TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in <i>Xenopus laevis</i>

Pasquier, David Du; Chesneau, Albert; Ymlahi-Ouazzani, Qods; Boistel, Renaud; Pollet, Nicolas; Ballagny, Chantal; Sachs, Laurent M.; Demeneix, Barbara; Mazabraud, A

doi:10.1002/dvg.20252

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…(c) X-ray synchrotron phase-contrast microtomography We used the ID19 long (150 m) imaging beamline of the European Synchrotron Radiation Facility (Grenoble, France) with large spatial coherence (Du Pasquier et al 2007;Guigay et al 2007; Van der Meijden et al 2007). We used a monochromatic beam with a bandwidth of DE/E of 10 24 obtained with a double Si111 Bragg monochromator.…”

Section: Methodsmentioning

confidence: 99%

Assisted walking in Malagasy dwarf chamaeleons

et al. 2010

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Chamaeleons are well known for their unique suite of morphological adaptations. Whereas most chamaeleons are arboreal and have long tails, which are used during arboreal acrobatic manoeuvres, Malagasy dwarf chamaeleons (Brookesia) are small terrestrial lizards with relatively short tails. Like other chamaeleons, Brookesia have grasping feet and use these to hold on to narrow substrates. However, in contrast to other chamaeleons, Brookesia place the tail on the substrate when walking on broad substrates, thus improving stability. Using threedimensional synchrotron X-ray phase-contrast imaging, we demonstrate a set of unique specializations in the tail associated with the use of the tail during locomotion. Additionally, our imaging demonstrates specializations of the inner ear that may allow these animals to detect small accelerations typical of their slow, terrestrial mode of locomotion. These data suggest that the evolution of a terrestrial lifestyle in Brookesia has gone hand-in-hand with the evolution of a unique mode of locomotion and a suite of morphological adaptations allowing for stable locomotion on a wide array of substrates.

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Section: Methodsmentioning

confidence: 99%

Assisted walking in Malagasy dwarf chamaeleons

et al. 2010

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“…Proteins-Five members of the Xenopus Bcl-2 family of proteins (xBcl-w/xR1, xBcl-x L /xR11, xBax, xBid, and xBok) were functionally characterized in the previous studies (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). In addition, partial or complete nucleotide sequences of 10 Bcl-2 family proteins (xBcl-2, xBcl-B, xBcl-G, xBcl2L12, xBcl2L13, xMcl-1, xBak, xBim, xBmf, and xNoxa) were deposited in the Xenopus nucleotide database, but the functions of their protein products remained uncharacterized (42)(43)(44).…”

Section: Identification and Rt-pcr Analysis Of Xenopus Bcl-2 Familymentioning

confidence: 99%

“…They are classified as either anti-apoptotic or pro-apoptotic, and the functional balance between these two groups controls the mitochondria-mediated intrinsic pathway of apoptosis. Although more than 10 members of the Bcl-2 family of proteins have been identified in Xenopus laevis (simply referred to as Xenopus hereafter), their functional characterizations are not yet complete (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

mentioning

confidence: 99%

Anti-apoptotic Activity and Proteasome-mediated Degradation of Xenopus Mcl-1 Protein in Egg Extracts

Tsuchiya

Yamashita

2011

Journal of Biological Chemistry

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Xenopus egg extracts execute spontaneous apoptosis without the requirement of transcription and translation, and this intrinsic mechanism is supposed to be involved in the physiological elimination of aged eggs. Although apoptosis in this system is carried out by maternally stockpiled materials, the endogenous apoptosis regulators present in egg extracts are still poorly characterized. Here we examined the mRNA expression profiles and apoptosis-regulating functions of 13 Xenopus Bcl-2 family proteins in egg extracts. Among these, we found that endogenous Xenopus Mcl-1 (xMcl-1) physiologically inhibited apoptosis by counteracting the pro-apoptotic activity of endogenous Xenopus Bid in egg extracts. Exogenously added recombinant xMcl-1 was rapidly degraded by proteasome in egg extracts, and we identified the destabilizing region in the N terminus of xMcl-1. Our results suggest that the proteolytic decay of xMcl-1 may change the functional balance between pro-and anti-apoptotic activities of Bcl-2 family proteins, thereby regulating the timing of cytochrome c release in egg extracts.

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“…We used the BM05, ID17 and ID19 beamlines of the European Synchrotron Radiation Facility (ESRF) to obtain data of the three studied species (Betz et al, 2007;Du Pasquier et al, 2007). We acquired tomographic data from two specimens of H. guentheri (energy of 25/52 Kev, with a propagation distance of 700/5000 mm and a voxel size of 5.04/30 μm), one P. kuhli (30 Kev, 862 mm, 40.48 μm) and one P. muralis (40 Kev, 900 mm, 14.93 μm).…”

Section: X-rays Synchrotron Propagation Phase Contrast Microtomographymentioning

confidence: 99%

Ecomorphological analysis of aerial performance in a non-specialized lacertid lizard,Holaspis guentheri

Vanhooydonck

Meulepas

Herrel

et al. 2009

Journal of Experimental Biology

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SUMMARYControlled aerial descent has evolved at least 30 times independently in different vertebrate and invertebrate lineages. A whole suite of morphological modifications, such as patagia, lateral skin folds and webbed feet, have been suggested to enhance descending ability. In this study, we compare aerial performance (i.e. vertical and horizontal velocity, horizontal distance covered, duration of descent) and morphology (body mass, body width, inter limb distance, surface area and wing loading) among three species of lizards, representing a range of aerial descenders present within the clade. Our performance measurements show that the lacertid Holaspis guentheri performs intermediately to the specialized gekkonid Ptychozoon kuhli and the rock-dwelling lizard Podarcis muralis. The small relative body mass of H. guentheri results in a low wing loading similar to that of P. kuhli thus enhancing its aerial performance. Whereas the latter generates great lift forces and is able to cover great horizontal distances, H. guentheri's low wing loading seems to be responsible for a slow descent and low impact forces upon landing. Our results show that very small morphological changes may result in noticeable and ecologically relevant changes in performance.

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TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis

Cited by 9 publications

References 45 publications

Assisted walking in Malagasy dwarf chamaeleons

Assisted walking in Malagasy dwarf chamaeleons

Anti-apoptotic Activity and Proteasome-mediated Degradation of Xenopus Mcl-1 Protein in Egg Extracts

Ecomorphological analysis of aerial performance in a non-specialized lacertid lizard,Holaspis guentheri

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