Growth-rate-independent production of recombinant glucoamylase byFusarium venenatum JeRS 325

Wiebe, Marilyn G.; Robson, Geoffrey D.; Shuster, J. R.; Trinci, Anthony P. J.

doi:10.1002/(sici)1097-0290(20000505)68:3<245::aid-bit2>3.0.co;2-f

Cited by 18 publications

(14 citation statements)

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“…it exhibits a low growth rate protein production phenotype [29-32]. This phenotype has been described in other Sordariomycetes [33], while high growth rate protein production has been described in Eurotiomycetes [34,35]. …”

Section: Introductionmentioning

confidence: 97%

Correlation of gene expression and protein production rate - a system wide study

et al. 2011

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BackgroundGrowth rate is a major determinant of intracellular function. However its effects can only be properly dissected with technically demanding chemostat cultivations in which it can be controlled. Recent work on Saccharomyces cerevisiae chemostat cultivations provided the first analysis on genome wide effects of growth rate. In this work we study the filamentous fungus Trichoderma reesei (Hypocrea jecorina) that is an industrial protein production host known for its exceptional protein secretion capability. Interestingly, it exhibits a low growth rate protein production phenotype.ResultsWe have used transcriptomics and proteomics to study the effect of growth rate and cell density on protein production in chemostat cultivations of T. reesei. Use of chemostat allowed control of growth rate and exact estimation of the extracellular specific protein production rate (SPPR). We find that major biosynthetic activities are all negatively correlated with SPPR. We also find that expression of many genes of secreted proteins and secondary metabolism, as well as various lineage specific, mostly unknown genes are positively correlated with SPPR. Finally, we enumerate possible regulators and regulatory mechanisms, arising from the data, for this response.ConclusionsBased on these results it appears that in low growth rate protein production energy is very efficiently used primarly for protein production. Also, we propose that flux through early glycolysis or the TCA cycle is a more fundamental determining factor than growth rate for low growth rate protein production and we propose a novel eukaryotic response to this i.e. the lineage specific response (LSR).

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Section: Introductionmentioning

confidence: 97%

Correlation of gene expression and protein production rate - a system wide study

et al. 2011

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“…However, examples of non growth associated production are also known, such as recombinant protein production by Fusarium venenatum [21]. Taking into account the data from the wide range of growth rate studies, the results in this study as well as those obtained by other scientists [3], [4], [15] indicate that production of the hydrolytic enzymes that constitute the major part of the extracellular proteins produced by T. reesei is not directly growth-rate associated.…”

Section: Discussionmentioning

confidence: 55%

High cell density cultivation of six fungal strains efficient in azo dye bioremediation

El‐Rahim

Mostafa

Moawad

2016

Biotechnology Reports

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HighlightsFungal strains having the potential of high cell density accumulation to be used for textile azo dyes bioremediation.The optimization of growth conditions for enhancing the biomass accumulation and protein production by six fungal strains was studied in 7 liter fermentor.The maximum biomass accumulation and protein production were achieved in molasses containing.The molasses as a cheaper carbon source was successfully used to promote fungal biomass and protein accumulation by the azo dye removing fungal strains.

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“…Shake flasks contained 50 ml modified Vogel's medium buffered to pH 4 or pH 5.5–6.0 with MES and were incubated on rotary shakers at 200 rpm (throw=2.5 cm). Batch and fed‐batch bioreactor cultures were grown in Braun Biostat M or FT Applikon bioreactors, as described by Wiebe et al [5]. Cultures were maintained at either pH 4.5 or 5.8±0.1, agitated at 1000 rpm, and aerated at ca 0.7 l air (l culture) −1 min −1 .…”

Section: Methodsmentioning

confidence: 99%

“…The promoter used to regulate production of these proteins was obtained from the F. oxysporum trypsin‐like protease, which is regulated independently from specific growth rate over a wide range (0.05–0.18 h −1 ) of specific growth rates [5]. Little or no recombinant protein is produced during exponential growth [5]. Additional copies of the A. niger GAM gene ( glaA ), under control of the A. niger glaA promoter, have been introduced into transformant JeRS 325, which produces GAM under control of the trypsin‐like protease promoter, in order to demonstrate the usefulness of the dual promoter strategy described above.…”

Section: Introductionmentioning

confidence: 99%

Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production inFusarium venenatum

Gordon

Thomas

Griffen

et al. 2001

FEMS Microbiology Letters

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Fusarium venenatum JeRS 325, a strain which produces recombinant glucoamylase under control of a growth rate independent promoter was transformed with a plasmid carrying the Aspergillus niger glucoamylase gene under control of its own growth rate correlated promoter. Some disruption of the original recombinant genes occurred and at pH 5.8 the double transformant did not produce as much glucoamylase as JeRS 325 in batch culture. However, the double transformant still produced as much glucoamylase as JeRS 325 in fed-batch cultures, illustrating the potential for the combined use of growth rate independent and dependent promoters to improve production of recombinant proteins in fed-batch culture systems.

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Growth-rate-independent production of recombinant glucoamylase byFusarium venenatum JeRS 325

Cited by 18 publications

References 34 publications

Correlation of gene expression and protein production rate - a system wide study

Correlation of gene expression and protein production rate - a system wide study

High cell density cultivation of six fungal strains efficient in azo dye bioremediation

Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production inFusarium venenatum

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