Improvement of recognition specificity of surface protein-imprinted magnetic microspheres by reducing nonspecific adsorption of competitors using 2-methacryloyloxyethyl phosphorylcholine

Li, Xiangjie; Zhang, Baoliang; Tian, Lei; Li, Wei; Zhang, Hepeng; Zhang, Qiuyu

doi:10.1016/j.snb.2014.11.045

Cited by 53 publications

(27 citation statements)

References 52 publications

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“…The intensity of MIP1 to MIP4 is lower compared with MIP5 which is lower than MIP6, where they exhibited the intensity of XRD to be lower than that of corresponding NIPs. The lower functional monomers and higher amount of crosslinker led to the formation of the optimized thin layer from the monomeric mixture of MIP composition (Figure S4, Supporting Information), resulting in higher selectivity . The results indicated the MIP4 has the optimum functional monomers, crosslinker and template molar ratio of 158:88:1 (M:C:T) and consequently minimum nonspecific binding after the polymerization process …”

Section: Resultsmentioning

confidence: 99%

“…The success of imprinting HSA in polymer is the ability to create optimized template cavities for target re‐inclusion. The variety of material surface for protein imprinting has been reported with high adsorption capacities of such as glass beads, silica beads, graphite materials, carbon nanotubes, nanowires, quantum dots, magnetic materials, and hydrogels . Hua et al .…”

Section: Introductionmentioning

confidence: 99%

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Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

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In this study, we report the development of adsorptive extraction materials by surface protein‐imprinted polymers (MIPs) over silica gel for selective recognition/separation of human serum albumin (HSA) from urine. The HSA‐imprinted polymers prepared on silica particle had at interface between the silica gel and different MIPs greatly produced enrichment for the binding of protein from the urine. The solid‐phase extraction of the optimized polymer layer was prepared by copolymerization of methacrylic acid (MAA), acrylamide (AAm), and dimethylaminoethylmethacrylate (DMAEMA) and a crosslinker methylenebisacrylamide (MBA) at the mole ratio of 1:158:88 (T:M:C) and showed moderate affinity (<104 order M−1) toward target protein HSA and selectivity. Four analogues, egg white albumin (EWA), bovine serum albumin (BSA), lysozyme (Lyz), and creatinine (Cre) were selected to study the binding efficiency of MIPs in single and binary protein solutions. We studied the influence on recognition ability for HSA and found that prepolymer mixture and matrix flexibility of the optimized thin polymer layer (35 ± 10 nm) on the submicrosilica particles. The high‐binding affinity (QMIP, 86.7 mg g−1) and fast kinetics (180 min) were observed for this synthesized HSA‐MIP when compared with other reported HSA‐MIPs in surface imprinting (5.9 and 11.3 mg g−1) and epitope surface imprinting (46.6 mg g−1) methods. We demonstrated the application in real and synthetic urine samples that the approach allowed the efficient adsorption of HSA in real urine (129.48 mg g−1) is almost double to the binding of HSA in synthetic urine (67.84 mg g−1). Apart from this, only minor interference of Cre (2.74 mg g−1) was observed, eventhough Cre is the final metabolite in urine. These adsorptive submicrosilica materials have potential in the pharmaceutical industry and clinical analysis applications. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 46894.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

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“…Therefore, this approach is well suited for imprinting of biomacromolecules like proteins . Depending on application modes, the solid supports subjected to surface imprinting of proteins can be nanomaterials , electrodes , microspheres , membranes , and monoliths. Molecular imprinting of proteins in polymers attached to the surface of nanomaterials is currently a hot topic; however, this application mainly focuses on the fabrication of biosensors since the nanomaterials exhibit a remarkably large surface‐to‐volume ratio and size‐related physical and chemical properties.…”

Section: Introductionmentioning

confidence: 99%

“Smart” molecularly imprinted monoliths for the selective capture and easy release of proteins

Wen

Tan

Sun

et al. 2016

J of Separation Science

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A new thermally switchable molecularly imprinted monolith for the selective capture and release of proteins has been designed. First, a generic poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith reacted with ethylenediamine followed by functionalization with 2-bromoisobutyryl bromide to introduce the initiator for atom transfer radical polymerization. Subsequently, a protein-imprinted poly(N-isopropylacrylamide) layer was grafted onto the surface of the monolithic matrix by atom transfer radical polymerization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy of the cross-sections of imprinted monoliths confirmed the formation of dense poly(N-isopropylacrylamide) brushes on the pore surface. The imprinted monolith exhibited high specificity and selectivity toward its template protein myoglobin over competing proteins and a remarkably large maximum adsorption capacity of 1641 mg/g. Moreover, this "smart" imprinted monolith featured thermally responsive characteristics that enabled selective capture and easy release of proteins triggered only by change in temperature with water as the mobile phase and avoided use of stronger organic solvents or change in ionic strength and pH.

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“…It was found that experimental data of adsorbing Lyz were well fitted to the pseudo-second-order model (r > 0.99) for both MCNTs@Lyz-MIPs and MCNTs@NIPs, indicating that chemical process was the rate-limiting step during the adsorption [32]. The v 0 of MCNTs@Lyz-MIPs was much higher than that of MCNTs@NIPs, illustrating the presence of specific binding sites on the surface of MCNTs@Lyz-MIPs.…”

Section: Adsorption Kineticsmentioning

confidence: 88%

Novel polydopamine imprinting layers coated magnetic carbon nanotubes for specific separation of lysozyme from egg white

Gao

Zhang

Hao

et al. 2015

Talanta

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Improvement of recognition specificity of surface protein-imprinted magnetic microspheres by reducing nonspecific adsorption of competitors using 2-methacryloyloxyethyl phosphorylcholine

Cited by 53 publications

References 52 publications

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

“Smart” molecularly imprinted monoliths for the selective capture and easy release of proteins

Novel polydopamine imprinting layers coated magnetic carbon nanotubes for specific separation of lysozyme from egg white

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