Improving peptide quantification in chitosan nanoparticles

Ribeiro, Maycon Carvalho; Corrêa, Viviane Lopes Rocha; Silva, Francenya Kelley Lopes da; Neto, Jerônimo Raimundo de Oliveira; Casas, Ariadine Amorim; Menezes, Liliana Borges de; Amaral, André Corrêa

doi:10.1016/j.ijbiomac.2018.07.119

Cited by 10 publications

(2 citation statements)

References 27 publications

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“…Nanoparticles conjugated to peptides have been known to drastically increase in size, indicating that peptide surface chemistry can impact overall particle diameter. 39,40 The larger diameter of these protein-derived affinity CDs may have an additional benefit over other quantum dots or small molecules when targeting CD71 because receptormediated endocytosis requires a membrane wrapping process triggered by an affinity ligand binding to the receptor. However, thermodynamics limit the size of the particle that can efficiently prompt membrane wrapping processes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…We hypothesize that some amount of the large size we observe in the TEM is due to the presence of the original protein’s peptides retained in the structure and on the surface of the CDs. Nanoparticles conjugated to peptides have been known to drastically increase in size, indicating that peptide surface chemistry can impact overall particle diameter. , …”

Section: Results and Discussionmentioning

confidence: 99%

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Cancer Cell Targeting Via Selective Transferrin Receptor Labeling Using Protein-Derived Carbon Dots

Strickland,

Jorns,

Fourroux

et al. 2024

ACS Omega

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Carbon dot (CD) nanoparticles offer tremendous advantages as fluorescent probes in bioimaging and biosensing; however, they lack specific affinity for biomolecules, limiting their practical applications in selective targeting. Nanoparticles with intrinsic affinity for a target have applications in imaging, cytometry, therapeutics, etc. Toward that end, we report the transferrin receptor (CD71) targeting CDs, synthesized for the first time. The formation of these particles is truly groundbreaking, as direct tuning of nanoparticle affinity was achieved by simple and careful precursor selection of a protein, which has the targeting characteristic of interest. We hypothesized that the retention of the original protein's peptides on the nanoparticle surface provides the CDs with some of the function of the precursor protein, enabling selective binding to the protein's receptor. This was confirmed with FTIR (Fourier transform infrared) data and subsequent affinitybased cell assays. These transferrin (Tf)-derived CDs have been shown to possess an affinity for CD71, a cancer biomarker that is ubiquitously expressed in nearly every cancer cell line due to its central role mediating the uptake of cellular iron. The CDs were tested using the human leukemia cell line HL60 and demonstrated the selective targeting of CD71 and specific triggering of transferrin-mediated endocytosis via clathrin-coated pits. The particle characterization results reflect a carbon-based nanoparticle with bright violet fluorescence and 7.9% quantum yield in aqueous solution. These unpresented CDs proved to retain the functional properties of the precursor protein. Indicating that this process can be repeated for other disease biomarkers for applications ranging from biosensing and diagnostic bioimaging to targeted therapeutics.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%