Improvement scheme for the determination of arsenic species in mussel and fish tissues

Lagarde, Florence; Amran, M. B.; Leroy, M. J. F.; Demesmay, Claire; Ollé, M.; Lamotte, Adrien Frizon de; Muntau, H.; Michel, Pierre; Thomas, Patrick R.; Caroli, Sergio; Larsen, Erik Huusfeldt; Bonner, P.; Rauret, G.; Foulkes, Mike E.; Howard, A.G.; Griepink, B.; Maier, E. A.

doi:10.1007/s002160051129

Cited by 12 publications

(5 citation statements)

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“…Although it might be thought that inorganic arsenic was not detected in the samples analyzed, authors such as Brooke and Evans 9 have determined that inorganic arsenic represents between 1-5% of total arsenic in seafood products. We consider that the absence of data concerning the levels of inorganic arsenic in the work reported by Lagarde et al 19 is due to deficient extraction of the inorganic arsenic, since the same authors 17 indicate that the wide variability in the extraction of arsenic species (50-80% of total As) reported by the various laboratories is the result of incomplete extraction of the compounds studied or the existence of forms of arsenic which were not identified or could not be extracted with watermethanol.…”

Section: Extraction With Methanolmentioning

confidence: 92%

“…Interlaboratory studies carried out by Lagarde et al, 19 based on extraction with water-methanol (1 : 1, v/v) of the arsenic species present in real samples of mussel tissue, reveal that the inorganic form, specifically As(iii), was not identified by any of those taking part in the study, and only a few of them reported traces of As(v). Although it might be thought that inorganic arsenic was not detected in the samples analyzed, authors such as Brooke and Evans 9 have determined that inorganic arsenic represents between 1-5% of total arsenic in seafood products.…”

Section: Extraction With Methanolmentioning

confidence: 95%

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Optimization of the solubilization, extraction and determination of inorganic arsenic [As(III) + As(V)] in seafood products by acid digestion, solvent extraction and hydride generation atomic absorption spectrometry

Muñoz¹,

Vélez²,

Montoro³

1999

Analyst

138

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A method for the selective quantitative determination of inorganic arsenic [As(III) + As(V)] in seafood was developed. In order to do so, various procedures for the solubilization and extraction of inorganic arsenic quoted in the literature were tested. None provided satisfactory recoveries for As(III) and As(V) in real samples. Consequently, a methodology was developed which included solubilization with HCl and subsequent extraction with chloroform. The arsenic was solubilized in 9 mol l-1 hydrochloric acid. After reduction by hydrobromic acid and hydrazine sulfate, the inorganic arsenic was extracted into chloroform, back-extracted into 1 mol l-1 HCl, dry-ashed, and quantified by hydride generation-atomic absorption spectrometry (HG-AAS). The analytical features of the method are as follows: detection limit, 3.07 ng g-1 As (fresh mass); precision (RSD), 4.0%; recovery, As(III) 99%, As(V) 96%. In the optimized conditions, other arsenic species--dimethylarsinic acid (DMA), arsenobetaine (AB), arsenocholine (AC) and tetramethylarsonium-ion (TMA+)--were not co-extracted. However, different percentages of minor species were extracted with chloroform: monomethylarsonic acid (MMA) 100%, and trimethylarsine oxide (TMAO) 3-10%. Real samples and reference materials of seafood (DORM-1, DORM-2, TORT-2, CRM-278 and SRM-1566a) were analyzed. The analysis of DORM-1 provided an inorganic arsenic value of 124 +/- 4 ng g-1 As, dry mass (dm), which is very close to the value obtained by other authors using high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) and ionic chromatography-hydride generation-atomic absorption spectrometry (IC-HG-AAS).

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Section: Extraction With Methanolmentioning

confidence: 92%

Section: Extraction With Methanolmentioning

confidence: 95%

Optimization of the solubilization, extraction and determination of inorganic arsenic [As(III) + As(V)] in seafood products by acid digestion, solvent extraction and hydride generation atomic absorption spectrometry

Muñoz¹,

Vélez²,

Montoro³

1999

Analyst

138

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“…Arsenic species have been characterized in some CRMs but only a few materials dedicated to speciation are available. 15,16 The objectives of this research were (i) to investigate in detail speciation of arsenic in an oyster test reference material by multi-dimensional (employing consecutive orthogonal separation mechanisms) HPLC-ICP-MS and ES-MS-MS and (ii) to optimize the conditions of single mechanism HPLC-ICP-MS leading to a chromatographically pure arsenobetaine peak and thus allowing the quantification of this species.…”

Section: Introductionmentioning

confidence: 99%

Investigation of arsenic speciation in oyster test reference material by multidimensional HPLC-ICP-MS and electrospray tandem mass spectrometry (ES-MS-MS)

McSheehy

Pohl

Łobiński

et al. 2001

Analyst

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Multidimensional (size-exclusion-anion-exchange-cation-exchange) liquid chromatography with ICP-MS detection was developed to produce a map of water-soluble species in an oyster test reference material. The presence of arsenobetaine, trimethyl(2-carboxyethyl)arsonium inner salt, arsenocholine, dimethylarsonic acid, tetramethylammonium ion, As(v) and two arsenosugars was demonstrated by ES-MS-MS. A previously unreported compound was isolated and identified by ES-MS-MS as 5-dimethylarsinoyl-beta-ribofuranose. Anion-exchange chromatography was optimized to produce a chromatographically pure peak of arsenobetaine (accounting for ca. 64% of all water-soluble As present) that was used to quantify this compound.

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“…Microwave oven combustion was performed for 22 min in Teflon chambers MLS 1200 MEGA "exhaust module" EM-45/A (Milestone, Italy) (Legarde et al 1999). Digested tissue was diluted with double distilled water up to 100 ml.…”

Section: Preparation Of Tissue and Arsenic Measurementmentioning

confidence: 99%

Seasonal variations of arsenic in mussels Mytilus galloprovincialis

2004

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Total arsenic concentration in the edible part of mussels Mytilus galloprovincialis was evaluated seasonally in the coastal area of Rijeka Bay (North Adriatic Sea, Croatia). Sampling stations were located close to the City of Bakar with no industrial facilities (site 1), in the vicinity of the oil refinery and oil thermoelectric power plant (Urinj, site 2), and 4 miles away from the Plomin coal thermoelectric power plant (Brseč village, site 3). Additionally, the concentration of arsenic in the tail muscle of the lobster Nephrops norvegicus, collected in Rijeka Bay, was studied. During winter at sites 2 and 3, the total arsenic in the edible part of the mussels was 16.4 mg As/kg FW (FW=fresh weight) and 4.38 mg As/ kg FW, respectively, and increased during springtime at site 2 (6.5 mg As/kg FW) compared to the rest of the year, when individual total arsenic concentration at all sites ranged from 1.7 to 3.7 mg As/kg FW. In the winter (sites 2 and 3) and springtime (site 2) there was no correlation between the length of the mussel shell and the arsenic concentration in the edible part of the mussels. In the other seasons, at sites 1, 2 and 3, there was a correlation between arsenic in the edible part of mussels and shell length in most cases (correlation coefficients r varied from 0.64 to 0.85; P <0.05 to P <0.01). Correlation between shell length (in the narrow range of shell lengths from 3.4 to 5.0 cm) and arsenic in the edible part of the mussels shows linearity with a high regression coefficient ( r =0.914; P <0.001). The increase of arsenic in the mussels during winter and spring was suggested at least partially as a result of a low nutritional status, i.e. reduced weight of the mussels' edible part during winter. In addition, a linear relationship was found between body length and arsenic concentration in the tail muscle (mean 17.11€4.48 mg As/kg FW) of the Norway lobster.

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Improvement scheme for the determination of arsenic species in mussel and fish tissues

Cited by 12 publications

References 0 publications

Optimization of the solubilization, extraction and determination of inorganic arsenic [As(III) + As(V)] in seafood products by acid digestion, solvent extraction and hydride generation atomic absorption spectrometry

Optimization of the solubilization, extraction and determination of inorganic arsenic [As(III) + As(V)] in seafood products by acid digestion, solvent extraction and hydride generation atomic absorption spectrometry

Investigation of arsenic speciation in oyster test reference material by multidimensional HPLC-ICP-MS and electrospray tandem mass spectrometry (ES-MS-MS)

Seasonal variations of arsenic in mussels Mytilus galloprovincialis

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