Improvements in Gene Editing Technology Boost Its Applications in Livestock

Périssé, Iuri Viótti; Fan, Zhiqiang; Сингина, Г. Н.; White, Kenneth L.; Polejaeva, Irina A.

doi:10.3389/fgene.2020.614688

Cited by 46 publications

(47 citation statements)

References 273 publications

(290 reference statements)

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“…Since the birth of Dolly the sheep derived from an adult mammary gland cell, SCNT has been heralded as a promising assisted reproductive technology with a broad range of applications in regenerative medicine, livestock industries, and pharmaceutical manufacturing [ 1–5 ]. Recent advancements in gene-editing technologies, especially the CRISPR/Cas9 system, have further enhanced the use of SCNT in livestock animals including pigs, goats, cattle, and sheep for the production of useful phenotypes such as disease resistance and high meat/milk production [ 6 ]. Meanwhile, there have been many attempts to increase the efficiency of SCNT, especially in mice.…”

Section: Introductionmentioning

confidence: 99%

Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†

Kamimura

Inoue

Mizutani

et al. 2021

Biology of Reproduction

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In mammalian cloning by somatic cell nuclear transfer (SCNT), treatment of reconstructed embryos with histone deacetylase (HDAC) inhibitors improves efficiency. So far, most of those used for SCNT are hydroxamic acid derivatives—such as trichostatin A—characterized by their broad inhibitory spectrum. Here, we examined whether mouse SCNT efficiency could be improved using chlamydocin analogues, a family of newly designed agents that specifically inhibit Class I and IIa HDACs. Development of SCNT-derived embryos in vitro and in vivo revealed that four out of five chlamydocin analogues tested could promote the development of cloned embryos. The highest pup rates (7.1 to 7.2%) were obtained with Ky-9, similar to those achieved with trichostatin A (7.2 to 7.3%). Thus, inhibition of Class I and/or IIa HDACs in SCNT-derived embryos is enough for significant improvements in full-term development. In mouse SCNT, the exposure of reconstructed oocytes to HDAC inhibitors is limited to 8–10 h because longer inhibition with Class I inhibitors causes a 2-cell developmental block. Therefore, we used Ky-29, with higher selectivity for Class IIa than Class I HDACs for longer treatment of SCNT-derived embryos. As expected, 24-h treatment with Ky-29 up to the 2-cell stage did not induce a developmental block, but the pup rate was not improved. This suggests that the 1-cell stage is a critical period for improving SCNT cloning using HDAC inhibitors. Thus, chlamydocin analogues appear promising for understanding and improving the epigenetic status of mammalian SCNT-derived embryos through their specific inhibitory effects on HDACs.

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Section: Introductionmentioning

confidence: 99%

Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†

Kamimura

Inoue

Mizutani

et al. 2021

Biology of Reproduction

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“…By designing and synthesizing a short nucleic acid base on the sequence near PAM of the target gene and attaching it to the corresponding vector (such as PX330, PX459) by enzyme digestion, researchers can use the CRISPR/Cas9 system to modify specific genes. This technique has been widely studied and applied to improve livestock heredity, reproduction, and nutrition levels [ 49 ]. CRISPR/Cas9 was used to improve the meat production trait of various livestock by efficiently knocking out MSTN [ 50 , 51 , 52 ].…”

Section: Gene Editing Tools and Their Application In Farm Animalsmentioning

confidence: 99%

Application of Gene Editing Technology in Resistance Breeding of Livestock

Wang

Huang

et al. 2022

Life

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As a new genetic engineering technology, gene editing can precisely modify the specific gene sequence of the organism’s genome. In the last 10 years, with the rapid development of gene editing technology, zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), and CRISPR/Cas9 systems have been applied to modify endogenous genes in organisms accurately. Now, gene editing technology has been used in mice, zebrafish, pigs, cattle, goats, sheep, rabbits, monkeys, and other species. Breeding for disease-resistance in agricultural animals tends to be a difficult task for traditional breeding, but gene editing technology has made this easier. In this work, we overview the development and application of gene editing technology in the resistance breeding of livestock. Also, we further discuss the prospects and outlooks of gene editing technology in disease-resistance breeding.

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“…Genetically modified embryos may be produced by SCNT, where the modifications have been induced in the primary cells that serve as donors of nuclei, or directly in zygotes using zygote injection (ZI) or electroporation. When using SCNT, all embryos have a uniform genotype, show no mosaicism, and donor cells can be screened thoroughly for possible off-target effects, making this the preferred technique for producing genome edited animals (reviewed in [ 109 ]). Nevertheless, cloning artefacts (see Section 2.6 ) that possibly alter developmental mechanisms must be considered and closely monitored by implementing appropriate controls [ 110 ].…”

Section: Genetic Manipulationsmentioning

confidence: 99%

A New Toolbox in Experimental Embryology—Alternative Model Organisms for Studying Preimplantation Development

Springer

Wolf

Simmet

2021

JDB

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Preimplantation development is well conserved across mammalian species, but major differences in developmental kinetics, regulation of early lineage differentiation and implantation require studies in different model organisms, especially to better understand human development. Large domestic species, such as cattle and pig, resemble human development in many different aspects, i.e., the timing of zygotic genome activation, mechanisms of early lineage differentiations and the period until blastocyst formation. In this article, we give an overview of different assisted reproductive technologies, which are well established in cattle and pig and make them easily accessible to study early embryonic development. We outline the available technologies to create genetically modified models and to modulate lineage differentiation as well as recent methodological developments in genome sequencing and imaging, which form an immense toolbox for research. Finally, we compare the most recent findings in regulation of the first lineage differentiations across species and show how alternative models enhance our understanding of preimplantation development.

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Improvements in Gene Editing Technology Boost Its Applications in Livestock

Cited by 46 publications

References 273 publications

Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†

Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†

Application of Gene Editing Technology in Resistance Breeding of Livestock

A New Toolbox in Experimental Embryology—Alternative Model Organisms for Studying Preimplantation Development

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