Improvement of the production of subtilisin Carlsberg alkaline protease by Bacillus licheniformis by on-line process monitoring and control in a stirred tank reactor

Growth-rate-independent production of recombinant glucoamylase byFusarium venenatum JeRS 325

“…Several subtilisin-like alkaline proteases have been observed to be produced in a growth-correlated manner (Morita et al, 1994;van Putten et al, 1996). However, for other alkaline proteases, such as the trypsin-like proteases of Streptomyces exfoliatus (Kim and Lee, 1995) and Streptomyces albidoflavus (Kang and Lee, 1997), a negative correlation with specific growth rate has been observed.…”

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

“…Capacitance, T, pH, DO, dCO 2 E. coli van Steenkiste et al (1997) 5-50 mL OD, DO, pH, gene expression Recombinant E. coli Zanzotto et al (2004) 80-180 mL Growth rate Salmonella typhimurium Stitt et al (2002) 150 mL OD, DO pH E. coli; S. cerevisiae Zhang et al (2005) 200 mL D O Corynebacterium glutamicum John et al (2003b) 200 mL p H Lactococcus lactis; Streptococcus thermophilis John et al (2003a) 250 mL OD, pH, T E. coli Maharbiz et al (2004) 1 mL DO, pH E. coli Harms et al (2005) 2 mL OD, DO, pH E. coli Kostov et al (2001) 2 mL Cell density, pH Recombinant CHO and HEK Girard et al (2001) 2 mL Antibiotic Multiple cultures (mutation screening) Hammond and Brookes (1992) 6 mL OD, DO, pH, T E. coli Lamping et al (2003) 7 mL pH Saccharopolyspora erythraea Elmahdi et al (2003) Guarna et al (1997) pH and DO measured using fluorescent dyes, HPTS for pH and ruthenium-based for DO in various formats (e.g., phase modulation lifetime fluorimetry, solid state chips, in situ patch microfiber optic probes), as well as miniature and standard size probes for larger volumes (e.g., shake flasks). OD measured in situ using absorbance at 600 nm.…”

Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses‐based complex medium

“…Factors affecting enzyme production include the endogenus expression of the microorganism and inter-related bioreaction operation parameters such as fermentation medium composition, pH, 1,2 and oxygen transfer conditions, 3 -5 that contribute to the regulation of metabolic activity. Previous studies for SAP production using defined media have been reviewed byÇalık et al 6 Hübner et al 7 and van Putten et al 8 reported on SAP production using complex media, eg corn starch, Na-caseinate and corn steep liquor, and described on-line process monitoring techniques. Molasses is one of the most widely used complex substrates, but its use for SAP production has not been reported in depth in the published literature.…”