Improvement of mechanical properties of bio-concrete using Enterococcus faecalis and Bacillus cereus

Alshalif, Abdullah Faisal; Juki, Mohd Irwan; Othman, Norzila; Al-Gheethi, Adel; Khalid, Faisal Sheikh

doi:10.4491/eer.2018.306

Cited by 19 publications

(5 citation statements)

References 30 publications

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“…So, a repairing coating of calcium carbonate can behave as a natural calcite skin on the surface of bio-concrete (Achal et al, 2013). The observed reduction of water permeability in bio-concrete specimens reflects the action of the bacterial consortium representing a solution to one of the main problems concerning concrete durability (Alshalif et al, 2019). our results in agreement with several reports ( Luo et al, 2015;Tziviloglou et al, 2016;Balam et al 2017;Choi Sun-Gyu et al, 2017;Shashank and Nagaraja, 2021).…”

Section: Permeabilitysupporting

confidence: 91%

“…The surfaces of untreated concrete seemed to be amorphous, without obvious CaCO3 crystals appearance. In contrast, bio-concrete samples demonstrated crystalline surfaces where distinct CaCO3 crystals may recognize (Krishnapriya et al, 2015;Siddique et al, 2016;Alshalif et al, 2019). Reda et al, (2020) were reported that Bacillus flexus is the most potent one amongst the bacterial isolates that can form biofilm.…”

Section: Visual Observation Of Self-healingmentioning

confidence: 99%

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Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Mahmoud¹,

Kalaba

El-Sherbiny

et al. 2021

Preprint

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The small cracks in concrete constructions are inevitable due to deterioration during their service life throughout different load combination factors. In this study, we aimed to isolate, identify, and construct a bacterial consortium able to heal small cracks of concrete and enhance the different properties of concrete. Six isolates of bacillus, endospore-forming bacteria were isolated. There are only three isolates out of the six coded as NW-1, MK and NW-9 were showed the ability to produce urease enzyme and able to grow at 60°C with optimum growth at a temperature of 40°C. These isolates were survived in high pH, where isolate NW-1 was tolerated pH up to 11 with optimum growth at 10 while the isolates NW-9 and MK showed growth at pH 12 with an ideal growth at 10. CaCO3 production was observed by the three bacterial isolates whether in pure or mixed cultures (bacterial consortium) but the consortium consisting of MK and NW-9 was significantly the highest in productivity among them. Therefore, these two isolates were identified using 16s as Bacillus flexus MK-FYT-3 and Bacillus haynesii MK-NW-9 and deposited to GenBank under accession numbers MN965692 and MN965693 respectively. The effect of bacteria on some properties of concrete was studied, and the results showed that the compressive and tensile strengths of bio-concrete specimens were significantly increased by 31.29, 29 % after 7 days and 36.3, 39 % after 28 days of curing compared to control specimens. The results of permeability indicated that the bio-concrete specimens significantly showed less permeability than the control specimens by 21.1, 23.1% after 7 and 28 of curing, respectively. To determine the concrete density, Ultrasonic Pulse Velocity (UPV) test was performed, and the bio-concrete specimens gave higher values than control specimens by 26 and 20% after curing for 7 and 28 days, respectively. Also, surface healing of concrete was observed visually, the bio-concrete showed white precipitates around and inside the cracks after 7 days, which led to almost complete sealing of concrete after 28 days of curing, while the control samples were showed only very slight deposits on the surface and away from the cracks. The micro-analysis of concrete samples using SEM and XRD were done. It was found that the bio-concrete specimens showed crystalline precipitate with different shapes under SEM, while no such deposits appeared in the control specimens. On the other hand, the XRD profile was explained the characteristic peaks of calcium carbonate in both the bio-concrete and the control specimens, but the peak intensity was higher in the bio-concrete than the control specimens. This reflects the effectiveness of bacterial consortium in repairing and preventing the concrete cracks from spreading in addition to improving the various properties of concrete leading to increasing its life and sustainability.

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Section: Permeabilitysupporting

confidence: 91%

Section: Visual Observation Of Self-healingmentioning

confidence: 99%

Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Mahmoud¹,

Kalaba

El-Sherbiny

et al. 2021

Preprint

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“…Therefore, the surface of bioconcrete may have a healing layer of calcium carbonate that functions as a natural calcite skin [49]. As a response to one of the primary issues with concrete durability, the bacterial consortium's activity is reflected in the bio-concrete specimens' observed decrease in water permeability [50]. Several papers [51][52][53][54][55] concur with our findings.…”

Section: Absorption Of Water Test (Sorpativity Test)supporting

confidence: 84%

Manufacture and Study of Self-Healing Properties in Concrete With Bacillus Haynessii (Rakan1508) Bacteria Isolated From Farafra Oasis, New Vally Governorate, Egypt

Abbas,

Nooman,

Ismael

et al. 2024

Journal of Al-Azhar University Engineering Sector

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Concrete failure can be attributed to various factors, including cracks that allow chemical solutions to seep through this may have a substantial effect on Concrete Structures' Mechanical, Physical, and Durability Aspects. The idea that bones and tissues can repair themselves has been exploited, so we needed to make concrete that can self-heal cracks. The ability of a material to identify and mend internal damage without help from outside sources is known as self-healing. Our goal of this work was to identify, isolate, and create a bacterial consortia that could repair tiny concrete cracks and improve the concrete many qualities. Bacillus bacteria that generate endospores were discovered in four different isolates. Of the four isolates, only one was able to grow at 50°C and produce the urease enzyme; 40°C is the optimal temperature for growth. It also demonstrated growth potential at a pH of 12 with optimal growth at 10. Therefore, this isolate was identified using 16s as Bacillus haynesii RAKAN1508 and is stored in Gen-Bank with accession number OR642761. The progression of the healing ratio and the fracture profile were evaluated using both the scanning electron microscope with variable pressureCT scans using X-rays (also known as X-ray mCT). Additionally, this Studies were conducted to determine how bacteria affected properties concrete qualities. VP-SEM results showed that microbial precipitation fully healed a 0.4 mm crack mouth width, which XRD subsequently confirmed to be calcite and vaterite. Therefore, compared to control specimensthe ,bio-concrete specimens' compressive and tensile strengths increased dramatically by 36.3 and 44% following 28 days of curing. Per the sorpativity test results, which showed that after 28 days of healing, In contrast to the control samples, the bio-concrete specimens showed a substantial 23.1% decrease in permeability.The long-term repair of cracks in the concrete skin may be achieved with bacteria-based concrete self-healing.

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“…According to Rafat Siddique (2011), bacteria concentration is one of the most critical factors affecting the performance and compressive strength of bio-concrete [ 16 ]. While increasing the bacteria concentration increases compressive strength, exceeding the optimum value can have a negative effect [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Bio-Foamed Concrete Brick Strength via Bacteria Based Self-Healing and Bio-Sequestration of CO2

et al. 2021

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This research aimed to optimize the compressive strength of bio-foamed concrete brick (B-FCB) via a combination of the natural sequestration of CO2 and the bio-reaction of B. tequilensis enzymes. The experiments were guided by two optimization methods, namely, 2k factorial and response surface methodology (RSM). The 2k factorial analysis was carried out to screen the important factors; then, RSM analysis was performed to optimize the compressive strength of B-FCB. Four factors, namely, density (D), B. tequilensis concentration (B), temperature (T), and CO2 concentration, were selectively varied during the study. The optimum compressive strength of B-FCB was 8.22 MPa, as deduced from the following conditions: 10% CO2, 3 × 107 cell/mL of B, 27 °C of T and 1800 kg/m3 of D after 28 days. The use of B. tequilensis in B-FCB improved the compressive strength by 35.5% compared to the foamed concrete brick (FCB) after 28 days. A microstructure analysis by scanning electronic microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction analysis (XRD) reflected the changes in chemical element levels and calcium carbonate (CaCO3) precipitation in the B-FCB pores. This was due to the B. tequilensis surface reactions of carbonic anhydrase (CA) and urease enzyme with calcium in cement and sequestered CO2 during the curing time.

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Improvement of mechanical properties of bio-concrete using Enterococcus faecalis and Bacillus cereus

Cited by 19 publications

References 30 publications

Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Manufacture and Study of Self-Healing Properties in Concrete With Bacillus Haynessii (Rakan1508) Bacteria Isolated From Farafra Oasis, New Vally Governorate, Egypt

Optimization of Bio-Foamed Concrete Brick Strength via Bacteria Based Self-Healing and Bio-Sequestration of CO2

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