Key Physiology of a Nitrite-Dependent Methane-Oxidizing Enrichment Culture

Guerrero-Cruz, Simon; Stultiens, Karin; Kessel, Maartje A. H. J. van; Versantvoort, Wouter; Jetten, Mike S. M.; Camp, Huub J. M. Op den; Kartal, Boran

doi:10.1128/aem.00124-19

Cited by 42 publications

(27 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet, as in these previous studies, it was shown that in a mixed anammox/N-damo culture, the presence of Ca. Methylomirabilis-like bacteria in large numbers does not necessarily correspond to similar contributions to nitrite consumption (Luesken et al 2011a; Guerrero-Cruz et al 2019). Here, Ca.…”

Section: Discussionmentioning

confidence: 99%

“…Methanoperedens-like archaea can be cultured together in laboratory-scale membrane reactors, together removing methane, ammonium, nitrite, and nitrate from the influent (Ding et al 2017; Lu et al 2017; Shi et al 2013; Xie et al 2017a, b). In addition to cooperation, these organisms may also compete with each other under substrate limitation (Luesken et al 2011a, 2011b; Arshad et al 2017; Guerrero-Cruz et al 2019; Welte et al 2016). Ca.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Stultiens

Guerrero-Cruz

Kessel

et al. 2019

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

The reject water of anaerobic digestors still contains high levels of methane and ammonium that need to be treated before these effluents can be discharged to surface waters. Simultaneous anaerobic methane and ammonium oxidation performed by nitrate/nitrite-dependent anaerobic methane-oxidizing(N-damo) microorganisms and anaerobic ammonium-oxidizing(anammox) bacteria is considered a potential solution to this challenge. Here, a stable coculture of N-damo archaea, N-damo bacteria, and anammox bacteria was obtained in a sequencing batch reactor fed with methane, ammonium, and nitrite. Nitrite and ammonium removal rates of up to 455 mg N-NO 2 − L −1 day −1 and 228 mg N-NH 4 + L −1 were reached. All nitrate produced by anammox bacteria (57 mg N-NO 3 − L −1 day −1 ) was consumed, leading to a nitrogen removal efficiency of 97.5%. In the nitrite and ammonium limited state, N-damo and anammox bacteria each constituted about 30–40% of the culture and were separated as granules and flocs in later stages of the reactor operation. The N-damo archaea increased up to 20% and mainly resided in the granular biomass with their N-damo bacterial counterparts. About 70% of the nitrite in the reactor was removed via the anammox process, and batch assays confirmed that anammox activity in the reactor was close to its maximal potential activity. In contrast, activity of N-damo bacteria was much higher in batch, indicating that these bacteria were performing suboptimally in the sequencing batch reactor, and would probably be outcompeted by anammox bacteria if ammonium was supplied in excess. Together these results indicate that the combination of N-damo and anammox can be implemented for the removal of methane at the expense of nitrite and nitrate in future wastewater treatment systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Stultiens

Guerrero-Cruz

Kessel

et al. 2019

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…After 3 days, the air supply was turned off, and the culture was kept anoxic by continuous flushing with CH 4 /CO 2 (95%/5%, 10 mL min −1 ). The culture was fed with synthetic medium (Stultiens et al 2019) containing sufficient iron, copper, and cerium as trace elements, and was further supplemented with 5 mM NO 3 − , 0-8 mM NO 2 − , and 0.5-5 mM NH 4…”

Section: Batch Activity Testsmentioning

confidence: 99%

“…Oxygen is detrimental for all three processes (Strous et al 1999b;Luesken et al 2012;Guerrero-Cruz et al 2018). Although several studies have enriched combinations of Ndamo bacteria and archaea or N-damo archaea and anammox bacteria, few studies have been designed to enrich all three groups from the same source material (Arshad et al 2017;Stultiens et al 2019). In order to successfully start such timeconsuming long-term enrichment cultures, screening of the inoculum for the presence of markers.…”

Section: Introductionmentioning

confidence: 99%

“…This is supported by studies showing these anaerobic methane- and ammonium-oxidizing microorganisms living together in oxygen-limited ecosystems (reviewed in Welte et al 2016 ). In these ecosystems, the organisms may also have to compete with each other under substrate limitation (Luesken et al 2011a ; Winkler et al 2015 ; Arshad et al 2017 ; Guerrero-Cruz et al 2019 ). ‘ Ca .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

Stultiens

Kessel

Frank

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. After 14 months of operation, a stable enrichment culture containing two types of 'Candidatus Methylomirabilis oxyfera' bacteria and two strains of 'Ca. Brocadia'-like anammox bacteria was achieved. In this community, anammox bacteria converted 80% of the nitrite with ammonium, while 'Ca. Methylomirabilis' contributed to 20% of the nitrite consumption. The analysis of metagenomic 16S rRNA reads and fluorescence in situ hybridization (FISH) correlated well and showed that, after 14 months, 'Ca. Methylomirabilis' and anammox bacteria constituted approximately 30 and 20% of the total microbial community. In addition, a substantial part (10%) of the community consisted of Phycisphaera-related planctomycetes. Assembly and binning of the metagenomic sequences resulted in high-quality draft genome of two 'Ca. Methylomirabilis' species containing the marker genes pmoCAB, xoxF, and nirS and putative NO dismutase genes. The anammox draft genomes most closely related to 'Ca. Brocadia fulgida' included the marker genes hzsABC, hao, and hdh. Whole-reactor and batch anaerobic activity measurements with methane, ammonium, nitrite, and nitrate revealed an average anaerobic methane oxidation rate of 0.12 mmol h −1 L −1 and ammonium oxidation rate of 0.5 mmol h −1 L −1. Together, this study describes the enrichment and draft genomes of anaerobic methanotrophs from a brewery wastewater treatment plant, where these organisms together with anammox bacteria can contribute significantly to the removal of methane and ammonium in a more sustainable way. Key points • An enrichment culture containing both N-damo and anammox bacteria was obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth metagenomic biodiversity analysis of inoculum and enrichment culture.

show abstract

Substratum‐associated microbiota

Furey

Lee

Clemans

2020

Water Environment Research

View full text Add to dashboard Cite

Highlights of new, interesting, and emerging research findings on substratum-associated microbiota covered from a survey of 2019 literature from primarily freshwaters provide insight into research trends of interest to the Water Environment Federation and others interested in benthic, aquatic environments. Coverage of topics on bottom-associated or attached algae and cyanobacteria, though not comprehensive, includes new methods, taxa new-to-science, nutrient dynamics, auto-and heterotrophic interactions, grazers, bioassessment, herbicides and other pollutants, metal contaminants, and nuisance, and bloom-forming and harmful algae. Coverage of bacteria, also not comprehensive, focuses on the ecology of benthic biofilms and microbial communities, along with the ecology of microbes like Caulobacter crescentus, Rhodobacter, and other freshwater microbial species. Bacterial topics covered also include metagenomics and metatranscriptomics, toxins and pollutants, bacterial pathogens and bacteriophages, and bacterial physiology. Readers may use this literature review to learn about or renew their interest in the recent advances and discoveries regarding substratum-associated microbiota. © 2020 Water Environment Federation • Practitioner points • This review of literature from 2019 on substratum-associated microbiota presents highlights of findings on algae, cyanobacteria, and bacteria from primarily freshwaters. • Coverage of algae and cyanobacteria includes findings on new methods, taxa new to science, nutrient dynamics, auto-and heterotrophic interactions, grazers, bioassessment, herbicides and other pollutants, metal contaminants, and nuisance, bloomforming and harmful algae. • Coverage of bacteria includes findings on ecology of benthic biofilms and microbial communities, the ecology of microbes, metagenomics and metatranscriptomics, toxins and pollutants, bacterial pathogens and bacteriophages, and bacterial physiology. • Highlights of new, noteworthy and emerging topics build on those from 2018 and will be of relevance to the Water Environment Federation and others interested in benthic, aquatic environments

show abstract

Key Physiology of a Nitrite-Dependent Methane-Oxidizing Enrichment Culture

Cited by 42 publications

References 65 publications

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

Substratum‐associated microbiota

Contact Info

Product

Resources

About