Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer)

Yu, Kee-Won; Gao, Wenyuan; Son, Sung-Ho; Paek, Kee-Yoeup

doi:10.1007/s11627-000-0077-4

Cited by 92 publications

(51 citation statements)

References 23 publications

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“…Moreover, the intracellular signal cascade that begins with the interaction of an elicitor molecule with the plant cell surface results, ultimately, in the accumulation of secondary metabolites (Gundlach et al, 1992). The role of MJ as an endogenous elicitor for stimulating secondary metabolite production in cell suspensions, and hairy root cultures of various secondary plants has been reported, such as for the lignans pinoresinol, metarisinol (Schmitt and Petersen, 2002), saponin (Lu et al, 2001) and ginsenoside (Yu et al, 2000), and alkaloids (Zabetakis et al, 1999). In addition, Furmanowa and Syklowaka-Baranek (2000) reported that paclitaxel was accumulated at high levels in hairy root cultures of Taxus x media var.…”

Section: Resultsmentioning

confidence: 99%

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Saisavoey¹,

Thongchul²,

Sangvanich³

et al. 2014

J. Med. Plants Res.

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The effect of methyl jasmonate (MJ) on the isoflavonoid accumulation and activities of three antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were studied in Pueraria mirifica cell suspension culture after MJ elicitation for up to 6 days. Treatment with MJ at a concentration of 1.0 µg/ml induced the highest isoflavonoid contents at the first day after elicitation, when compared to the control. This optimum concentration of MJ induced an oxidative stress in P. mirifica cells. The activity levels of superoxide dismutase and glutathione peroxidase were both increased, whilst that for catalase was decreased in the MJ-treated cells. Thus, P. mirifica cells have a defense mechanism against superoxide and hydrogen peroxide stress and MJ is one such potential stimulator of this defense mechanism.

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Section: Resultsmentioning

confidence: 99%

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Saisavoey¹,

Thongchul²,

Sangvanich³

et al. 2014

J. Med. Plants Res.

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“…Lines selected for the evaluation of tenfold organic source of nitrogen (peptone from lactalbumin) showed different reactions depended additionally on mineral form of nitrogen used in media composition. Yu et al (2000) reported that peptone, but in lower (300 mg L −1 ) concentration, gave a slight enhancement of ginsenosides synthesis for Panax ginseng hairy roots. This effect was confirmed for the bioreactor culture of P. ginseng reported by the same group (Sivakumar et al 2005) without growth impairment.…”

Section: Discussionmentioning

confidence: 99%

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

2018

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Triterpenic saponins represented in Calendula officinalis L. by oleanolic acid (OA) glycosides are pentacyclic triterpene compounds with a wide range of biological and medicinal properties. This report demonstrates nitrogen source impact on growth, saponin accumulation, and secretion in hairy root and suspension cultures of marigold. Hairy roots preferred nitrate as a mineral source of nitrogen, but its impact on growth, OA glycosides accumulation, and secretion were line-dependent. The best productivity of OA glycosides was found in CC16 line (74.86 mg flask −1 ) in ½ MS medium modified by 2.5× KNO 3 and ammonium elimination with 2.5 g l −1 peptone. Organic nitrogen source at 27.5-g l −1 impairs the growth rate of hairy roots. Its effect on saponin accumulation and secretion to the surrounding medium depended on line and media composition. Nitrate:ammonium ratio of 4:2 for CC16 resulted in 5.7-fold increment of saponin secretion comparing to the standard medium. Embryo roots, apical bud, and hypocotyls explants were crucial for induction of suspension culture synthesizing saponins; however, effect of mineral form of nitrogen in cultivating medium had to be considered. The highest OA glycosides level (171.97 μg g −1 of dry weight) was recorded in the root derived culture with nitrate as a sole mineral form of nitrogen. Peptone from lactalbumin decidedly inhibited the saponin formation; however, it was essential for culture initiation, proliferation, and organ differentiation.

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“…It is difficult to clinically apply wild Korean mountain ginseng because of its scarcity and high cost. Recent advances in plant biotechnology have made it possible to produce mountain ginseng extracts on a large scale using adventitious root cultures in bioreactors [9]. In this study, we investigated the effects of mountain ginseng on male patients with ED using tissue-cultured mountain ginseng extract (TMGE).…”

Section: Introductionmentioning

confidence: 99%

Effects of tissue-cultured mountain ginseng (Panax ginseng CA Meyer) extract on male patients with erectile dysfunction

Kim¹,

Jeon²,

Hahn³

et al. 2009

Asian J Androl

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Korean ginseng and mountain ginseng (Panax ginseng CA Meyer) are important traditional herbal plants whose ginsenosides are generally accepted as serving to improve sexual functions, such as penile erection. We investigated the effects of tissue-cultured mountain ginseng extract (TMGE) on male patients with erectile dysfunction (ED). A double-blind, placebo-controlled study was conducted with 143 patients experiencing ED. Over the course of 8 weeks, one group took 1 000 mg of TMGE twice a day, and the other group took 1 000 mg of placebo twice a day. The effects of the TMGE and the placebo were analyzed using the Korean version of the International Index of Erectile Function (IIEF) questionnaire. A total of 86 patients completed 8 weeks of treatment. The scores on the five domains of the IIEF after medication were significantly higher than the baseline scores in the group treated with TMGE (P < 0.05), whereas no significant improvement was observed in the placebo group (P > 0.05). Erectile function and overall satisfaction scores after medication were significantly higher in the TMGE group than in the placebo group (P < 0.05). Erectile function of patients in the TMGE-treated group significantly improved, suggesting that TMGE could be utilized for improving erectile function in male patients.

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Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer)

Cited by 92 publications

References 23 publications

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

Effects of tissue-cultured mountain ginseng (Panax ginseng CA Meyer) extract on male patients with erectile dysfunction

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