Improving recombinant MVA immune responses: Potentiation of the immune responses to HIV-1 with MVA and DNA vectors expressing Env and the cytokines IL-12 and IFN-gamma

Abaitua, Fernando; Rodríguez, Juan; Garzón, Ana; Rodrı́guez, Dolores; Esteban, Mariano

doi:10.1016/j.virusres.2005.08.008

Cited by 45 publications

(24 citation statements)

References 40 publications

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“…Ad vectors also have an adjuvant effect [235], which may be further potentiated by co-expressing cytokines such as GMCSF, IL-2 and IL-12 [236]. Ad5 may be suitable for use in small ruminants since these animals have hardly any pre-existing immunity to the vector as has been observed in humans [237].…”

Section: Disease Controlmentioning

confidence: 99%

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

Kumar

Maherchandani

Kashyap

et al. 2014

Viruses

180

168

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Peste des petits ruminants (PPR) is caused by a Morbillivirus that belongs to the family Paramyxoviridae. PPR is an acute, highly contagious and fatal disease primarily affecting goats and sheep, whereas cattle undergo sub-clinical infection. With morbidity and mortality rates that can be as high as 90%, PPR is classified as an OIE (Office International des Epizooties)-listed disease. Considering the importance of sheep and goats in the livelihood of the poor and marginal farmers in Africa and South Asia, PPR is an important concern for food security and poverty alleviation. PPR virus (PPRV) and rinderpest virus (RPV) are closely related Morbilliviruses. Rinderpest has been globally eradicated by mass vaccination. Though a live attenuated vaccine is available against PPR for immunoprophylaxis, due to its instability in subtropical climate (thermo-sensitivity), unavailability of required doses and insufficient coverage (herd immunity), the disease control program has not been a great success. Further, emerging evidence of poor cross neutralization between vaccine strain and PPRV strains currently circulating in the field has raised concerns about the protective efficacy of the existing PPR vaccines. This review summarizes the recent advancement in PPRV replication, its pathogenesis, immune response to vaccine and disease control. Attempts have also been made to highlight the current trends in understanding the host susceptibility and resistance to PPR.

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Section: Disease Controlmentioning

confidence: 99%

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

Kumar

Maherchandani

Kashyap

et al. 2014

Viruses

180

168

View full text Add to dashboard Cite

show abstract

“…Cytokines are the most important mediators of the immune system and they exert their effect as arbitrators between innate and adaptive immune system [11]. Several studies have shown that cytokine expressing vectors, such as GM-CSF expressing vector, can act as adjuvant and enhance the immunogenicity of DNA vaccines [4,14,15]. Therefore, based upon studies performed in this field, co-delivery of cytokine gene is a useful strategy to enhance the immunogenicity of DNA vaccine [16,17].…”

Section: Introductionmentioning

confidence: 99%

ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses

et al. 2011

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“…Using VV as a live recombinant vaccine, many groups have demonstrated that potent cell-mediated and humoral immunity can be generated against an encoded vaccine Ag (18,19). The capacity to insert large pieces of foreign DNA into VV or other poxvirus vectors has enabled the construction of recombinant vaccinia viruses (rVV) expressing multiple proteins, such as vaccine Ags and costimulatory molecules (20,21), that can enhance cell-mediated and humoral immunity to the encoded Ag or the vaccine vector (22)(23)(24). In particular, cytokines known to have antiviral activity such as IFN-␥, or those that influence the differentiation or activation of cell subsets involved in the immune response such as IL-4, have been found to significantly modulate the immune response (24,25 ) mice (8 -11 wk old) were kindly provided by Sue Fordham at The Canberra Hospital.…”

mentioning

confidence: 99%

Differential Effects of the Type I Interferons α4, β, and ε on Antiviral Activity and Vaccine Efficacy

Day

Ramshaw

Ramsay

et al. 2008

The Journal of Immunology

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The type I IFNs exert a range of activities that include antiviral, antiproliferative, and immunomodulatory effects. To study this further, we have constructed recombinant vaccinia viruses expressing HIV or hemagglutinin (HA) Ags along with murine type I IFNs, IFN-α4 (HA-VV-IFN-α4), IFN-β (HA-VV-IFN-β), or IFN-ε (HIV-VV-IFN-ε), a recently discovered member of this family. Our aims were to characterize IFN-ε functionality as a type I IFN and also to study the biological properties of these factors toward the development of safer and more effective vector-based vaccines. HIV-VV-IFN-ε and HA-VV-IFN-β grew to lower titers than did their parental controls in murine cell lines. In vivo, however, HIV-VV-IFN-ε growth was not attenuated, while IFN-β demonstrated potent local antiviral activity with no replication of HA-VV-IFN-β detected. Flow cytofluorometric analysis of B lymphocytes incubated with virally encoded IFN-ε showed up-regulation of activation markers CD69 and CD86, while RT-PCR of IFN-ε-treated cells revealed that gene expression levels of antiviral proteins were elevated, indicating the induction of an antiviral state. The use of these constructs in a poxvirus prime-boost immunization regime led to robust humoral and cellular immune responses against the encoded Ags, despite the lack of replication in the case of HA-VV-IFN-β. Thus, coexpression of these factors may be beneficial in the design of safer vector-based vaccines. Our data also indicate that while IFN-ε exhibits certain biological traits similar to other type I IFNs, it may also have a specific role in mucosal immune regulation that is quite distinct.

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Improving recombinant MVA immune responses: Potentiation of the immune responses to HIV-1 with MVA and DNA vectors expressing Env and the cytokines IL-12 and IFN-gamma

Cited by 45 publications

References 40 publications

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses

Differential Effects of the Type I Interferons α4, β, and ε on Antiviral Activity and Vaccine Efficacy

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