Improving hemocompatibility of decellularized liver scaffold using Custodiol solution

Dias, Marlon Lemos; Paranhos, Bruno Andrade; Ferreira, Juliana Ribeiro Pinheiro; Fonseca, Roberto J. C.; Batista, Cíntia Marina Paz; Martins-Santos, R; Andrade, Cherley Borba Vieira de; Faccioli, Lanuza Alaby Pinheiro; Silva, Alexandre Cerqueira da; Nogueira, Fábio César Sousa; Domont, Gilberto B.; Goldenberg, Regina Coeli dos Santos

doi:10.1016/j.msec.2022.112642

Cited by 5 publications

(7 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some cases, these solutions were used during ex vivo blood perfusion prior to transplantation, such as a blood diluent and such anticoagulant aims. 63 – 69 …”

Section: Bioengineering Approachesmentioning

confidence: 99%

“…In addition, we demonstrated that Custodiol was an efficient tool to improve the hemocompatibility of decellularized liver scaffold. 69 Therefore, organ storage solutions can be important alternatives to promote the preservation and to extend liver scaffold’s lifespan.…”

Section: Bioengineering Approachesmentioning

confidence: 99%

“…In some cases, these solutions were used during ex vivo blood perfusion prior to transplantation, such as a blood diluent and such anticoagulant aims. [63][64][65][66][67][68][69] In this regard, our group opened a new frontier in liver scaffold preparation. We previously showed that Custodiol, a common storage solution, can be used to promote acellular and recellularized liver scaffolds perfusion and storage before orthotopic and heterotopic transplantation.…”

Section: Liver Scaffold Preparation Before Transplantationmentioning

confidence: 99%

See 2 more Smart Citations

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

2022

Self Cite

View full text Add to dashboard Cite

Liver transplantation is the only definitive treatment for many diseases that affect this organ, however, its quantity and viability are reduced. The study of liver scaffolds based on an extracellular matrix is a tissue bioengineering strategy with great application in regenerative medicine. Collectively, recent studies suggest that liver scaffold transplantation may assist in reestablishing hepatic function in preclinical diseased animals, which represents a great potential for application as a treatment for patients with liver disease in the future. This review focuses on useful strategies to promote liver scaffold transplantation and the main open questions about this context. We outline the current knowledge about ex vivo bioengineered liver transplantation, including the surgical techniques, recipient survival time, scaffold preparation before transplantation, and liver disease models. We also highlight the current limitations and future directions regarding in vivo bioengineering techniques.

show abstract

“…In some cases, these solutions were used during ex vivo blood perfusion prior to transplantation, such as a blood diluent and such anticoagulant aims. 63 – 69 …”

Section: Bioengineering Approachesmentioning

confidence: 99%

Section: Bioengineering Approachesmentioning

confidence: 99%

Section: Liver Scaffold Preparation Before Transplantationmentioning

confidence: 99%

See 1 more Smart Citation

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, modifying the surface of natural materials in tissue engineering seems necessary to overcome this challenge. Surface modification is performed to improve cell activity at the material’s surface without changing the tissue’s overall skeletal integrity ( Hsia et al, 2019 ; Li et al, 2022b ; Dias et al, 2022 ). It can enhance re-endothelialization and prevent clot formation within the acellular vasculature.…”

Section: The Role Of Surface Modification In Endothelialization Enhan...mentioning

confidence: 99%

“…Tissue engineering and regenerative medicine, aiming to design, produce, and achieve an efficient and functional organ, play a crucial role in overcoming immunity limitations and transplantation obstacles ( Ahmadi et al, 2022 ; Li et al, 2022a ; Cox-Pridmore et al, 2022 ). Decellularization and recellularization (D&R) of tissues and organs are efficient alternatives to classic organ transplantation ( Dias et al, 2022 ). The purpose of decellularization is to wash the complete cell content of the target organ or tissue to achieve a cell-free extracellular matrix (ECM) scaffold while preserving parenchymal and vascular structures within this scaffold ( Shahraki et al, 2021 ; Uday Chandrika et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Mazloomnejad

Babajani

Kasravi

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Decellularization of tissues and organs has recently become a promising approach in tissue engineering and regenerative medicine to circumvent the challenges of organ donation and complications of transplantations. However, one main obstacle to reaching this goal is acellular vasculature angiogenesis and endothelialization. Achieving an intact and functional vascular structure as a vital pathway for supplying oxygen and nutrients remains the decisive challenge in the decellularization/re-endothelialization procedure. In order to better understand and overcome this issue, complete and appropriate knowledge of endothelialization and its determining variables is required. Decellularization methods and their effectiveness, biological and mechanical characteristics of acellular scaffolds, artificial and biological bioreactors, and their possible applications, extracellular matrix surface modification, and different types of utilized cells are factors affecting endothelialization consequences. This review focuses on the characteristics of endothelialization and how to optimize them, as well as discussing recent developments in the process of re-endothelialization.

show abstract

Resident Liver Stem Cells

Faccioli,

Dias,

Martins-Santos

et al. 2024

Resident Stem Cells and Regenerative Therapy

View full text Add to dashboard Cite

Improving hemocompatibility of decellularized liver scaffold using Custodiol solution

Cited by 5 publications

References 51 publications

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Resident Liver Stem Cells

Contact Info

Product

Resources

About