Laser ablation ICP-MS for quantitative biomedical applications

Konz, Ioana; Fernández, Beatriz; Fernández, María Luisa López; Pereiro, Rosario; Sanz‐Medel, Alfredo

doi:10.1007/s00216-012-6023-6

Cited by 114 publications

(76 citation statements)

References 98 publications

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“…In addition, quantitative images can be obtained when using the appropriate standardization approaches. 24,25,26 To date, several reports have described the imaging of nanomaterials in cells, 27,28,29 tissues 30,31,32 and plants. 33 A few of these studies have involved AuNPs, yet all but one 33 have measured AuNPs with core sizes between 13 and 50 nm.…”

Section: Introductionmentioning

confidence: 99%

Quantitative imaging of 2 nm monolayer-protected gold nanoparticle distributions in tissues using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS)

Elci

Yan

Kim

et al. 2016

Analyst

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Functionalized gold nanoparticles (AuNPs) have unique properties that make them important biomedical materials. Optimal use of these materials, though, requires an understanding of their fate in vivo. Here we describe the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to image the biodistributions of AuNPs in tissues from mice intravenously injected with AuNPs. We demonstrate for the first time that the distributions of very small (~ 2 nm core) monolayer-protected AuNPs can be imaged in animal tissues at concentrations in the low parts-per-billion range. Moreover, the LA-ICP-MS images reveal that the monolayer coatings on the injected AuNPs influence their distributions, suggesting that the AuNPs remain intact in vivo and their surface chemistry influences how they interact with different organs. We also demonstrate that quantitative images of the AuNPs can be generated when the appropriate tissue homogenates are chosen for matrix matching. Overall, these results demonstrate the utility of LA-ICP-MS for tracking the fate of biomedically-relevant AuNPs in vivo, facilitating the design of improved AuNP-based therapeutics.

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Section: Introductionmentioning

confidence: 99%

Quantitative imaging of 2 nm monolayer-protected gold nanoparticle distributions in tissues using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS)

Elci

Yan

Kim

et al. 2016

Analyst

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“…Most investigators use matrix-matched in-house standards such as spiked tissue homogenates or agarose gels. Various normalization approaches and quantification strategies are described in the reviews of Konz et al 16 and Hare et al 18 In this study, we investigated the detection of nanoparticle accumulation in histological sections of atherosclerotic plaques via detection of europium with LA-ICP-MS. Moreover, based on calibration series of the Eu-VSOP suspension dropped onto liver tissue sections, we quantified and compared Eu-VSOP with endogenous iron content in aortic roots sections.…”

mentioning

confidence: 99%

LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

Scharlach¹,

Müller²,

Wagner³

et al. 2016

j biomed nanotechnol

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The development of iron oxide nanoparticles for biomedical applications requires accurate histological evaluation. Prussian blue iron staining is widely used but may be unspecific when tissues contain substantial endogenous iron. Here we tested whether microscopy by laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) is sensitive enough to analyze accumulation of very small iron oxide particles (VSOP) doped with europium in tissue sections. For synthesis of VSOP, a fraction of Fe3+ (5 wt%) was replaced by Eu3+, resulting in particles with 0.66 mol% europium relative to iron (Eu-VSOP) but with otherwise similar properties as VSOP. Eu-VSOP or VSOP was intravenously injected into ApoE-/- mice on Western cholesterol diet and accumulated in atherosclerotic plaques of these animals. Prussian blue staining was positive for ApoE-/- mice with particle injection but also for controls. LA-ICP-MS microscopy resulted in sensitive and specific detection of the europium of Eu-VSOP in liver and atherosclerotic plaques. Furthermore, calibration with Eu-VSOP allowed calculation of iron and particle concentrations in tissue sections. The combination of europium-doped iron oxide particles and LA-ICP-MS microscopy provides a new tool for specific and quantitative analysis of particle distribution at the tissue level and allows correlation with other elements such as endogenous iron.

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“…Thus, the methods must be feasible for working with greater and smaller amounts of a sample and must also be in agreement with green chemistry recommendations. Other options with inherent advantages for the determination of halogens in biological samples are direct sampling [136], electrothermal vaporization [204,205], and laser ablation coupled with ICP-MS [103,206,207]. Additionally, although few applications were found for determination of halogens, laser-induced-breakdown spectrometry can be considered a potential detection technique mainly regarding determination of halogens in pharmaceutical products [208] because it allows analysis almost free of a sample preparation step [209,210].…”

Section: Final Considerationsmentioning

confidence: 98%

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

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Fluorine, chlorine, bromine, and iodine have been studied in biological samples and other related matrices owing to the need to understand the biochemical effects in living organisms. In this review, the works published in last 20 years are covered, and the main topics related to sample preparation methods and analytical techniques commonly used for fluorine, chlorine, bromine, and iodine determination in biological samples, food, drugs, and plants used as food or with medical applications are discussed. The commonest sample preparation methods, as extraction and decomposition using combustion and pyrohydrolysis, are reviewed, as well as spectrometric and electroanalytical techniques, spectrophotometry, total reflection X-ray fluorescence, neutron activation analysis, and separation systems using chromatography and electrophoresis. On this aspect, the main analytical challenges and drawbacks are highlighted. A discussion related to the availability of certified reference materials for evaluation of accuracy is also included, as well as a discussion of the official methods used as references for the determination of halogens in the samples covered in this review.

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Laser ablation ICP-MS for quantitative biomedical applications

Cited by 114 publications

References 98 publications

Quantitative imaging of 2 nm monolayer-protected gold nanoparticle distributions in tissues using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS)

Quantitative imaging of 2 nm monolayer-protected gold nanoparticle distributions in tissues using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS)

LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

Analytical methods for the determination of halogens in bioanalytical sciences: a review

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