Improvement of the liver pathology by the aqueous extract and the n-butanol fraction of Sida pilosa Retz in Schistosoma mansoni-infected mice

Jatsa, Hermine Boukeng; Russo, Remo Castro; Pereira, Cíntia Aparecida de Jesus; Aguilar, Edenil Costa; Garcia, Cristiana C.; Araújo, Emília Souza; Oliveira, Jailza Lima Rodrigues; Rodrigues, Vanessa Fernandes; Oliveira, Vinícius Gustavo de; Alvarez-Leite, Jacqueline I.; Braga, Fernão Castro; Tchuenté, Louis-Albert Tchuem; Kamtchouing, Pièrre; Negrão-Corrêa, Déborah; Teixeira, Mauro Martins

doi:10.1016/j.jep.2016.01.017

Cited by 13 publications

(29 citation statements)

References 33 publications

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“…The dose of 200 mg/kg of SpAE was chosen for this study given that, in our previous study, it was one of the doses to display the best antioxidant, anti-inflammatory and anti-fibrotic activities [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, this fraction displayed good in vitro activity against S. mansoni [ 17 ]. However, in vivo studies on the evaluation of the schistosomicidal, antioxidant, anti-inflammatory and anti-fibrotic activities of S. pilosa aqueous extract and its n -butanol fraction has demonstrated that the aqueous extract was more active than the fraction [ 7 ]. The spasmogenic effect of Sida veronicaefolia (syn.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni – induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation

Jatsa

Femoe

Joseph

et al. 2018

BMC Complement Altern Med

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BackgroundThe macerate of Sida pilosa aerial parts is used empirically for the treatment of intestinal helminthiasis. Previous studies have shown that Sida pilosa aqueous extract (SpAE) has schistosomicidal, antioxidant, anti-inflammatory and anti-fibrotic activities in Schistosoma mansoni infection. This study was designed to evaluate the effect of SpAE on the granulomatous inflammation induced by S. mansoni in the liver and the intestine of mice by histomorphometry; as well as on the gastrointestinal motility.MethodsTo study the effect of SpAE on the liver and intestine histomorphometry and on the gastrointestinal motility, SpAE was administered at 200 mg/kg per os to S. mansoni-infected mice for 4 weeks. Praziquantel was used as reference drug. Prior to carrying out sacrifice, a batch of mice was subjected to gastrointestinal transit evaluation with 3% charcoal meal. After sacrifying another batch of mice, we performed histological and morphometric analyses of the liver and the ileum. We measured the following: total proteins, transaminases, malondialdehyde, nitrites, superoxide dismutase, catalase and reduced glutathione. The effect of SpAE (4, 8, 16 and 32 mg/mL) on the ileum contractile activity was evaluated either in the absence or in the presence of pharmacological blockers.ResultsSpAE induced a significant reduction of hepatosplenomegaly and intestine enlargement. The number of granulomas was reduced by 52.82% in the liver and 52.79% in the intestine, whereas the volume of hepatic granulomas decreased by 48.76% after SpAE treatment. SpAE also reduced (p < 0.001) the ileal muscular layer thickness. The levels of total proteins, transaminases, malondialdehyde, nitrites, superoxide dismutase, catalase and reduced glutathione were restored after treatment of infected mice with SpAE. A normalization of the gastrointestinal transit was also recorded after SpAE treatment. The effect of SpAE on intestinal motility was mediated via intracellular and extracellular calcium mobilization.ConclusionOur findings provide evidence that SpAE improves granulomatous inflammation induced by S. mansoni both in the liver and in the intestine, as well as it re-establishes normal gastrointestinal transit. SpAE may be used for the development of alternative medicine against S. mansoni infection.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni – induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation

Jatsa

Femoe

Joseph

et al. 2018

BMC Complement Altern Med

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“…Specie of the genus Sida have been reported to have a wide range of biological activities that include analgesic, anti-inflammatory, antioxidant, antibacterial and anthelmintic activities [16] [21]- [23]. Jatsa et al [12] have previously shown the in vivo schistosomicidal activity of S. pilosa aqueous extract against S. mansoni. With the perspective of searching new active compounds against Schistosoma species, the activity of S. pilosa aqueous extract and fractions thereof were assayed in vitro at different concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that the inhibition of larval migration, adult worm motility and egg excretion in helminths are associated with the consumption of tannins by their hosts [32]- [34]. It then appeared that tannins present in S. pilosa [12] could be involved in the reduction of worm motility and reproductive processes of schistosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies demonstrated the schistosomicidal activity of Sida pilosa aqueous extract using in vivo models. Moreover, phytochemical screening of S. pilosa aqueous extract revealed the presence of terpenoids, phenols, tannins and alkaloids [12]. This study was therefore designed to investigate the in vitro activity of S. pilosa aqueous extract and derived fractions towards Schistosoma mansoni.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni

Jatsa¹,

Pereira²,

Pereira³

et al. 2015

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Sida pilosa Retz. (Malvaceae) is a medicinal plant used in Africa for the treatment of dysmenorrhea, lower abdominal pains and intestinal helminthiasis. S. pilosa aqueous extract and derived fractions were investigated for their bioactivity against Schistosoma mansoni. The aqueous extract from S. pilosa aerial parts (1.25-40 mg/mL) and derived fractions (n-hexane, DCM, EtOAc and n-BuOH: 0.25-8 mg/mL) were tested on adult S. mansoni maintained in a GMEN culture medium. Praziquantel was used as the reference drug. After 24 h of incubation, worms were monitored for their viability and egg output. The antioxidant activity of S. pilosa was evaluated by the ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl free radicals. The chemical composition of the n-BuOH fraction was investigated by HPLC-MS analysis. S. pilosa aqueous extract and fractions significantly increased worm mortality in a concentration-dependent manner. The n-BuOH fraction was the most active with a LC50 of 1.25 mg/mL. Significant reduction of motor activity (25% to 100%) was * Corresponding author. H. B. Jatsa et al. 381 recorded for surviving worms incubated in different concentrations of the extract and fractions. Incubation of S. mansoni in different concentrations of S. pilosa extract and fractions led to significant reduction of egg laying (52% to 100%). The aqueous extract and derived fractions exhibited antioxidant activity in a concentration-dependent manner. The highest antioxidant activity was found with the EtOAc fraction, followed by the DCM and n-BuOH fractions. HPLC-MS analysis of the n-butanol fraction revealed the presence of two indoloquinoline alkaloids. This study disclosed the schistosomicidal activity of the n-butanol fraction from S. pilosa aqueous extract. This activity is probably related to the indoloquinoline alkaloids identified in the fraction.

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Immunomodulating and Anti-parasitary Mechanisms of Plant Products in the Treatment of Schistosomiasis

Lira,

Nogueira,

Licá

et al. 2023

Rev. Bras. Farmacogn.

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Improvement of the liver pathology by the aqueous extract and the n-butanol fraction of Sida pilosa Retz in Schistosoma mansoni-infected mice

Cited by 13 publications

References 33 publications

Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni – induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation

Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni – induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation

In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni

Immunomodulating and Anti-parasitary Mechanisms of Plant Products in the Treatment of Schistosomiasis

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