Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis

Rakariyatham, Nuansri; Butr-Indr, Bordin; Niamsup, Hataichanoke; Shank, Lalida

doi:10.2225/vol9-issue4-fulltext-13

Cited by 17 publications

(15 citation statements)

References 15 publications

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“…The supernatant was recovered and analyzed for myrosinase activity. Enzymatic activity was spectrophotometrically assessed through the method described by Rakariyatham, Butr-Indr, Niamsup, and Shank (2006). A 1 ml volume of substrate buffer (33 mM sodium phosphate buffer, pH 7.4 containing 10 mM sinigrin, 3 mM MgCl, 0.55 ATP, 0.72 mM NADP, 3.5 U hexokinase, and 1.75 U glucose-6-phosphate dehydrogenase) (Sigma-Aldrich, Schnelldorf, Germany) was pre-incubated for 3 min to 10 min at 30°C.…”

Section: Myrosinase Activitymentioning

confidence: 99%

Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars

et al. 2016

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Section: Myrosinase Activitymentioning

confidence: 99%

Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars

et al. 2016

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“…The supernatant was recovered. Enzyme activity was assessed spectrophotometrically through the method described by Rakariyatham et al (2006). One mL of substrate buffer (33 mM sodium phosphate buffer, pH 7.4 containing 10 mM sinigrin, 3 mM MgCl, 0.55 ATP, 0.72 mM NADP, 3.5 U hexokinase, and 1.75 U glucose-6-phosphate dehydrogenase) (Sigma-Aldrich, Schnelldorf, Germany) was pre-incubated for 3 min at 30°C.…”

Section: Myrosinase Activitymentioning

confidence: 99%

Optimization of an incubation step to maximize sulforaphane content in pre-processed broccoli

Mahn

Pérez

2016

J Food Sci Technol

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Sulforaphane is a powerful anticancer compound, found naturally in food, which comes from the hydrolysis of glucoraphanin, the main glucosinolate of broccoli. The aim of this work was to maximize sulforaphane content in broccoli by designing an incubation step after subjecting broccoli pieces to an optimized blanching step. Incubation was optimized through a BoxBehnken design using ascorbic acid concentration, incubation temperature and incubation time as factors. The optimal incubation conditions were 38°C for 3 h and 0.22 mg ascorbic acid per g fresh broccoli. The maximum sulforaphane concentration predicted by the model was 8.0 lmol g -1 , which was confirmed experimentally yielding a value of 8.1 ± 0.3 lmol g -1 . This represents a 585% increase with respect to fresh broccoli and a 119% increase in relation to blanched broccoli, equivalent to a conversion of 94% of glucoraphanin. The process proposed here allows maximizing sulforaphane content, thus avoiding artificial chemical synthesis. The compound could probably be isolated from broccoli, and may find application as nutraceutical or functional ingredient.

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“…The cultures were grown in defined medium with xylan as substrate, centrifuged (14 000 × g, 15 min, 5 °C), and exposed to different challenging doses of EtBr -1 (concentration 50, 100, and 150 mg mL ) (Chand et al 2005), and EMS (concentration 50, 100, and 150 mg -1 mL ) (Rakariyatham et al 2006), and incubated for 30, 60, 90, 120 min (Chand et al 2005). Cells surviving these doses were grown in agar medium containing 1% oat spelt xylan in the presence of (2% w/w from Oat spelt xylan) glucose, glycerol, or xylose.…”

Section: Mutagenesis and Screening Of Mutantsmentioning

confidence: 99%

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis

Hanim¹,

Yusiati²,

Cahyanto³

et al. 2013

Microbiol Indones

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This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wild-type counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence. Its optimum growth condition was also characterized and its phylogenetic relations to other xylanolytic bacteria were analyzed. Wild type xylanolytic alkalophlic bacteria were grown in medium containing xylan as a substrate. Mutation was performed using ethidium bromide (EtBr) or ethyl methanesulfonate (EMS) at concentrations 50, 100, and 150 mg mL-1 and times of exposure 30, 60, 90, and 120 min for each treatment. Twenty two mutants were obtained from EtBr and 24 mutants from EMS mutageneses. The mutants were analyzed for their capability to secrete xylanase into xylan medium containing xylose or glucose or glycerol. Growth optimizations of the mutant were done in media with pH range 6-11 and temperature range 30 to 60 °C. Mutant number 19, which was obtained by treatment using 50 mg mL-1 EMS for 120 min, had the highest xylanase activity (15.057 U g-1). This activity was obtained at optimum growth conditions: pH 9.5 and temperature 55 °C. Chromosomal DNA of this mutant was extracted and amplified by PCR using 16S rRNA gene specific primers. The amplified fragments were sequenced by dideoxynucleotide chain terminator method. The phylogenetic analysis based on 16S rRNA gene sequence showed that mutant 19 was closed to an anaerobic xylanase producing bacteria

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Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis

Cited by 17 publications

References 15 publications

Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars

Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars

Optimization of an incubation step to maximize sulforaphane content in pre-processed broccoli

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis

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