Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization

El‐Enshasy, Hesham A.; Mohamed, Najya; Farid, Mohamed A.; El‐Diwany, Ahmed I.

doi:10.1016/j.biortech.2007.08.050

Cited by 50 publications

(28 citation statements)

References 26 publications

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“…154 In S. erythraea, the erythromycin biosynthetic cluster lacks a regulatory gene. 155 However, a 17.7-kDa bldD ortholog has recently been described, which positively regulates all promoters in the erythromycin production cluster, suggesting the existence of a transcriptional activator of the ery gene cluster.…”

Section: Streptomycesmentioning

confidence: 99%

Carbon source regulation of antibiotic production

et al. 2010

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Section: Streptomycesmentioning

confidence: 99%

Carbon source regulation of antibiotic production

et al. 2010

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“…Meanwhile, erythromycin productivity has also been enhanced by the optimization of fermentation process [7,8]. Since 1990s, genetic studies have provided insights into the genes involved in erythromycin biosynthesis, and the erythromycin gene cluster was found to contain 20 genes arranged in four major polycistronic units [9-11], which facilitated strain improvements by genetic and metabolic engineering [12-15].…”

Section: Introductionmentioning

confidence: 99%

Systems perspectives on erythromycin biosynthesis by comparative genomic and transcriptomic analyses of S. erythraea E3 and NRRL23338 strains

Chang

et al. 2013

BMC Genomics

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BackgroundS. erythraea is a Gram-positive filamentous bacterium used for the industrial-scale production of erythromycin A which is of high clinical importance. In this work, we sequenced the whole genome of a high-producing strain (E3) obtained by random mutagenesis and screening from the wild-type strain NRRL23338, and examined time-series expression profiles of both E3 and NRRL23338. Based on the genomic data and transcriptpmic data of these two strains, we carried out comparative analysis of high-producing strain and wild-type strain at both the genomic level and the transcriptomic level.ResultsWe observed a large number of genetic variants including 60 insertions, 46 deletions and 584 single nucleotide variations (SNV) in E3 in comparison with NRRL23338, and the analysis of time series transcriptomic data indicated that the genes involved in erythromycin biosynthesis and feeder pathways were significantly up-regulated during the 60 hours time-course. According to our data, BldD, a previously identified ery cluster regulator, did not show any positive correlations with the expression of ery cluster, suggesting the existence of alternative regulation mechanisms of erythromycin synthesis in S. erythraea. Several potential regulators were then proposed by integration analysis of genomic and transcriptomic data.ConclusionThis is a demonstration of the functional comparative genomics between an industrial S. erythraea strain and the wild-type strain. These findings help to understand the global regulation mechanisms of erythromycin biosynthesis in S. erythraea, providing useful clues for genetic and metabolic engineering in the future.

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“…Whereas, if the product is stable and could not be used as source of energy maintenance, cells will lyse accordingly. This observed significant reduction of biomass after carbon source limitation in bioreactor has been observed earlier also during the cultivation of other actinomycetes such as in Saccharopolyspora erythraea (El Enshasy et al 2008) and Amycolatopsis mediterranei (El Enshasy et al 2003). However, the rate of decrease in cell dry weight in the bioreactor was 0.025 g/L/h, which was almost the same value obtained in the shake flask culture under glucose limitation.…”

Section: Batch Cultivation Of S Rimosus In Stirred Tank Bioreactormentioning

confidence: 80%

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

Elsayed

Omar

El‐Enshasy

2015

Braz. arch. biol. technol.

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Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization

Cited by 50 publications

References 26 publications

Carbon source regulation of antibiotic production

Carbon source regulation of antibiotic production

Systems perspectives on erythromycin biosynthesis by comparative genomic and transcriptomic analyses of S. erythraea E3 and NRRL23338 strains

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

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