Serodiagnosis of<i>Mycobacterium abscessus</i>complex infection in cystic fibrosis

Qvist, Tavs; Pressler, T.; Taylor-Robinson, David; Katzenstein, Terese L.; Høiby, Niels

doi:10.1183/09031936.00011815

Cited by 32 publications

(41 citation statements)

References 36 publications

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“…The dotted horizontal line is at 125 ELISA Units (test positive threshold). Reproduced from with permission from the authors.…”

Section: Why Early Diagnosis Is Importantmentioning

confidence: 99%

“…Although, some have suggested that the majority of patients with CF and NTM are only transiently culture positive , this concept of airway colonization by NTM has never been tested rigorously . Our finding that MABSC exhibits biofilm growth and induces a humoral immune response , points more toward periods of latent infection than actual transient colonization. In patients fulfilling ATS criteria for pulmonary disease and where other competing causes have been ruled out, hesitation should be avoided.…”

Section: Eradicating Mabscmentioning

confidence: 99%

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The importance of early diagnosis of Mycobacterium abscessus complex in patients with cystic fibrosis

Ravnholt

Kolpen

Skov

et al. 2018

APMIS

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Mycobacterium abscessus complex can cause severe lung infections and has proven to be a serious threat to patients with cystic fibrosis and a challenge for clinicians due to difficulties in timely diagnosis and complex multidrug treatment regimes. Mycobacterial culture is the gold standard for diagnosis, but in most cystic fibrosis centers it is performed less frequently than culture for other pathogens. Consensus today recommends just one annual mycobacterial culture for asymptomatic patients with cystic fibrosis, a strategy likely to lead to diagnostic delays. Postponement of diagnosis might be the deciding factor in whether an early colonization turns into chronic infection. This review highlights the latest developments in knowledge about the pathogenicity and clinical consequences of M. abscessus complex pulmonary disease, addressing the central theme of why pulmonary infection requires early identification and aggressive antibiotic treatment. The window of opportunity, before M. abscessus complex transforms from a mucosal colonizer to a chronic biofilm infection, is where microbial eradication is most likely to be successful, making early diagnosis essential for improved outcomes.

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“…The dotted horizontal line is at 125 ELISA Units (test positive threshold). Reproduced from with permission from the authors.…”

Section: Why Early Diagnosis Is Importantmentioning

confidence: 99%

Section: Eradicating Mabscmentioning

confidence: 99%

The importance of early diagnosis of Mycobacterium abscessus complex in patients with cystic fibrosis

Ravnholt

Kolpen

Skov

et al. 2018

APMIS

Self Cite

View full text Add to dashboard Cite

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“…The dotted horizontal line is the 125 ELISA units (test positive threshold). The dotted vertical line represents the date of first positive culture (reproduced from .…”

Section: Cystic Fibrosis and Biofilm Infection – Pathogenetic And Diamentioning

confidence: 99%

Diagnosis of biofilm infections in cystic fibrosis patients

Høiby

Bjarnsholt

Moser

et al. 2017

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Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex‐mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA‐FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti‐P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.

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“…62 To enhance detection of mycobacterial infection, patients attending the Copenhagen CF Centre were cultured for Mycobacterium abscessus (MABSC) and had an anti-MABSC IgG ELISA performed over 22 months. 63 Patients with MABSC pulmonary disease had median anti-MABSC IgG levels that were sixfold higher than patients with no history of infection (434 vs. 64 ELISA units; P < 0.001); test sensitivity was 95% and specificity was 73%. A study from France showed that chronic administration of azithromycin was protective against NTM infection in adults with CF, and suggested its use as primary prophylaxis.…”

Section: Respiratory Tract Infectionmentioning

confidence: 88%

“…A case report highlighted that non‐tuberculous mycobacteria (NTM), in this instance the Mycobacterium avium complex, may be the first organism of significance isolated from the CF lung . To enhance detection of mycobacterial infection, patients attending the Copenhagen CF Centre were cultured for Mycobacterium abscessus (MABSC) and had an anti‐MABSC IgG ELISA performed over 22 months . Patients with MABSC pulmonary disease had median anti‐MABSC IgG levels that were sixfold higher than patients with no history of infection (434 vs. 64 ELISA units; P < 0.001); test sensitivity was 95% and specificity was 73%.…”

Section: Respiratory Tract Infectionmentioning

confidence: 99%