Improvement of platelet storage conditions by using new polyolefin containers

J, de Wildt-Eggen; Jg, Schrijver; Hj, Bouter-Valk; Fijnheer, Rob; Bins, M.; Hc, van Prooijen

doi:10.1046/j.1537-2995.1997.37597293876.x

Cited by 42 publications

(52 citation statements)

References 23 publications

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“…Certainly, red cell storage studies showed the di(2-ethylhexyl) phthalate plasticizer helps maintain red cell viability during storage. 27,28 In vitro platelet assays suggest that the storage bag [29][30][31][32] and even the method of bag sterilization 33 may affect platelet quality during storage. When Terumo BCT platelets were stored in Haemonetics CLX bags, there were clear improvements in poststorage platelet viability, but the results may still not be as good as Haemonetics platelets stored in Haemonetics CLX bags (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

Slichter

Corson

Jones

et al. 2014

Blood

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• Extended apheresis platelet storage is dependent on the collection method, storage in a storage solution, and storage bag composition.• The lifespan of the platelet is not intrinsic to the cell, and platelet viability is better maintained in vitro than in vivo.To evaluate the poststorage viability of apheresis platelets stored for up to 18 days in 80% platelet additive solution (PAS)/20% plasma, 117 healthy subjects donated platelets using the Haemonetics MCS1, COBE Spectra (Spectra), or Trima Accel (Trima) systems. Control platelets from the same subjects were compared with their stored test PAS platelets by radiolabeling their stored and control platelets with either 51 chromium or 111 indium. Trima platelets met Food and Drug Administration poststorage platelet viability criteria for only 7 days vs almost 13 days for Haemonetics platelets; ie, platelet recoveries after these storage times averaged 44 6 3% vs 49 6 3% and survivals were 5.4 6 0.3 vs 4.6 6 0.3 days, respectively. The differences in storage duration are likely related to both the collection system and the storage bag. The Spectra and Trima platelets were hyperconcentrated during collection, and PAS was added, whereas the Haemonetics platelets were elutriated with PAS, which may have resulted in less collection injury. When Spectra and Trima platelets were stored in Haemonetics' bags, poststorage viability was significantly improved. Platelet viability is better maintained in vitro than in vivo, allowing substantial increases in platelet storage times. However, implementation will require resolution of potential bacterial overgrowth during storage. (Blood. 2014;123(2):271-280)

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Section: Discussionmentioning

confidence: 99%

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

Slichter

Corson

Jones

et al. 2014

Blood

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“…For sufficient supply of oxygen both the total surface and the gas permeability per unit of surface area of the container are important [6, 7, 8]. Thus, the total number of platelets that can be stored adequately in a container depends on the pO 2 and pCO 2 diffusion capacity.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the total number of platelets that can be stored adequately in a container depends on the pO 2 and pCO 2 diffusion capacity. When a certain maximum number is exceeded, platelet metabolism will switch to anaerobic glycolysis resulting in rapid acidification of the medium [6, 7, 8]. …”

Section: Introductionmentioning

confidence: 99%

“…Several studies [8, 9, 10, 11, 12]have indicated that storage of PCs with high yields in a single 1.0-liter PL732 container is not appropriate for maintenance of platelet quality. In a previous study [12]we demonstrated that adjustment of PCs in plasma (PCs-PL) platelet count to a maximum of 3.1 × 10 11 /U by reducing the volume, could prevent pH to drop below 6.8 in approximately 90% of the PCs-PL.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study [12]we demonstrated that adjustment of PCs in plasma (PCs-PL) platelet count to a maximum of 3.1 × 10 11 /U by reducing the volume, could prevent pH to drop below 6.8 in approximately 90% of the PCs-PL. In other studies [8, 9, 10, 11], all PCs either in plasma or platelet additive solutions (PAS) were stored in two PL 732 containers; in these studies pH values remained above 6.8. To be able to store PCs with higher yields, a 1.3-liter polyolefin platelet storage container (PL2410, Baxter Healthcare Corp.) with an increased permeability for oxygen and carbon dioxide in combination with increased surface area was recently developed.…”

Section: Introductionmentioning

confidence: 99%

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Platelets Stored in a New-Generation ContainerDifferences between Plasma and Platelet Additive Solution II

Wildt‐Eggen¹,

Schrijver²,

Smid³

et al. 1998

Vox Sanguinis

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Background and Objectives: In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelets in plasma and platelets additive solution. Materials and Methods: Platelet concentrate pools (PCs) of different yields suspended in either plasma (PCs-PL; n = 30) or PAS II (PCs-PAS; n = 37) were prepared. For preparation of PCs with a low, intermediate and high number of platelets 3, 5 and 6 buffy coat (BCs) were pooled with different volumes of plasma and 5 and 6 BCs were pooled with different volumes of PAS II, in order to obtain PCs of equal volumes comparable with routine conditions. All PCs were stored on a flatbed shaker at 22±2 °C and evaluated on days 1, 2, 5 and 7 by determining platelet and white cell counts, pH (37 °C), pO₂, pCO₂ and swirling score. Results: Platelet yields ranged from 1.5 up to 5.5 × 10¹¹ platelets/U. On day 7 all PCs-PL (n = 4) with platelet yields above 4.5 × 10¹¹/U had a pH value below 6.8 (range 5.91–6.79). While 7 of 8 PCs-PAS units with platelet yields above 4.0 × 10¹¹/U showed a pH value below 6.8 (range 6.31–6.70). Conclusion: During 7 days of storage in a new 1.3-liter platelet container, the pH was maintained above 6.8 in PCs in plasma with a yield between 1.5- and 4.5 × 10¹¹/U; when PAS II was used, the maximum platelet yield allowed for comparable pH maintenance was somewhat lower (4.0 × 10¹¹/U).

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Storage of single donor platelet concentrates: Paired comparison of storage as single or double concentrates

Glaser¹,

Friedlein²,

Zingsem³

et al. 2001

J of Clinical Apheresis

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Modern cell separators allow the collection of two plateletpheresis concentrates (PCs) at one session. This study evaluates the quality of PCs stored as double concentrates in standard storage containers of two manufacturers. We collected 20 PCs that contained 4.5 x 10(11) platelets in 375 ml plasma (10 using the COBE Spectra and 10 using the Fresenius AS.TEC 204 with 500 ml bags) that were split into one unit of 3.0 x 10(11) platelets in 250 ml (3.0-PC) and one of 1.5 x 10(11) platelets in 125 ml (1.5-PC). Storage of one 3.0-PC per bag of a two-bag system corresponded to storage conditions for double PCs and storage of one 1.5-PC per bag to storage conditions of single PCs. Cell counts, blood gas analysis, glucose and lactate levels, platelet aggregation, and activation and plasma levels of beta- thromboglobulin (beta-TG) and complement factor 3a (C3a) were measured before storage and again on days 3 and 5. COBE 3.0-PCs demonstrated less pH rise, lactate production, CD 62P expression and beta-TG plasma levels, and better aggregability after storage than COBE 1.5-PCs. Fresenius 1.5-PCs had similar platelet quality to COBE 3.0-PCs. Fresenius 3.0-PCs showed a fall of pH (day 5: 6.22 +/- 0.56), the highest amount of anaerobic glycolysis compared to all other storage conditions investigated, high CD 62P- expression and beta-TG plasma levels, and impaired aggregability on days 3 and 5. The highest C3a levels were found in COBE 1.5-PCs. 3.0 x 10(11) platelets in 250 ml plasma should be stored either in one bag of the COBE system or in two 500 ml bags of the Fresenius system. The COBE two-bag system allows the storage of two PCs without loss of platelet quality. Two PCs should not be stored in the Fresenius C4L 500 ml storage containers.

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Improvement of platelet storage conditions by using new polyolefin containers

Abstract: The new oxygen-permeable containers showed platelet quality comparable to that with the PL-732 and for longer storage periods and at higher platelet counts.

Cited by 42 publications

References 23 publications

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

Platelets Stored in a New-Generation ContainerDifferences between Plasma and Platelet Additive Solution II

Storage of single donor platelet concentrates: Paired comparison of storage as single or double concentrates

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