Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

Abstract

Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L total sugar) pretreated with activated charcoal and yeast extract (50 g.L). The HA produced exhibited a high molecular weight of 1.35 × 10 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid.

Study on TEMPO-Mediated Oxidation of N-Succinyl Chitosan and the Water Retention Property

“…In the past two decades, hyaluronic acid (HA), composed of d -glucuronic acid and N -acetyl- d -glucosamine, has played a critical role in cosmetics and clinical medicine because of its unique physical and chemical properties [ 1 ]. Despite the significant progress that has been achieved on the microbial production of HA, the continuous rise in the cost of raw materials weakens the commercial competitiveness of microbial HA production [ 2 , 3 , 4 , 5 ].…”

Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract

“…The production of bacterial hyaluronic acid is now ensured by Streptococcus zooepidemicus, in order to avoid its extraction from mammalian tissues. Different culture conditions were described to improve its production for future applications for cosmetic and biomedical applications [23,24].…”

Films Based on Blends of Polyvinyl Alcohol and Microbial Hyaluronic Acid

“…More recently, the polymer has been produced by the fermentation of Streptococci, thereby eliminating the possibility of inter-species disease transfer [6,7]. In addition, the use of low-cost renewable resources and agro-industrial by-products as fermentation media contributes to making microbial HA production economically feasible [8][9][10].…”

“…The HA production and purification were conducted based on the method described by Pan and coauthors [9]. Batch fermentation in a bioreactor (Tecnal, Piracicaba, SP, Brazil) was conducted in a fermentation medium containing clarified sugarcane molasses with 85.35 g L −1 total sugar content, 50 g L -1 yeast extract, 2.5 g L -1 K2HPO4, 2.0 g L -1 NaCl and 1.5 g L -1 MgSO4 at pH 8.0 for 24 h. Cell-free supernatant was precipitated with ethanol at a 1.5:1.0 (v/v) ratio of ethanol:supernatant at 4 °C for 1 h. The HA was redissolved in a 0.15 mol L −1 NaCl solution.…”