Improvement of Ram Sperm Cryopreservation Protocols Assessed by Sperm Quality Parameters and Heterogeneity Analysis

Ollero, Mario; Pérez‐Pé, Rosaura; Muiño‐Blanco, T.; Cebrián‐Pérez, J. A.

doi:10.1006/cryo.1998.2092

Cited by 66 publications

(40 citation statements)

References 47 publications

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“…The most important role of phospholipids could be coating the cell membrane, resulting in reinforcement during cryostorage. 80 Because the freezing/thawing process results in the loss of sperm surface hydrophobicity, 81 one of the proposed mechanisms of sperm resistance to cryostorage-induced stress is its ability to shed hydrophilic lipids, thus increasing the hydrophobic properties of the membrane and enhancing sperm tolerance to cryopreservation. 82 The exogenous addition of specific lipid compounds to the cell suspension can therefore be hypothesized to improve cell hydrophobicity and protect from cryopreservation-induced stress.…”

Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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“…The fatty acids are an essential requirement for the male germ cell to maintain sperm functions (Ollero et al 1998;Henkel 2005). ROS can also indirectly produce oxidative stress by decreasing the enzymatic defenses of the spermatozoa (Michael et al 2009).…”

Section: Tohoku University Medical Pressmentioning

confidence: 99%

Lycopene, an Antioxidant Carotenoid, Attenuates Testicular Injury Caused by Ischemia/Reperfusion in Rats

Hekimoğlu

Kurçer

Aral

et al. 2009

Tohoku J. Exp. Med.

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“…In recent years, the development of freezing techniques for semen cryopreservation has become a major resource for the preservation of genetic material in most domestic species (Songsasen & Leibo 1997a,1997b, Ollero et al 1998, Colenbrander et al 2003, Cremades et al 2005. Although the use of cryopreserved semen for artificial insemination (AI) has spread worldwide on a commercial basis for cattle (Curry et al 2000), this has not been the case for pigs, where extended semen in liquid form still dominates and !1% of frozen semen is used mostly in genetic nuclei or for export (Eriksson & RodriguezMartinez 2000).…”

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

Sancho

Casas²,

Ekwall³

et al. 2007

Reproduction

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This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the 'Entrepelado' and 'Lampiñ o' breeds. The samples were suspended in a commercial extender and refrigerated to 17 8C for transport to the laboratory (step A), where they were further extended with a lactose-egg yolk-based extender and chilled to 5 8C (step B) prior to freezing in the presence of glycerol (3%). Spermatozoa were assessed for plasma membrane integrity and sperm motility at each of the steps, including post-thaw (step C). Aliquots were also prepared for immunocytochemical localisation of the sugar transporters (fixed and thin smears for transmission and scanning electron microscopy levels respectively) and for SDS-PAGE electrophoresis and subsequent western blotting, using the same antibodies (rabbit anti-GLUT-3 and anti-GLUT-5 polyclonal antibodies). The results showed lower percentages of progressively motile spermatozoa at step C in both breeds, while the percentage of live spermatozoa was significantly lower only in the 'Entrepelado' breed. The results obtained from electron microscopy clearly showed that Iberian boar spermatozoa expressed the hexose transporters, GLUT-3 and GLUT-5. The pattern of expression, in terms of location and concentration, was characteristic in each case but, in the case of isoform GLUT-5, it remained constant during the different steps of freezing-thawing protocol. These results indicate that cryopreservation affects the status of sperm cells of Iberian boars by altering the distribution of some membrane receptors and decreasing the percentage values of parameters linked to sperm quality.

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Improvement of Ram Sperm Cryopreservation Protocols Assessed by Sperm Quality Parameters and Heterogeneity Analysis

Cited by 66 publications

References 47 publications

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Lycopene, an Antioxidant Carotenoid, Attenuates Testicular Injury Caused by Ischemia/Reperfusion in Rats

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

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