Improving acquisition times of elemental bio-imaging for quadrupole-based LA-ICP-MS

Lear, Jessica; Hare, Dominic J.; Adlard, Paul A.; Finkelstein, David I.; Doble, Philip

doi:10.1039/c1ja10301f

Cited by 69 publications

(70 citation statements)

References 14 publications

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“…Thus, the laser and ICP-MS parameters must be carefully selected for each particular sample to optimise the analytical conditions in terms of sensitivity, spatial resolution, and analysis time. Lear et al [28] have recently investigated the relationship between laser scan speed, MS dwell time, and image resolution for the purpose of speeding up typical total acquisition times for constructing images of mice brain sections. It was observed that the original relative dimensions of the samples are maintained in LA-ICP-MS images if the laser scan speed is equal to the laser spot diameter divided by the scan cycle time in the MS.…”

Section: Optimisation Of the Laser And Icp-ms Parametersmentioning

confidence: 99%

Laser ablation ICP-MS for quantitative biomedical applications

et al. 2012

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LA-ICP-MS allows precise, relatively fast, and spatially resolved measurements of elements and isotope ratios at trace and ultratrace concentration levels with minimal sample preparation. Over the past few years this technique has undergone rapid development, and it has been increasingly applied in many different fields, including biological and medical research. The analysis of essential, toxic, and therapeutic metals, metalloids, and nonmetals in biomedical tissues is a key task in the life sciences today, and LA-ICP-MS has proven to be an excellent complement to the organic MS techniques that are much more commonly employed in the biomedical field. In order to provide an appraisal of the fast progress that is occurring in this field, this review critically describes new developments for LA-ICP-MS as well as the most important applications of LA-ICP-MS, with particular emphasis placed on the quantitative imaging of elements in biological tissues, the analysis of heteroatom-tagged proteins after their separation and purification by gel electrophoresis, and the analysis of proteins that do not naturally have ICP-MS-detectable elements in their structures, thus necessitating the use of labelling strategies.

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Section: Optimisation Of the Laser And Icp-ms Parametersmentioning

confidence: 99%

Laser ablation ICP-MS for quantitative biomedical applications

et al. 2012

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“…However, the maximum feed rate of the laser beam in the xdirection in a line-by-line scan is typically limited by the washout time of the ablation cell and the scan speed of the quadrupole mass analyzer. Currently, the maximum number of pixels that can be recorded per second seems to be in the region of 4Hz [34]. This also means that if one is interested in only one analyte, the dwell time of the quadrupole is adjusted in this case to f=0.25s, whereas the settle times of the quadrupole are not taken into account.…”

Section: General Considerationsmentioning

confidence: 99%

Elemental Bioimaging by Means of Fast Scanning Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry

Wehe

Thyssen

Herdering

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. One of the most common setups for elemental bioimaging, the hyphenation of a laser ablation (LA) system and an inductively coupled plasma mass spectrometer (ICP-MS), was expanded by adding full scan mass spectrometric information as another dimension of information. While most studies deal with the analysis of typically not more than up to 10 isotopes per scan cycle, a fast scanning quadrupole mass analyzer was utilized to record the full mass spectrum of interest in this work. Mass-to-charge ratios from 6 to 250 were observed within one cycle. Besides the xand y-position on the ablated sample and the intensity, the m/z-ratio served as fourth variable for each pixel of the obtained data, closing thereby the gap between Binorganic^and Borganic^mass spectrometric imaging techniques. The benefits of this approach include an improved control of interferences, the discovery of unexpected elemental distributions, the possibility to plot isotopic ratios, and to integrate the intensities of a certain number of mass channels recorded for each isotope, thus virtually increasing sensitivity. The respective data are presented for dried droplets as well as embedded animal and human tissue slices. Limits of detection were calculated and found to be in accordance with counting statistics. A dedicated software macro was developed for data manipulation prior to common evaluation and image creation.

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“…As described by Lear et al 21 two crucial parameters are quadrupole dwell time and laser scan speed.…”

Section: Optimization Of Measurement Conditionsmentioning

confidence: 99%

Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard

Bonta

Lohninger

László

et al. 2014

J. Anal. At. Spectrom.

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LA-ICP-MS has often been applied for the analysis of trace elemental distributions in biological tissues. However, the strong matrix dependence of LA-ICP-MS analyses and highly variable matrix conditions aggravate reliable qualitative distribution analyses and thus for obtaining quantitative information elaborate quantification strategies have to be applied. In this work printed patterns on paper with gold thin-layers as pseudo-internal standard have been proposed as an alternative approach to the commonly used matrix-matched tissue standards. Besides a major reduction of the workload for standard preparation the presented method allows for compensation of instrumental drifts during measurement as well as a reduction of matrix related effects. The developed method has been verified using matrixmatched tissue standards (deviations from the actual metal content less than 5% with relative standard deviations of less than 7%) and applied to platinum imaging on human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs.

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Improving acquisition times of elemental bio-imaging for quadrupole-based LA-ICP-MS

Cited by 69 publications

References 14 publications

Laser ablation ICP-MS for quantitative biomedical applications

Laser ablation ICP-MS for quantitative biomedical applications

Elemental Bioimaging by Means of Fast Scanning Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry

Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard

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