Improvement of the Vomeronasal Organ Ablation in the Rat

Igarashi, Noriyuki; Tauchi, Kiyonori; Imamichi, Tomonori; Saitô, Tôru

doi:10.1538/expanim1978.37.3_355

Cited by 2 publications

(2 citation statements)

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“…Different strategies have been used to achieve ablation in vivo. Surgical ablation can be used to remove a specific organ or an embryonic precursor during development (Davidson et al, 1999;Igarashi et al, 1988). Laser surgery can be used to ablate single cells in organisms having welldefined and a limited number of cell types such as Caenorhabditis elegans (Kimble, 1981).…”

Section: Introductionmentioning

confidence: 99%

TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis

Pasquier¹,

Chesneau²,

Ymlahi-Ouazzani³

et al. 2007

Genesis

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Xenopus is a well proven model for a wide variety of developmental studies, including cell lineage. Cell lineage in Xenopus has largely been addressed by injection of tracer molecules or by micro-dissection elimination of blastomeres. Here we describe a genetic method for cell ablation based on the use of tBid, a direct activator of the mitochondrial apoptotic pathway. In mammalian cells, cross-talk between the main apoptotic pathways (the mitochondrial and the death domain protein pathways) involve the pro-death protein BID, the active form of which, tBID, results from protease truncation and translocation to mitochondria. In transgenic Xenopus, restricting tBID expression to the lens-forming cells enables the specific ablation of the lens without affecting the development of other eye structures. Thus, overexpression of tBid can be used in vivo as a tool to eliminate a defined cell population by apoptosis in a developing organism and to evaluate the degree of autonomy or the inductive effects of a specific tissue during embryonic development.

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Section: Introductionmentioning

confidence: 99%

TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis

Pasquier¹,

Chesneau²,

Ymlahi-Ouazzani³

et al. 2007

Genesis

View full text Add to dashboard Cite

show abstract

“…Different strategies have been used to achieve ablation in vivo. Surgical ablation can be used to remove a specific organ or an embryonic precursor during development (4,5). Laser surgery can be used to ablate single cells in organisms with well-defined cell lineages and with a limited number of cells such as Caenorhabditis elegans (6).…”

Section: Introductionmentioning

confidence: 99%

Bigenic Cre/loxP, pu tk conditional genetic ablation

Chen

Levasseur²,

Vaishnav³

et al. 2004

Nucleic Acids Research

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Genetic ablation experiments are used to resolve problems regarding cell lineages and the in vivo function of certain groups of cells. We describe a two-component conditional ablation technology using a mouse carrying an X-linked puDeltatk transgene, which is only activated in cells expressing Cre. Ablation of the Cre-expressing cells can be temporally regulated by the time of ganciclovir (GCV) administration. This strategy was demonstrated using a Col2Cre transgenic line. Differentiating chondrocytes in bigenic animals could be ablated at different developmental stages resulting in disorganized growth plates and dwarfism. Macrocephaly, macroglossia and umbilical hernia were also observed in ablated 18.5 dpc embryos. Crosses between the puDeltatk selector transgenic line and existing cre lines will facilitate numerous temporally regulated tissue-specific ablation experiments.

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Improvement of the Vomeronasal Organ Ablation in the Rat

Cited by 2 publications

References 0 publications

TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis

TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis

Bigenic Cre/loxP, pu tk conditional genetic ablation

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