ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses

Mahdavi, Mehdi; Ebtekar, Massoumeh; Khorshid, Hamid Reza Khorram; Azadmanesh, Kayhan; Hartoonian, Christine; Hassan, Zuhair Mohammad

doi:10.1016/j.imlet.2011.05.005

Cited by 27 publications

(21 citation statements)

References 37 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 By definition, the successful adjuvant should be able to effectively enhance both cellular and humoral immune responses as well as remove or control viral and intracellular pathogen infections. 6 To this end, the use of modified naloxone or a mixture of naloxone with a Th2 polarizing adjuvant may be a useful strategy to achieve a suitable adjuvant. Although a well-defined adjuvant for human immunization, being able to trigger humoral immune response (Th2), alum fails to induce the cellular immune response (Th1) which is important for immune responses against a variety of intracellular pathogens.…”

Section: Introductionmentioning

confidence: 99%

Naloxone/alum mixture a potent adjuvant for HIV-1 vaccine: induction of cellular and poly-isotypic humoral immune responses

Farahani

Aghasadeghi

Memarnejadian

et al. 2016

Pathogens and Global Health

Self Cite

View full text Add to dashboard Cite

In the present study we used a fusion peptide from HIV-1 p24 and Nef as vaccine model and adjuvant activity of Naloxone/alum mixture was evaluated in a peptide vaccine model. HIV-1 p24-Nef fusion peptide was synthesized. Female BALB/c mice were divided into five groups. The first group immunized subcutaneously with the p24-Nef fusion peptide adjuvanted with Naloxone/alum mixture and boosted with same protocol. The second was immunized with fusion peptide adjuvanted in alum. The control groups were injected with NLX (Group 3), Alum (Group 4), or PBS (Groups 5) under the same conditions. To determine the type of induced immune response, sera and splenocytes were analyzed by commercial ELISA method for total IgG and isotypes and cytokine secretion (IL-4 & IFN-γ), respectively. We have also used the ELISPOT assay to monitor changes in the frequency of IFN-γ-producing T cells. The proliferation of T cells was assessed using Brdu method and T-cell cytotoxicity was assessed with CFSE method. Immunization of mice with HIV-1 p24-Nef fusion peptide formulated in Naloxone/ alum mixture significantly increased lymphocyte proliferation and shifted cytokine responses toward Th1 profile compared to all other groups. Analysis of humoral immune responses revealed that administration of HIV-1 p24-Nef fusion peptide with Naloxone/alum mixture significantly increased specific IgG responses and also increased IgG1,IgG2a, IgG2b, IgG3, and IgM vs. alum-adjuvanted vaccine groups. Naloxone/alum mixture as an adjuvant could improve cellular and humoral immune response for HIV vaccine model and this adjuvant maybe useful for HIV vaccine model in human clinical trial.

show abstract

Section: Introductionmentioning

confidence: 99%

Naloxone/alum mixture a potent adjuvant for HIV-1 vaccine: induction of cellular and poly-isotypic humoral immune responses

Farahani

Aghasadeghi

Memarnejadian

et al. 2016

Pathogens and Global Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, co-immunization with GM-CSF resulted in a higher level of total IgG, comprising approximately equal levels of both specific IgG1 and IgG2a subtypes. Taken together, the results suggested that GM-CSF is able to induce long term memory for the HIV-1 P24-Nef vaccine candidate [58]. Recent studies have used DNA/protein or DNA/Adenovector regimens for HIV immunization.…”

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 74%

How can Improve DNA Vaccine Modalities as a Therapeutic Approach against HIV Infections?

Habibzadeh¹

2015

J AIDS Clin Res

View full text Add to dashboard Cite

“…Considering this fact that each isotype of antibodies has unique function in inducing the humoral immune response, the production of poly-isotypic humoral immune response show that FliC could stimulate humoral immune response with excellent bioactivity and this argument is proved in passive and active immunotherapy of P. aeruginosa infections (13,17) . In our previous study, we proved that immunization with anti r-fla-A IgG by increasing the phagocytosis and bacterial immobilization could protect burned mice against lethal P. aeruginosa infections (75% protection compared with 16.6% in the control IgG) (17).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is important to develop non-live vaccine formulations capable of generating T helper 1 (Th1) responses in humans (12). There is a strong correlation between innate and adaptive immune responses, so strong innate immune response stimulation could induced strong adaptive immune responses (13). Recent studies have shown that the flagellin as a vaccine candidate or an immunologic adjuvant could improve the vaccine efficacy against P. aeruginosa infections (14,15).…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa Recombinant Flagellin Induced Poly-Isotypic Humoral Immune Responses in the Balb/C Mice

Shajiei

Malal

Shahabi

et al. 2013

Jundishapur J Microbiol

Self Cite

View full text Add to dashboard Cite

Background:Pseudomonas aeruginosa is an opportunistic pathogen that infects people with immunocompromised defenses like neutropenic, burned, hospitalized, and cystic fibrosis (CF) patients. Objectives:The main aim of this study was to explore the possibility of the recombinant type A flagellin (r-fla-A) in combination of Montanide ISA 70 as a candidate vaccine to promote the humoral and cellular immune responses against r-fla-A. Materials and Methods: Recombinant flagellin was prepared in Montanide ISA 70 adjuvant; Mice were divided into two groups one of six. The lymphocyte proliferation assay was performed with Brdu/ELISA and IL-4 and IFN-γ cytokine level assay was carried out to determine the pattern of immune response (Th1 vs. Th2). Specific antibody responses were measured with an optimized in direct ELISA and finally different isotype-specific antibodies including IgG1, IgG2a, IgG2b, IgG3, and IgM was measured with ELISA. Results: Immunized mice with adjuvanted flagellin showed a considerably increased lymphocyte proliferation compared with the control group (P = 0.004). High level of IL-4 and IFN-γ secretion was observed in immunized mice compared with the control group (P = 0.003 and P = 0.006, respectively) with Th1 profile. In addition to the strong antibody-mediated immune response, we found that immunization of mice with r-fla-A induces specific IgG1, IgG2a, IgG2b, IgG3 and IgM antibodies that indicates a statistically significant difference with the control group (P = 0.003, P = 0.004, P = 0.004, P = 0.006 and P = 0.004 respectively). Conclusions: Our results demonstrated that r-fla-A could induce cellular and humoral immune response as proper stimulant of polyisotypic humoral responses.

show abstract

ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses

Cited by 27 publications

References 37 publications

Naloxone/alum mixture a potent adjuvant for HIV-1 vaccine: induction of cellular and poly-isotypic humoral immune responses

Naloxone/alum mixture a potent adjuvant for HIV-1 vaccine: induction of cellular and poly-isotypic humoral immune responses

How can Improve DNA Vaccine Modalities as a Therapeutic Approach against HIV Infections?

Pseudomonas aeruginosa Recombinant Flagellin Induced Poly-Isotypic Humoral Immune Responses in the Balb/C Mice

Contact Info

Product

Resources

About