for enhancing sensitivity in serodiagnosis of tuberculosis

Antigens Rv0310c and Rv1255c are promising novel biomarkers for the diagnosis of Mycobacterium tuberculosis infection

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