Improvement of quercetin protective effect against oxidative stress skin damages by incorporation in nanovesicles

Manca, Maria Letizia; Castangia, Inés; Caddeo, Carla; Pando, Daniel; Escribano, Elvira; Valenti, Donatella; Lampis, Sandrina; Zaru, Marco; Fadda, Anna Maria; Manconi, Maria

doi:10.1016/j.colsurfb.2014.09.059

Cited by 108 publications

(68 citation statements)

References 35 publications

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“…All the experiments were performed in triplicate. The percent antioxidant activity was calculated according to the following formula: antioxidant activity (%) = [(ABS DPPH −ABS sample )/ABS DPPH ]×100 [23].…”

Section: Antioxidant Activity Of Samples (Dpph Assay)mentioning

confidence: 99%

Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

et al. 2015

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Section: Antioxidant Activity Of Samples (Dpph Assay)mentioning

confidence: 99%

Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

et al. 2015

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“…An evaluation of the destabilization processes occurring in the colloidal dispersions was made by measuring the backscattering (BS) in the samples [25,26]. Figure 1C depicts the BS profiles for the vesicle dispersions: BS and time are shown on the ordinate, while the height of the cell is indicated on the abscissa of the graph.…”

Section: Vesicle Characterizationmentioning

confidence: 99%

“…In addition, the TurbiScan Lab ® Expert optical analyser equipped with an ageing station (Formulaction, Paris, France), was used to determine the accelerated stability of the vesicular dispersions, by monitoring the samples under realistic conditions (without mechanical stress or dilution) [25,26]. The instrument scans the entire height of the sample (λ = 880 nm), acquiring transmission and backscattering data every 40 µm, and shows the backscattering variation (∆BS) in the three sections (bottom, middle and top) of the cell.…”

Section: Stability Studymentioning

confidence: 99%

Faceted phospholipid vesicles tailored for the delivery of Santolina insularis essential oil to the skin

Castangia

Manca

Caddeo

et al. 2015

Colloids and Surfaces B: Biointerfaces

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The aim of this work was to formulate Santolina insularis essential oil-loaded nanocarriers, namely Penetration Enhancer containing Vesicles (PEVs), evaluate the physico-chemical features and stability, and gain insights into their ability to deliver the oil to the skin.Santolina insularis essential oil was obtained by steam distillation, and was predominantly composed of terpenes, the most abundant being β-phellandrene (22.6%), myrcene (11.4%) and curcumenes (12.1%). Vesicles were prepared using phosphatidylcholine, and ethylene or 2 propylene glycol were added to the water phase (10% v/v) to improve vesicle performances as delivery systems. Vesicles were deeply characterized by light scattering, cryogenic transmission electron microscopy and small/wide-angle X-ray scattering, the results showing polyhedral, faceted, unilamellar vesicles of ~115 nm in size. The presence of the glycols improved vesicle stability under accelerated ageing conditions, without changes in size or migration phenomena (e.g., sedimentation and creaming). Confocal laser scanning microscopy images of pig skin treated with Santolina insularis formulations displayed a penetration ability of PEVs greater than that of control liposomes. Moreover, all formulations showed a marked in vitro biocompatibility in human keratinocytes.These findings suggest that the nanoformulation may be of value in enhancing the delivery of Santolina insularis essential oil to the skin, where it can exert its biological activities.

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“…The same group showed that quercetin deformable liposomes increased the viability accompanied by decreased ROS and MDA in cells stimulated by UVB-irradiation. In another formulation, quercetin was entrapped in glycerosomes and liposomes to strengthen its antioxidant activity on human keratinocytes in presence of H 2 O 2 [36]. Quercetin was incorporated in different proportions into glycerosomes with 88-91% efficiency.…”

Section: Discussionmentioning

confidence: 99%

Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO2 Nanoparticles in Swiss 3T3 Albino Mouse Fibroblast Cells

Birinci¹,

Niazi²,

Başağa³

2017

J Nanomed Nanotechnol

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Living organisms are continuously exposed to a wide variety of oxidative radicals. Therefore, there is a constant demand for exogenous antioxidant supply. In this regard, one of these exogenous antioxidants with well established potency, quercetin has been utilized in various formulations. Yet due to its poor water solubility, its extensive applications are limited so far. In this study, TiO 2 nanoparticles (TiO 2 -NPs) were employed to improve the cellular penetration of quercetin and to maximize its antioxidant effects on swiss 3T3 albino mouse fibroblast cells. Surfaces of TiO 2 -NPs were modified with Polyethylene glycol (PEG) that enabled better dispersion and enhanced biocompatibility. Toxicity of quercetin and quercetin loaded TiO 2 NPs (QL-TiO 2 -NPs) were evaluated in terms of cell morphology and measured with cell viability assay. For an in-depth cell viability analysis, key markers of apoptosis were investigated by immunoblotting analysis. As an indicator of apoptotic cell death, cleavage of caspase 3 (Cas 3) and poly (ADP-ribose) polymerase proteins (Parp) were detected in quercetin treated cells. Antioxidant capacity of quercetin in the form of QL-TiO 2 -NPs was measured in the cells in which generation of reactive oxygen species (ROS) and superoxide was induced by pyocyanin. Quantitative ROS measurements were confirmed with confocal microscopy. Further mechanistic insight on upregulation of NF-E2 related factor 2 (NRF2) pathway via QL-TiO 2 -NPs was also provided in an effort to validate its antioxidant defense. Target enzymes of NRF2, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxydoreductase1 (NQO1) and superoxide dismutase1 (SOD1) expressions were increased in the model proposed. QL-TiO 2 -NPs allowed high bioavaliability of quercetin concentration and stability in the cell with maximum antioxidant capacity against formation of ROS without any cytotoxicity. Overall, this paper sheds light on how the efficiency of quercetin in a nanosystem can serve as a safe therapeutic candidate for breaking the vicious ROS cycle in the cell. Citation: Birinci Y, Niazi JH, Basaga H (2017) Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO 2 Nanoparticles in Swiss 3T3Albino Mouse Fibroblast Cells. J Nanomedic Nanotechnol S8: 001. doi:10.4172/2157-7439.S8-001Page 2 of 10 J Nanomedic Nanotechnol Nanotechnology: Challenges and Perspectives in Medicine ISSN: 2157-7439 JNMNT an open access journal 3T3 albino mouse fibroblast cells. Total cellular ROS and superoxide levels in treated cells were determined and compared with those of control cells. However, quercetin in a nanosystem shows maximum antioxidant activity by triggering NRF2 pathway, upregulating phase II antioxidant enzymes, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxydoreductase1 (NQO1) and superoxide dismutase1 (SOD1). In summary, our study demonstrated that quercetin loaded TiO 2 -NPs served as an effective nanosystem for delivering quercetin to swiss 3T3 albino mouse fibroblast cells in vitro and provided protection against oxida...

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Improvement of quercetin protective effect against oxidative stress skin damages by incorporation in nanovesicles

Cited by 108 publications

References 35 publications

Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

Faceted phospholipid vesicles tailored for the delivery of Santolina insularis essential oil to the skin

Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO2 Nanoparticles in Swiss 3T3 Albino Mouse Fibroblast Cells

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