Improvement of kinetic properties and thermostability of recombinant Lepidium draba peroxidase (LDP) upon exposed to osmolytes

Sarvandi-Dehghanpoor, Elnaz; Riahi‐Madvar, Ali; Lotfi, Safa; Torkzadeh‐Mahani, Masoud

doi:10.1016/j.ijbiomac.2018.08.021

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…These changes may cause a variation in the enzyme. Changes in kinetic properties as a result of enzyme structure variation during drying has been reported previously [2][3][4][5][6] . Relative activity at each temperature indicates the ratio of the speci c peroxidase activity at a speci c temperature to the maximum speci c peroxidase activity which is the speci c peroxidase activity at 45°C 36 .…”

Section: Effect Of Drying On the Kinetics Of The Peroxidase Reactionmentioning

confidence: 62%

“…8, the thermal stability of the enzyme powder was improved during drying in the spouted bed dryer. Although the increase of enzyme thermal-stability was reported by several methods 2,35 but to the best of our knowledge, this is for the rst time that the enhancement of peroxidase thermalstability was reported by drying process. Shelf-life of the DEE during storage was also improved owing to drying in the spouted bed reactor, which is in agreement with the results of the enzyme drying in spray dryer 37 .…”

Section: Effect Of Drying On the Kinetics Of The Peroxidase Reactionmentioning

confidence: 78%

“…To determine the possible variation in enzyme thermal-stability, the DEE and FEE were incubated for 10 min at different temperature ranges (25-80 °C) and then after 5 min incubation at room temperature, enzyme activity was measured 2 . The shelf-life of DEE and FEE during storage was followed by determination of the changes in the enzyme activity during storage at -4 °C.…”

Section: Peroxidase Thermal-stability and Self-life During Storagementioning

confidence: 99%

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A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Hamedi

Afsahi

Riahi‐Madvar

2021

Preprint

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The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study for the first time, the spouted bed reactors were utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at the T= 50 °C and Q= 1.4. In order to investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that optimum pH was decreased about 12.3% while 85.7% increase was observed in optimum temperature of DEE compare to FEE. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.

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Section: Effect Of Drying On the Kinetics Of The Peroxidase Reactionmentioning

confidence: 62%

Section: Effect Of Drying On the Kinetics Of The Peroxidase Reactionmentioning

confidence: 78%

Section: Peroxidase Thermal-stability and Self-life During Storagementioning

confidence: 99%

See 1 more Smart Citation

A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Hamedi

Afsahi

Riahi‐Madvar

2021

Preprint

Self Cite

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show abstract

“…To determine the possible variation in enzyme thermal-stability, the DEE and FEE were incubated for 10 min at different temperature ranges (25–80 °C), and then after 5 min incubation at room temperature, enzyme activity was measured 2 . The shelf-life of DEE and FEE during storage was followed by the determination of the changes in the enzyme activity during storage at − 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…However, their sensitivity to heat, especially in the form of liquid, is a major application problem 1 . In this regard, various methods including the use of osmolytes 2 , mutagenesis 3 , immobilization 4 – 7 , and drying 8 have been developed to increase the thermal as well as the storage stability of the enzymes. One of the impressive and applicable methods for improving enzyme stability is enzyme drying.…”

Section: Introductionmentioning

confidence: 99%

Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

Hamedi

Afsahi

Riahi‐Madvar

et al. 2021

Sci Rep

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The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.

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Uncovering the role of sorbitol in Renilla luciferase kinetics: Insights from spectroscopic and molecular dynamics studies

Khoshnevisan,

Emamzadeh,

Nazari

et al. 2024

Biochemistry and Biophysics Reports

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Improvement of kinetic properties and thermostability of recombinant Lepidium draba peroxidase (LDP) upon exposed to osmolytes

Cited by 7 publications

References 48 publications

A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

Uncovering the role of sorbitol in Renilla luciferase kinetics: Insights from spectroscopic and molecular dynamics studies

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