Improvement of Vicia-micronucleus test for assessment of soil quality: A proposal for international standardization

Foltête, Anne-Sophie; Dhyèvre, Adrien; Férard, Jean-François; Cotelle, Sylvie

doi:10.1016/j.chemosphere.2011.08.026

Cited by 17 publications

(9 citation statements)

References 34 publications

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“…The mutagenic effect of MH has been well known for a longtime [34]. Although there are no published data available on the chemically induced clastogenic effect for Brachypodium, the mutagenic effect of MH has previously been demonstrated for a wide of range other plant species such as Crepis capillaris [3], Allium cepa [28, 35, 36], Vicia faba [37] and Tradescantia [38]. In contrast, according to Gichner et al [39], MH is not mutagenic in Arabidopsis cells.…”

Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

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Brachypodium distachyon (Brachypodium) is now intensively utilized as a model grass species in various biological studies. Its favorable cytological features create a unique foundation for a convenient system in mutagenesis, thereby potentially enabling the ‘hot spots’ and ‘cold spots’ of DNA damage in its genome to be analyzed. The aim of this study was to analyze the involvement of 5S rDNA, 25S rDNA, the Arabidopsis-type ()n telomeric sequence and the Brachypodium-originated centromeric BAC clone CB33J12 in the micronuclei formation in Brachypodium root tip cells that were subjected to the chemical clastogenic agent maleic hydrazide (MH). To the best of our knowledge, this is the first use of a multicolor fluorescence in situ hybridization (mFISH) with four different DNA probes being used simultaneously to study plant mutagenesis. A quantitative analysis allowed ten types of micronuclei, which were characterized by the presence or absence of specific FISH signal(s), to be distinguished, thus enabling some specific rules governing the composition of the MH-induced micronuclei with the majority of them originating from the terminal regions of chromosomes, to be identified. The application of rDNA sequences as probes showed that 5S rDNA-bearing chromosomes are involved in micronuclei formation more frequently than the 25S rDNA-bearing chromosomes. These findings demonstrate the promising potential of Brachypodium to be a useful model organism to analyze the effects of various genotoxic agents on the plant nuclear genome stability, especially when the complex FISH-based and chromosome-specific approaches such as chromosome barcoding and chromosome painting will be applied in future studies.

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Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

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“…The overexpression of phytotoxicity by the use of soil extracts might be a result of improper representation of soil solution under equilibrium (Matschonat and Vogt, 1997). As suggested by Foltête et al (2011), the main advantage of direct contact is that interactions occur between the soil and the test organisms, so that mobility and bioavailability of the contaminants are represented by the test.…”

Section: Discussionmentioning

confidence: 99%

“…The same approach was used in previous studies to assess the phytotoxicity in soil extracts after OMW application (Mekki et al, 2007;Saadi et al, 2007). However, it is questionable whether soil extract-based bioassays reflect the phytotoxicity of the soil in situ (Leitgib et al, 2007;Foltête et al, 2011); artifacts that may occur could be related to inefficient or selectively efficient extraction of toxic or stimulatory compounds, with consequent changes to the net synergistic effect of the soil solution.…”

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confidence: 99%

Fate of Soil-Applied Olive Mill Wastewater and Potential Phytotoxicity Assessed by Two Bioassay Methods

et al. 2013

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Controlled land spreading of untreated olive mill wastewater (OMW) has been widely practiced as a means of its disposal. However, potential phytotoxic effects are critical for the selection of sites and crop types and for proper synchronization between land application and cropping. This study traced the fate of dissolved organic carbon (DOC), total phenols (TP), electrical conductivity, pH, microbial counts, and phytotoxicity to cress ( L.) after soil application at doses equivalent to 80, 160, and 320 m ha. Vertisol (fine-clayey) and Loess (sandy loam) soils were treated and incubated at 12 or 25°C and at moisture contents maintained at 70% of field water capacity or gradually reduced from 70 to 20% without compensation. Temperature, rather than moisture content, had a major effect on removal rates of DOC and TP. The maximum combined effect of warm temperature and higher moisture content resulted in removal rates greater than those under cooler, drier conditions by factors of up to 1.8 and 4.1 for DOC and TP, respectively. Favorable biodegradation conditions were indicated by increased numbers of total soil microorganisms and fungi by factors of up to 26 and 5, respectively. A whole-soil bioassay was developed to assess the dynamics of residual soil phytotoxicity after OMW application. Phytotoxicity measurement in soil extract generally showed stronger inhibition or stimulation activity than measurement in whole soil, depending on soil type and OMW dose. The newly developed bioassay seems to be useful for the refinement of general recommendations regarding permitted OMW application doses.

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“…Le potentiel génotoxique des sols a été évalué à travers leurs lixiviats en se servant du test de micronoyaux appliqué au niveau des extrémités racinaires de Vicia faba. Ce test est considéré parmi les meilleurs outils disponibles pour étudier la biodisponibilité des polluants du sol et d'eau, leurs interactions possibles et les effets potentiels de la pollution non caractérisée (FOLTÊTE et al, 2011;COTELLE et al, 2015).…”

Section: Introductionunclassified

Évaluation de la qualité des eaux de la nappe de Téboulba : risque de contamination des sols agricoles

Souguir

Hörmann

Hachicha

2019

rseau

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Improvement of Vicia-micronucleus test for assessment of soil quality: A proposal for international standardization

Cited by 17 publications

References 34 publications

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Fate of Soil-Applied Olive Mill Wastewater and Potential Phytotoxicity Assessed by Two Bioassay Methods

Évaluation de la qualité des eaux de la nappe de Téboulba : risque de contamination des sols agricoles

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