Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats

Abstract

BackgroundImpaired wound healing is a debilitating complication of diabetes that leads to significant morbidity, particularly foot ulcers. The risk of developing diabetic foot ulcers for diabetic patients is 15% over their lifetime and approximately 85% of limb amputations is caused by non–healing ulcers. Unhealed, gangrenous wounds destroy the structural integrity of the skin, which acts as a protective barrier that prevents the invasion of external noxious agents into the body. Vicenin-2 (VCN-2) has been reported to contain prospective anti-oxidant and anti-inflammatory properties that enhance cell proliferation and migration. Sodium Alginate (SA) is a natural polysaccharide that possesses gel forming properties and has biodegradable and biocompatible characteristics. Therefore, the objective of this study is to evaluate the effect of SA wound dressings containing VCN-2 on diabetic wounds.MethodsWounds were inflicted in type-1 diabetic-streptozotocin (STZ) induced male Sprague Dawley rats. Subsequently, relevant groups were topically treated with the indicated concentrations (12.5, 25 and 50 μM) of VCN-2 hydrocolloid film over the study duration (14 days). The control group was treated with vehicle dressing (blank or allantoin). Wounded tissues and blood serum were collected on 0, 7 and 14 days prior to sacrifice. Appropriate wound assessments such as histological tests, nitric oxide assays, enzyme-linked immunosorbent assays (ELISA) and immunoblotting assays were conducted to confirm wound healing efficacy in the in vivo model. One-way Analysis of Variance (ANOVA) was used for statistical analysis.ResultsResults showed that hydrocolloid film was recapitulated with VCN-2 enhanced diabetic wound healing in a dose-dependent manner. VCN-2 reduced pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α), mediators (iNOS and COX-2), and nitric oxide (NO) via the NF-κB pathway. Data suggests that the VCN-2 film facilitated healing in hyperglycemic conditions by releasing growth factors such as (VEGF and TGF-β) to enhance cell proliferation, migration, and wound contraction via the VEGF and TGF-β mechanism pathways.ConclusionsThis study’s findings suggest that VCN-2 may possess wound healing potential since topical treatment with VCN-2 hydrocolloid films effectively enhanced wound healing in hyperglycemic conditions.

Topical Wound Healing Activity of Myricetin Isolated from Tecomaria capensis v. aurea

“…These results have led us to suggest that myricetin accelerates the switch from inflammatory to anti-inflammatory responses, which promotes curing. These findings are consistent with other studies showing increased levels of TNF- α and IL-1 β in serum rats after a burn-injury [ 24 , 25 ].…”

Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing

“…It can be envisaged that the total aggregate effects of all these active components are unique and may act in synergism to accelerate wound and diabetic ulcer healing processes (Al-Hassan, 1990;Zheng et al, 2018). The current study paves the way for making potent therapeutic formulations with minimal number of stable active ingredients for wound healing (Gomes et al, 2017;Tan et al, 2019)or wound dressing applications (Zhao et al, 2017;Qu et al, 2018;.…”

Characterization of Alginate from Sargassum duplicatum and the Antioxidant Effect of Alginate–Okra Fruit Extracts Combination for Wound Healing on Diabetic Mice

“…In addition, active compounds pass penetration through the transappendageal route (sweat glands, hair follicles and sebaceous glands which include open channels on the outside surface of the skin) [57,58]. Tan et al [59] revealed that the topical application of Vicenin-2 film which is a type of flavonoid-derived glycosides from various natural plants can reduce blood-glucose levels in diabetic mice. Hence, that, lowering blood-glucose levels on diabetic mice can be influenced by topical treatment of open wounds.…”

“…The ointment formula contains flavonoids could be released into wounds and then to the bloodstream so as to induce secretion of cytokine, growth factor and increase insulin production or insulin sensitivity of somatic cells. Flavonoids also cause re-formation of antioxidants and serum lipid profiles in diabetic rats [59]. The wound healing activity of the okra fruit extract topically on open wounds effectively forms the complete epithelization on 15 day [72] and achieves a complete epithelization on 16 day [73].…”