Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Qi, Xiaotong; Hong, Liu; Qiao, Bo; Li, Weichao; Xinyan, Hao; Wu, Jun; Su, Bao; Jiang, Dianming

doi:10.2147/ijn.s54289

Cited by 17 publications

(12 citation statements)

References 38 publications

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“…The rabbits ( n = 6) were euthanized to harvest specimens at 4 and 12 weeks after operation. The samples were then fixed for 2 days in 4% paraformaldehyde and scanned with micro-CT (Scanco, Brüttisellen, Switzerland) following standard protocol (70 kV, 114 A, and 20 m) [ 51 ]. Initial binary images were reconstructed to obtain 3D images using the built-in software.…”

Section: Methodsmentioning

confidence: 99%

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Yan

Liu

et al. 2021

Stem Cell Res Ther

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Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a promising breakthrough. Methods In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Effect of EMF on the proliferation and chondrogenic differentiation potential was evaluated in vitro. 4 mm femoral condyle defect was constructed in rabbits. The scaffolds loaded with BMSCs were implanted into the defects with or without EMF treatment. Effects of the combination treatment of the EMF and composite scaffold on rabbit osteochondral defect was detected in vivo. Results In vitro experiments showed that EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin signaling pathway. In vivo results further confirmed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. Conclusion Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.

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Section: Methodsmentioning

confidence: 99%

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Yan

Liu

et al. 2021

Stem Cell Res Ther

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“…041-0224. 59 Moreover, the peak formed at 2θ∼11°indicates the presence of the CDHA phase in the composite. 38 The FESEM image in Figure 7(b) shows a broad particle size distribution in the composite.…”

Section: Characterization Of the Composite Pastesmentioning

confidence: 99%

Preparation and characterization of injectable gelatin/alginate/chondroitin sulfate/α-calcium sulfate hemihydrate composite paste for bone repair application

2022

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In this study, a group of injectable composite pastes with a novel formulation consisting of two inorganic components: α-calcium sulfate hemihydrate (α-CSH, P/L = 1.8–2.1 g/ml) and calcium-deficient hydroxyapatite (CDHA, P/L = 0.1 g/ml) nanoparticles; and three biopolymers: gelatin (2, 4 wt. %), alginate (1, 1.5 wt. %), and chondroitin sulfate (0.5 wt. %) were carefully prepared and thoroughly characterized with commensurate characterizations. The composite sample composed of gelatin (2 wt. %), alginate (1.5 wt. %), chondroitin sulfate (0.5 wt. %), and also CDHA nanoparticles and α-CSH with P/L ratios of 0.1 and 2.1 g/ml, respectively, exhibited optimal properties in terms of injectability, anti-washout performance, and rheological characteristics. After 14 days of immersion of the chosen sample in the simulated body fluid medium, a dense layer of apatite was formed on the surface of the composite paste. The cellular in vitro tests, such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT), alkaline phosphatase assay, 4ʹ,6-diamidino-2-phenylindole staining, and cellular attachment, revealed the desirable response of MG-63 cells to the composite paste. The chondroitin sulfate significantly improved the injectability, anti-washout performance, and cellular response of the samples. Considering the promising features of the composite paste prepared in this research work, it could be considered as an alternative injectable bioactive material for bone repair applications. [Formula: see text]

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“…The ambiguity of the term reflects the ongoing development of insights into how biomaterials interact with the humanbody and eventually how those interactions determine the clinical success of such material [44]. Biocompatibility is essentialfor implantable materials and is often the result of complex interactions at the host-material interface.A good biocompatible material should not elicit a host response after implantation [45].…”

Section: Biocompatibility and Degradibilitymentioning

confidence: 99%

Calcium Sulfate as a Scaffold for Bone Tissue Engineering: A Descriptive Review

Fernandes¹,

Abhyankar²,

O’Dell³

2021

JDODT

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In regenerative approaches, scaffolds play a crucial role in defining the 3-D anatomical shape and microenvironment for regenerative cells, while maintaining space, favoring regeneration of new bone, and preventing soft tissue migration in the bony lesion. A variety of materials have been explored as scaffolds for bone tissue regeneration. Metals are generally inappropriate for such applications because they lack the desired degradability in a biological environment requiring a second surgery. Synthetic polymers can provide physical strength and engineered chemical properties however, they do not have optimal ease of handling or predictable biodegradation rate. In contrast to these scaffolds, calcium sulfate, also known as Plaster of Paris, is a highly biocompatible and biodegradable material that is one of the simplest synthetic bone-like grafts. It has been sued as a graft material in orthopedics and dentistry for more than one hundred years. The present review illustrates and examines current advances regarding calcium sulfate sources, various properties,scaffold forms, and clinical applications for bone tissue engineering.Additionally,it also describesNanocrystallinecalcium sulfate. Keywords: Calcium sulfate; Gypsum; Plaster of Paris; Graft; Regeneration

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Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Cited by 17 publications

References 38 publications

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Preparation and characterization of injectable gelatin/alginate/chondroitin sulfate/α-calcium sulfate hemihydrate composite paste for bone repair application

Calcium Sulfate as a Scaffold for Bone Tissue Engineering: A Descriptive Review

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