Anti-inflammatory and anti-gouty-arthritic effect of free Ginsenoside Rb1 and nano Ginsenoside Rb1 against MSU induced gouty arthritis in experimental animals

Liu, Yuan; Zhu, Haiyang; Zhou, Wei; Ye, Qing

doi:10.1016/j.cbi.2020.109285

Cited by 24 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous ginsenosides and ginseng saponins have been demonstrated to inhibit NLRP3 inflammasome activation, resulting in the suppression of inflammatory responses and amelioration of disease conditions. Rb1, Rg1, Rg2, Rg3, Rg5, Rd, Re, Rh1, 25-OCH 3 -PPD, and compound K (CK) in Panax ginseng inhibit NLRP3 inflammasome stimulation, leading to suppressed inflammatory responses and multiple disease conditions, such as obesity, gouty arthritis, atherosclerosis , non-alcoholic fatty liver disease, liver injury, hyperlipidemia, type I diabetes, myocardial hypertrophy and dysfunction, cerebral ischemia and reperfusion injury, colitis, hepatic fibrosis, sepsis, neuronal damage, kidney injury, and cognitive deficits [ [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] ]. Korean red ginseng (KRG) extract and KRG saponin fraction in Panax ginseng also inhibited inflammatory responses by inhibiting NLRP3 inflammasome activation in macrophages, monocytes, sepsis mice, and aging mice [ 65 , 74 ].…”

Section: Inhibitory Role Of Ginseng In Inflammasome Activationmentioning

confidence: 99%

“… Target Ginseng Components Models Ref. NLRP3 Panax ginseng Rb1 3T3-L1, adipose tissue [ 55 ] Gouty arthritic rats [ 56 ] Rg1 NAFLD mice [ 57 ] Liver injury mice [ 58 , 59 ] BNCC337685, diabetic nephropathy rats [ 60 ] Diabetic mice [ 61 ] Cardiomyocytes, myocardial injury mice [ 62 ] Rg2 KC, NAFLD mice [ 63 ] Rg3 RAW264.7, sepsis mice [ 64 ] BMDMs, THP-1, sepsis mice [ 65 ] HK-2, kidney injury mice [ 66 ] AC16, HCM, cardiomyocytes, myocardial hypertrophy rats [ 67 ] Rg5 Diabetic nephropathy mice [ 68 ] Rd THP-1, colitis mice [ 69 ] Cerebral IRI mice [ 70 ] Re Memory impairment mice [ 71 ] Rh1 BMDMs, THP-1, sepsis mice [ 65 ] KC, NAFLD mice [ 63 ] 25-OCH 3 -PPD HSC-T6, hepatic fibrosis mice …”

Section: Inhibitory Role Of Ginseng In Inflammasome Activationmentioning

confidence: 99%

See 1 more Smart Citation

Potential benefits of ginseng against COVID-19 by targeting inflammasomes

2022

Journal of Ginseng Research

View full text Add to dashboard Cite

Section: Inhibitory Role Of Ginseng In Inflammasome Activationmentioning

confidence: 99%

Section: Inhibitory Role Of Ginseng In Inflammasome Activationmentioning

confidence: 99%

Potential benefits of ginseng against COVID-19 by targeting inflammasomes

2022

Journal of Ginseng Research

View full text Add to dashboard Cite

“…Gout rat were randomly divided into the Sham group, model group, and FLA group. At the set time points (2,4,6,12, and 24 h), the heart, liver, spleen, lung, kidney, and ankle of anesthetized rat were taken. And use IVIS Spectrum, PerkinElmer system to record the uorescence distribution (n = 3/group).…”

Section: Target Evaluationmentioning

confidence: 99%

“…Several nano agents were directly applied for uric acid reduction without loading additional antiphlogistic durgs, but the in ammatory problems were hardly solved [10,11]. The limited e ciency of these nanomedicines might be attributed to their therapeutic strategies, which only focused on reducing MSU [12]. During the acute attack of gout ares, it is not suggested to use uric acid lowering drugs.…”

Section: Introductionmentioning

confidence: 99%

Folic Acid-modified Lysozyme-protected Gold Nanoclusters as Anti-inflammatory Nanomedicine for Gout Flares

Sun

Zhuang

Wen

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundGout normally occurs when excess urate crystals accumulate in the joints that induce inflammation. The inflammations further result in gout flares, causing serious pain. Some anti-inflammatory drugs can relieve pain, but they have significantly toxic and side effects. Nanomedicines offer opportunities to reduce various side effects but are mostly based on the delivery of additional organic drugs, which adds complexity and may cause other health problems including the bioaccumulation risk. We developed folic acid (FA)-modified lysozyme (Lys)-protected gold nanoclusters (AuNCs) (i. e., FLA) by a one-pot method, and FLA is developed as an anti-inflammatory drug in the treatment of acute attacks of gout flares at high monosodium urate (MSU) levels. MethodsThe morphological changes of the cells caused by FLA were studied by Transmission electron microscopy (TEM) and fluorescence microscopy. Western blotting was conducted to understand the inflammation factors. AutoDock Vina was used for molecular docking of FLA to evaluate the binding mode of the ligand and the interaction in the active site.ResultsThis nanomedicine significantly reduced the inflammation of gout flares. In addition, the oxidative stress level was found to decrease to normal values by FLA based on the evaluation of reactive oxygen species (ROS). By injecting FLA into the gout rat model, inflammation was remarkably reduced during gout attacks, and cartilage destruction was significantly relieved. The biosafety of this nanomedicine is confirmed by insignificant cytotoxicity and no bioaccumulation-induced organ change.ConclusionCompared with traditional nano-delivery systems, this organic drug-free nanomedicine exhibits exciting therapeutic efficiency against gout flares, which will have considerable prospects in the clinic.

show abstract

“…Ginsenoside Rb1 (known as one of the main bioactive components of ginseng ) is the major constituent screened from Weipixiao ( Zeng et al, 2016 ), a Chinese herbal prescription showing therapeutic effect against GPL, as revealed by previous clinical and animal studies ( He et al, 2017 ; Zhang, 2017 ; Zeng et al, 2018 ). Previously, GRb1 has been proved to be a safe extraction and possess antineoplastic ( Liu et al, 2017 ), antioxidative ( Miao et al, 2017 ), anti-inflammatory ( Liu et al, 2020 ) and anti-angiogenesis ( Lu et al, 2017 ) bioactivities. In addition, an earlier study identified the gastroprotective activity of GRb1 in an ethanol-induced gastric lesion model, and demonstrated that the anti-ulcer effect is produced through an increase in mucus secretion ( Jeong et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rb1 Lessens Gastric Precancerous Lesions by Interfering With β-Catenin/TCF4 Interaction

Zeng

Zhao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Background: Seeking novel and effective therapies for gastric precancerous lesions (GPL) is crucial to reducing the incidence of gastric cancer. Ginsenoside Rb1 (GRb1) is a major ginsenoside in ginseng and has been proved to possess multiple bioactivities. However, whether GRb1 could protect against GPL and the underlying mechanisms have not been explored.Methods: We evaluated the effects of GRb1 on gastric precancerous lesions in rats on macroscopic, microscopic and ultramicroscopic levels. Then, an antibody array was employed to screen differential expression proteins (DEPs). Validation for the targeting DEP and investigation for the possible mechanism was conducted using immunohistochemistry, qRT-PCR, TUNEL apoptosis assay, immunoprecipitation and immunoblotting.Results: GRb1 was found to reverse intestinal metaplasia and a portion of dysplasia in the MNNG-induced GPL rats. The antibody array assay revealed seven DEPs in GPL rats as compared to control rats (5 DEPs were up-regulated, while two DEPs were down-regulated). Among the DEPs, β-catenin, beta-NGF and FSTL1 were significantly down-regulated after GRb1 administration. Our validation results revealed that enhanced protein expression and nuclear translocation of β-catenin were present in animal GPL samples. In addition, analysis of human gastric specimens demonstrated that β-catenin up-regulation and nuclear translocation were significantly associated with advanced GPL pathology. GRb1 intervention not only decreased protein expression and nuclear translocation of β-catenin, but interfered with β-catenin/TCF4 interaction. Along with this, declined transcriptional and protein expression levels of downstream target genes including c-myc, cyclin D1 and Birc5 were observed in GRb1-treated GPL rats.Conclusion: GRb1 is capable of preventing the occurrence and progression of GPL, which might be contributed by diminishing protein expression and nuclear translocation of β-catenin and interfering with β-catenin/TCF4 interaction.

show abstract

Anti-inflammatory and anti-gouty-arthritic effect of free Ginsenoside Rb1 and nano Ginsenoside Rb1 against MSU induced gouty arthritis in experimental animals

Cited by 24 publications

References 22 publications

Potential benefits of ginseng against COVID-19 by targeting inflammasomes

Potential benefits of ginseng against COVID-19 by targeting inflammasomes

Folic Acid-modified Lysozyme-protected Gold Nanoclusters as Anti-inflammatory Nanomedicine for Gout Flares

Ginsenoside Rb1 Lessens Gastric Precancerous Lesions by Interfering With β-Catenin/TCF4 Interaction

Contact Info

Product

Resources

About