Improving Flavonoid Extraction from Ginkgo biloba Leaves by Prefermentation Processing

Wang, Jiahong; Cao, Fuliang; Su, Erzheng; Wu, Caie; Zhao, Linguo; Ying, Ruifeng

doi:10.1021/jf400712n

Cited by 44 publications

(36 citation statements)

References 39 publications

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“…Aspergillus niger is one of the most important species in the food industry. It is widely used for the preparation of various enzymes, such as cellulase, hemicellulase, and pectinase . Aspergillus niger is considered to be a safe food strain .…”

Section: Resultsmentioning

confidence: 99%

“…It is widely used for the preparation of various enzymes, such as cellulase, hemicellulase, and pectinase. 15,16,23,24 Aspergillus niger is considered to be a safe food strain. 25 To select an A. niger strain with high hydrolysis ability, a total of 20 A. niger isolates were investigated by observing clear zones using Congo red staining on agar plates containing 0.05% date residues ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Twenty strains of Aspergillus niger ( A. niger ) were used in this research. The Gyx086 and Gyx017 strains were isolated from ancient ginkgo tree soil in Anlu city, China (Wang et al …”

Section: Methodsmentioning

confidence: 99%

“…Date residues with size 60 meshes were supplied by the Shanxi Zhongying Redzao Co., Ltd., China Twenty strains of Aspergillus niger (A. niger) were used in this research. The Gyx086 and Gyx017 strains were isolated from ancient ginkgo tree soil in Anlu city, China (Wang et al 15 ). The other strains, isolated from forestry soil in Thunder Bay, Canada, were stored in the lab.…”

Section: Methodsmentioning

confidence: 99%

“…To facilitate the release of nutrients and / or active substances, most of which are the intracellular materials from plant cells, it is usually necessary and effective to break the cell walls to enhance the yield. 15 The conventional method of extracting date juice in China is to extract the dried jujube powder using water with a solid / liquid ratio at 1:10, 60 °C for 2 h. Then the mixture is divided into two fractions, the date juice and the residues. However, mechanical crushing consumes a large amount of electricity and has a very limited effect in reducing the date size due to the large viscosity of the date powder, resulting from its high sugar content.…”

Section: Introductionmentioning

confidence: 99%

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Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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A low‐cost recycling system for the production of pectinase was built to improve the yield and quality of Muzao date juice and to reduce production costs. An Aspergillus niger strain, Gyx086, was selected from 20 A. niger strains on potato agar plates containing Muzao date residues, and the strain was used to produce an enzyme cocktail with high pectinase activity using Muzao date residues as the medium. The high pectinase activity level was therefore used in the production of Muzao date juice. Under optimized enzymatic hydrolysis conditions, a date juice yield of 92.88% was obtained, which was 11% higher than the control, and was not significantly different from a commercial preparation. Further storage trials indicated that the clarity, viscosity, storage stability, and soluble solid content could also be greatly improved by enzymatic treatment. These results suggested that the cheap experimental preparation could effectively replace the commercial preparation used for the production of date juice. This is the first systematic report detailing the production of Muzao date juice using a cheap and effective pectinase cocktail, which could promote the development of the date juice industry. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Twenty strains of Aspergillus niger ( A. niger ) were used in this research. The Gyx086 and Gyx017 strains were isolated from ancient ginkgo tree soil in Anlu city, China (Wang et al …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

Self Cite

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Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology

Han

Chio²,

et al. 2021

Biofuels Bioprod Bioref

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Ginkgo biloba flavonoids are important natural bioactive compounds with strong physiological effects. To develop an eco‐friendly and effective method for extracting flavonoids from plants, a microbial method involving lignocellulose‐degrading bacterial enzymes was developed to improve flavonoid extraction from Ginkgo biloba leaves. In this study, a newly isolated bacterial strain, Paenarthrobacter sp. S1.3, was employed for the fermentation processing of Ginkgo biloba leaf powder. The enzymatic characteristics of xylanase, CMCase, polygalacturonase, and β‐glucosidase from Paenarthrobacter sp. S1.3 were also investigated. The major fermentation parameters for the microbial extraction of Ginkgo biloba flavonoids were optimized using response surface methodology. Under optimal conditions (30.6 °C, initial pH 7.6, and fermentation time 25 h), a total flavonoid yield of 34.15 ± 0.52 mg g−1 dry weight was obtained, which was 2.11 fold and 1.42 fold higher than the yields obtained from the unfermented and ethanol extraction methods, respectively. The optimized results also suggest that the polygalacturonase and β‐glucosidase might play an important role in flavonoid extraction. Fermentation utilizing lignocellulose‐degrading bacteria was a feasible green approach to improve the extraction of natural compounds from plants. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Characterization and optimization of hydrothermal extraction of quercetin from Quercus leaves using response surface methodology

et al. 2021

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A hydrothermal process using subcritical water and ethanol as an extraction solvent has been studied to extract high-value flavonoid compounds from Quercus leaves. The univariate analysis showed that the maximum quercetin yield was 34.6 mg/g obtained at the liquid-solid ratio of 30:1 ml/g, concentration of ethanol 60% (V/V), extraction temperature of 180 C, and extraction duration of 5 h. Based on the Box-Behnken experiment design, the response surface methodology (RSM) was used to optimize the liquid-to-solid ratio, extraction time, extraction temperature, ethanol concentration, and other operating conditions of hydrothermal extraction of quercetin. The regression equation showed that the maximum yield of quercetin was 36.1 mg/g under the conditions of solvent ethanol concentration of 65% (V/V), liquid-solid ratio of 40:1 ml/g, extraction temperature of 189 C, and extraction duration of 5.5 h. The quercetin yield of 36.1 mg/g obtained by the hydrothermal method was 5.6 times the 6.4 mg/g obtained by the traditional ethanol reflux extraction at the same concentration of ethanol 65% (V/V), liquid-solid ratio of 40:1 ml/g, and extraction duration of 5.5 h, but at the boiling point of the solvent.

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Improving Flavonoid Extraction from Ginkgo biloba Leaves by Prefermentation Processing

Cited by 44 publications

References 39 publications

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology

Characterization and optimization of hydrothermal extraction of quercetin from Quercus leaves using response surface methodology

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