Improvement of the trace metal composition of medium for nitrite-dependent anaerobic methane oxidation bacteria: Iron (II) and copper (II) make a difference

He, Zhao; Geng, Sha; Pan, Yawei; Cai, Chaoyang; Wang, Jiaqi; Wang, Liqiao; Liu, Shuai; Zheng, Ping; Xu, Xinhua; Hu, Baolan

doi:10.1016/j.watres.2015.08.040

Cited by 59 publications

(16 citation statements)

References 47 publications

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“…In the last 6 months, the concentrations of the trace elements iron and copper (important components of the key enzymes in the central metabolism) in the medium were increased to 20 and 10 μM, respectively, to accelerate the growth of the methanotrophs32.…”

Section: Resultsmentioning

confidence: 99%

“…The alkaline trace element solution contained (per liter): 0.4 g NaOH, 0.242 g Na 2 MoO 4 ·2H 2 O, and 0.05 g Na 2 WO 4 ·2H 2 O. The acidic trace element solution was modified from previous literature532 and contained (per liter): 2.08–11.12 g FeSO 4 ·7H 2 O, 0.5–5 g CuSO 4 ·5H 2 O, 0.068 g ZnSO 4 ·7H 2 O, 0.12 g CoCl 2 ·6H 2 O, 0.5 g MnCl 2 ·4H 2 O, 0.095 g NiCl 2 ·6H 2 O, and 0.014 g H 3 BO 3 , and it was acidified to pH 1.0 by the addition of hydrochloric acid (HCl). The pH of the medium was adjusted to 7.2–7.4.…”

Section: Methodsmentioning

confidence: 99%

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A novel denitrifying methanotroph of the NC10 phylum and its microcolony

Cai

Wang

et al. 2016

Sci Rep

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The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich, and our knowledge regarding this phylum is largely limited to the first species ‘Candidatus Methylomirabilis oxyfera’ (M. oxyfera). Here, we enriched NC10 members from paddy soil and obtained a novel species of the NC10 phylum that mediates the anaerobic oxidation of methane (AOM) coupled to nitrite reduction. By comparing the new 16S rRNA gene sequences with those already in the database, this new species was found to be widely distributed in various habitats in China. Therefore, we tentatively named it ‘Candidatus Methylomirabilis sinica’ (M. sinica). Cells of M. sinica are roughly coccus-shaped (0.7–1.2 μm), distinct from M. oxyfera (rod-shaped; 0.25–0.5 × 0.8–1.1 μm). Notably, microscopic inspections revealed that M. sinica grew in honeycomb-shaped microcolonies, which was the first discovery of microcolony of the NC10 phylum. This finding opens the possibility to isolate NC10 members using microcolony-dependent isolation strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A novel denitrifying methanotroph of the NC10 phylum and its microcolony

Cai

Wang

et al. 2016

Sci Rep

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“…In the current system, oxygen is most likely not the cause of the separation into flocs and granules. Possibly, the formation of dense granules by N-damo bacteria and archaea prevents biomass washout (Luesken et al 2011a, 2011b; He et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Stultiens

Guerrero-Cruz

Kessel

et al. 2019

Appl Microbiol Biotechnol

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The reject water of anaerobic digestors still contains high levels of methane and ammonium that need to be treated before these effluents can be discharged to surface waters. Simultaneous anaerobic methane and ammonium oxidation performed by nitrate/nitrite-dependent anaerobic methane-oxidizing(N-damo) microorganisms and anaerobic ammonium-oxidizing(anammox) bacteria is considered a potential solution to this challenge. Here, a stable coculture of N-damo archaea, N-damo bacteria, and anammox bacteria was obtained in a sequencing batch reactor fed with methane, ammonium, and nitrite. Nitrite and ammonium removal rates of up to 455 mg N-NO 2 − L −1 day −1 and 228 mg N-NH 4 + L −1 were reached. All nitrate produced by anammox bacteria (57 mg N-NO 3 − L −1 day −1 ) was consumed, leading to a nitrogen removal efficiency of 97.5%. In the nitrite and ammonium limited state, N-damo and anammox bacteria each constituted about 30–40% of the culture and were separated as granules and flocs in later stages of the reactor operation. The N-damo archaea increased up to 20% and mainly resided in the granular biomass with their N-damo bacterial counterparts. About 70% of the nitrite in the reactor was removed via the anammox process, and batch assays confirmed that anammox activity in the reactor was close to its maximal potential activity. In contrast, activity of N-damo bacteria was much higher in batch, indicating that these bacteria were performing suboptimally in the sequencing batch reactor, and would probably be outcompeted by anammox bacteria if ammonium was supplied in excess. Together these results indicate that the combination of N-damo and anammox can be implemented for the removal of methane at the expense of nitrite and nitrate in future wastewater treatment systems.

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“…In previous studies, the mineral medium described by Raghoebarsing et al in 2006 has been used to enrich nitrite-and nitrate-dependent anaerobic methane-oxidizing microorganisms without significant changes (6). Recent studies have revealed that trace elements, such as Cu, Fe, and cerium (Ce), might be limiting in this medium (21,36,(44)(45)(46). While Cu and Fe are typical metals involved in many enzymes, Ce has only been recently recognized as an important metal for the XoxF-type methanol dehydrogenase (MDH) of methanotrophic and methylotrophic microorganisms, which employ Ce instead of Ca (44,47).…”

Section: Resultsmentioning

confidence: 99%

Key Physiology of a Nitrite-Dependent Methane-Oxidizing Enrichment Culture

Guerrero-Cruz

Stultiens

Kessel

et al. 2019

Appl Environ Microbiol

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Nitrite-dependent methane-oxidizing bacteria couple the reduction of nitrite to the oxidation of methane via a unique oxygen-producing pathway. This process is carried out by members of the genus Methylomirabilis that belong to the NC10 phylum. Contrary to other known anaerobic methane oxidizers, they do not employ the reverse methanogenesis pathway for methane activation but instead a canonical particulate methane monooxygenase similar to those used by aerobic methanotrophs. Methylomirabilis-like bacteria are detected in many natural and manmade ecosystems, but their physiology is not well understood. Here, using continuous cultivation techniques, batch activity assays, and state-of-the-art membrane-inlet mass spectrometry, we determined growth rate, doubling time, and methane and nitrite affinities of the nitrite-dependent methane-oxidizing bacterium "Candidatus Methylomirabilis lanthanidiphila." Our results provide insight into understanding the interactions of these microorganisms with methanotrophs and other nitrite-reducing microorganisms, such as anaerobic ammonium-oxidizing bacteria. Furthermore, our data can be used in modeling studies as well as wastewater treatment plant design. IMPORTANCE Methane is an important greenhouse gas with a radiative forcing 28 times that of carbon dioxide over a 100-year time scale. The emission of methane to the atmosphere is controlled by aerobic and anaerobic methanotrophs, which are microorganisms that are able to oxidize methane to conserve energy. While aerobic methanotrophs have been studied for over a century, knowledge on the physiological characteristics of anaerobic methanotrophs is scarce. Here, we describe kinetic properties of "Candidatus Methylomirabilis lanthanidiphila," a nitrite-dependent methane-oxidizing microorganism, which is ecologically important and can be applied in wastewater treatment.

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Improvement of the trace metal composition of medium for nitrite-dependent anaerobic methane oxidation bacteria: Iron (II) and copper (II) make a difference

Cited by 59 publications

References 47 publications

A novel denitrifying methanotroph of the NC10 phylum and its microcolony

A novel denitrifying methanotroph of the NC10 phylum and its microcolony

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Key Physiology of a Nitrite-Dependent Methane-Oxidizing Enrichment Culture

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