Improvement on the Performance of Bone Regeneration of Calcium Sulfate Hemihydrate by Adding Mineralized Collagen

Liu, Huanye; Liu, Xi; Zhang, Liping; Ai, Hongjun; Cui, Fuzhai

doi:10.1089/ten.tea.2009.0669

Cited by 19 publications

(27 citation statements)

References 35 publications

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“…34 In agreement with this study, we also found that the n-CDHA/MAC/CSH implants accelerated new bone formation in the treatment of critical size defects, compared to the pure CSH implants. Moreover, the n-CDHA/MAC/CSH implant contacted with the host bone intimately, without causing any evident adverse effect on surrounding soft tissues.…”

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confidence: 91%

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Qi¹,

Hong²,

Qiao³

et al. 2013

IJN

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A novel injectable bone cement was developed by integration of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer (n-CDHA/MAC) and calcium sulfate hemihydrate (CSH; CaSO 4 · 1/2H 2 O). The structure, setting time, and compressive strength of the cement were investigated. The results showed that the cement with a liquid to powder ratio of 0.8 mL/g exhibited good injectability and appropriate setting time and mechanical properties. In vitro cell studies indicated that MC3T3-E1 cells cultured on the n-CDHA/MAC/CSH composite spread well and showed a good proliferation state. The alkaline phosphatase activity of the MC3T3-E1 cells cultured on the n-CDHA/MAC/CSH composite was significantly higher than that of the cells on pure CSH at 4 and 7 days of culture. The n-CDHA/MAC/CSH cement was implanted into critical size defects of the femoral condyle in rabbits to evaluate its biocompatibility and osteogenesis in vivo. Radiological and histological results indicated that introduction of the n-CDHA/MAC into CSH enhanced new bone formation, and the n-CDHA/MAC/CSH cement exhibited good biocompatibility and degradability. In conclusion, the injectable n-CDHA/MAC/CSH composite cement has a significant clinical advantage over pure CSH cement, and may be a promising bone graft substitute for the treatment of bone defects.

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supporting

confidence: 91%

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Qi¹,

Hong²,

Qiao³

et al. 2013

IJN

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“…To eliminate the osteogenic differentiation effect of Dex, we cultured BMSCs in the absence of Dex and evaluated the osteogenesis of BMSCs. The biological behavior of BMSCs has been previously described [58]. …”

Section: Discussionmentioning

confidence: 99%

Activation of the ERK1/2 Signaling Pathway during the Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Substrates Modified with Various Chemical Groups

Bai

et al. 2013

BioMed Research International

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The current study examined the influence of culture substrates modified with the functional groups –OH, –COOH, –NH2, and –CH3 using SAMs technology, in conjunction with TAAB control, on the osteogenic differentiation of rabbit BMSCs. The CCK-8 assay revealed that BMSCs exhibited substrate-dependent cell viability. The cells plated on –NH2- and –OH-modified substrates were well spread and homogeneous, but those on the –COOH- and –CH3-modified substrates showed more rounded phenotype. The mRNA expression of BMSCs revealed that –NH2-modified substrate promoted the mRNA expression and osteogenic differentiation of the BMSCs. The contribution of ERK1/2 signaling pathway to the osteogenic differentiation of BMSCs cultured on the –NH2-modified substrate was investigated in vitro. The –NH2-modified substrate promoted the expression of integrins; the activation of FAK and ERK1/2. Inhibition of ERK1/2 activation by PD98059, a specific inhibitor of the ERK signaling pathway, blocked ERK1/2 activation in a dose-dependent manner, as revealed for expression of Cbfα-1 and ALP. Blockade of ERK1/2 phosphorylation in BMSCs by PD98059 suppressed osteogenic differentiation on chemical surfaces. These findings indicate a potential role for ERK in the osteogenic differentiation of BMSCs on surfaces modified by specific chemical functional groups, indicating that the microenvironment affects the differentiation of BMSCs. This observation has important implications for bone tissue engineering.

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“…Calcium sulfate (CaSO 4 ) has been used extensively as bone defects fillers on account of the superb biocompatibility and osteoconductive, which can degrade and be resorbed completely by surrounding bone tissue [9,10,11,12]. However, CaSO 4 fails to provide effective support for the defect site in the process of bone repair, due to its fast disintegration and poor mechanical strength [13].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone Repair

Shuai

Zhou

et al. 2015

Materials

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Calcium sulfate (CaSO4), as a promising tissue repair material, has been applied widely due to its outstanding bioabsorbability and osteoconduction. However, fast disintegration, insufficient mechanical strength and poor bioactivity have limited its further application. In the study, CaSO4 scaffolds fabricated by using selective laser sintering were improved by adding 45S5 bioglass. The 45S5 bioglass enhanced stability significantly due to the bond effect of glassy phase between the CaSO4 grains. After immersing for four days in simulated body fluid (SBF), the specimens with 45S5 bioglass could still retain its original shape compared as opposed to specimens without 45S5 bioglass who experienced disintegration. Meanwhile, its compressive strength and fracture toughness increased by 80% and 37%, respectively. Furthermore, the apatite layer was formed on the CaSO4 scaffolds with 45S5 bioglass in SBF, indicating good bioactivity of the scaffolds. In addition, the scaffolds showed good ability to support the osteoblast-like cell adhesion and proliferation.

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Improvement on the Performance of Bone Regeneration of Calcium Sulfate Hemihydrate by Adding Mineralized Collagen

Cited by 19 publications

References 35 publications

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Activation of the ERK1/2 Signaling Pathway during the Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Substrates Modified with Various Chemical Groups

Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone Repair

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