Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing

Al-Hassan, Jassim M.; Hinek, Aleksander; Renno, Waleed M.; Wang, Yanting; Liu, Yuan Fang; Guan, Rui; Wen, Xiao-Yen; Litvack, Michael; Lindenmaier, Andras; Afzal, Muhammad; Paul, Bincy M.; Oommen, Sosamma; Nair, Divya; Kumar, Jijin; Khan, Meraj A.; Palaniyar, Nades; Pace-Asciak, Cecil R.

doi:10.3389/fphar.2020.00899

Cited by 10 publications

(21 citation statements)

References 57 publications

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“…The biochemically and pharmacologically active components of PCEGS are effective in a high percentage of the 75 cases of diabetic foot ulcers and 94 cases of different neurological disorders. PCEGS displayed potent anti-inflammatory and healing properties, as shown in early clinical trials, to heal non-healing diabetic foot ulcers, chronic back pain, and other neurological disorders (Al-Hassan 1990;Al-Hassan et al, 1991;Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007;Al-Hassan et al, 2020). These preliminary data have indicated that PCEGS had a noticeable effect on neuropathy in the diabetic foot and potent healing effects on the chronic back and joint pain (Al-Hassan 1990;Al-Hassan et al, 1991;Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007;Al-Hassan et al, 2020).…”

Section: Groupsmentioning

confidence: 99%

“…PCEGS displayed potent anti-inflammatory and healing properties, as shown in early clinical trials, to heal non-healing diabetic foot ulcers, chronic back pain, and other neurological disorders (Al-Hassan 1990;Al-Hassan et al, 1991;Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007;Al-Hassan et al, 2020). These preliminary data have indicated that PCEGS had a noticeable effect on neuropathy in the diabetic foot and potent healing effects on the chronic back and joint pain (Al-Hassan 1990;Al-Hassan et al, 1991;Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007;Al-Hassan et al, 2020). Further, PCEGS treatment completely healed the gangrenous feet, resulted in the natural degradation of the necrotic tissues, and some sensation returned to the neuropathic extremity of patients with non-healing foot ulcers or wet gangrene ulcers (Al-Hassan 1990).…”

Section: Groupsmentioning

confidence: 99%

“…The collected material was immediately frozen in dry ice and stored at −80°C until use. The PCEGS material was fractionated at 4°C to yield SPF-FB (Al-Hassan et al, 1982;Al-Hassan et al, 2020).…”

Section: Catfish (Arius Bilineatus Val) Epidermal Protein Preparationmentioning

confidence: 99%

“…Fractionation of SPF-FB From PCEGS From the Skin of the Catfish Fractionation of PCEGS to obtain soluble protein fraction, then Fraction B (SPF-FB) (United State Patent No. : US 10568915 B1 dated February 25, 2020) was carried out in Al-Hassan's laboratory and was provided for this project as a frozen mixture of proteins and lipids at −80°C (Al-Hassan et al, 1991;Pace-Asciak et al, 2017;Khan et al, 2019;Al-Hassan et al, 2020).…”

Section: Catfish (Arius Bilineatus Val) Epidermal Protein Preparationmentioning

confidence: 99%

“…The PCEGS lipid fraction (Ft-3) dose-dependently kills two cancer cell lines (breast MDA MB-231 and leukemic K-562) (Al-Hassan et al, 2019). Also, SPF-FB exhibited acceleration of wound healing (Pace-Asciak et al, 2017;Al-Hassan et al, 1991;Al-Hassan et al, 2020) potent anti-inflammatory and therapeutic activities, as shown by our previous clinical trials for the healing of non-healing diabetic foot ulcers (Al-Hassan 1990), chronic back pain, and other neurological disorders (Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007). These preliminary data have indicated that SPF-FB preparations had a noticeable effect on neuropathy in the diabetic foot and potent therapeutic effects on chronic back and joint pain (Al-Hassan, 1990;Al-Hassan et al, 2005;Al-Hassan et al, 2006;Al-Hassan et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Catfish Epidermal Preparation Accelerates Healing of Damaged Nerve in a Sciatic Nerve Crush Injury Rat Model

et al. 2021

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Preliminary investigations showed that preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) exhibit potent anti-inflammatory and healing properties as shown in our previous clinical trials for the healing of non-healing diabetic foot ulcers, chronic back pain, and some other neurological disorders. Here, we report for the first time a unique preparation containing only proteins and lipids (soluble protein fraction B, SPF-FB), derived from the PCEGS accelerated the healing and recovery of sensory-motor functions of experimental sciatic nerve crush injury in rats with its unique neuroprotective and neuroregenerative properties on the spinal neurons and peripheral nerve fibers. Male rats were randomly assigned to five groups: (I) NAÏVE, (II) SHAM, (III) CRUSH treated with saline, (IV) CRUSH + SPF-FB treated with 3 mg/kg intraperitoneally (IP) and (V) CRUSH + SPF-FB treated with 6 mg/kg subcutaneously (SC) groups. The crush groups III, IV and V underwent sciatic nerve crush injury, followed by treatment daily for 14 days with saline, SPF-FB IP and SPF-FB SC. All animals were tested for the neurobehavioral parameters throughout the 6 weeks of the study. Sciatic nerve and spinal cord tissues were processed for light and electron histological examinations, stereological analysis, immunohistochemical and biochemical examinations at Week 4 and Week 6 post-injury. Administration of SPF-FB IP or SC significantly enhanced the neurobehavioral sensory and motor performance and histomorphological neuroregeneration of the sciatic nerve-injured rats. The stereological evaluation of the axon area, average axon perimeters, and myelin thickness revealed significant histomorphological evidence of neuroregeneration in the FB-treated sciatic nerve crush injured groups compared to controls at 4 and 6 weeks. SPF-FB treatment significantly prevented the increased in NeuN-immunoreactive neurons, increased GFAP immunoreactive astrocytes, and decreased GAP-43. We conclude that SPF-FB treatment lessens neurobehavioral deficits, enhances axonal regeneration following nerve injury. We conclude that SPF-FB treatment lessens neurobehavioral deficits and enhances axonal regeneration following nerve injury, as well as protects spinal neurons and enhances subcellular recovery by increasing astrocytic activity and decreasing GAP-43 expression.

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Section: Groupsmentioning

confidence: 99%

Section: Groupsmentioning

confidence: 99%

Section: Catfish (Arius Bilineatus Val) Epidermal Protein Preparationmentioning

confidence: 99%

Section: Catfish (Arius Bilineatus Val) Epidermal Protein Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Catfish Epidermal Preparation Accelerates Healing of Damaged Nerve in a Sciatic Nerve Crush Injury Rat Model

et al. 2021

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Moschus ameliorates glutamate-induced cellular damage by regulating autophagy and apoptosis pathway

Xie,

Song,

Qin

et al. 2023

Sci Rep

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Alzheimer's disease (AD), a neurodegenerative disorder, causes short-term memory and cognition declines. It is estimated that one in three elderly people die from AD or other dementias. Chinese herbal medicine as a potential drug for treating AD has gained growing interest from many researchers. Moschus, a rare and valuable traditional Chinese animal medicine, was originally documented in Shennong Ben Cao Jing and recognized for its properties of reviving consciousness/resuscitation. Additionally, Moschus has the efficacy of “regulation of menstruation with blood activation, relief of swelling and pain” and is used for treating unconsciousness, stroke, coma, and cerebrovascular diseases. However, it is uncertain whether Moschus has any protective effect on AD patients. We explored whether Moschus could protect glutamate (Glu)-induced PC12 cells from cellular injury and preliminarily explored their related action mechanisms. The chemical compounds of Moschus were analyzed and identified by GC–MS. The Glu-induced differentiated PC12 cell model was thought to be the common AD cellular model. The study aims to preliminarily investigate the intervention effect of Moschus on Glu-induced PC12 cell damage as well as their related action mechanisms. Cell viability, lactate dehydrogenase (LDH), mitochondrial reactive oxygen species, mitochondrial membrane potential (MMP), cell apoptosis, autophagic vacuoles, autolysosomes or autophagosomes, proteins related to apoptosis, and the proteins related to autophagy were examined and analyzed. Seventeen active compounds of the Moschus sample were identified based on GC–MS analysis. In comparison to the control group, Glu stimulation increased cell viability loss, LDH release, mitochondrial damage, loss of MMP, apoptosis rate, and the number of cells containing autophagic vacuoles, and autolysosomes or autophagosomes, while these results were decreased after the pretreatment with Moschus and 3-methyladenine (3-MA). Furthermore, Glu stimulation significantly increased cleaved caspase-3, Beclin1, and LC3II protein expression, and reduced B-cell lymphoma 2/BAX ratio and p62 protein expression, but these results were reversed after pretreatment of Moschus and 3-MA. Moschus has protective activity in Glu-induced PC12 cell injury, and the potential mechanism might involve the regulation of autophagy and apoptosis. Our study may promote research on Moschus in the field of neurodegenerative diseases, and Moschus may be considered as a potential therapeutic agent for AD.

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Cross-linked chitosan/lysozyme hydrogels with inherent antibacterial activity and tuneable drug release properties for cutaneous drug administration

Lai

Obireddy

Zhang

et al. 2023

Science and Technology of Advanced Materials

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Gels with high drug release sustainability and intrinsic antibacterial properties are of high practical potential for cutaneous drug administration, particularly for wound care and skin disease treatment. This study reports the generation and characterization of gels formed by 1,5-pentanedial-mediated crosslinking between chitosan and lysozyme for cutaneous drug delivery. Structures of the gels are characterized by using scanning electron microscopy, X-ray diffractometry and Fourier-transform infrared spectroscopy. An increase in the mass percentage of lysozyme leads to an increase in the swelling ratio and erosion susceptibility of the resulting gels. The drug delivery performance of the gels can be changed simply by manipulating the chitosan/lysozyme mass-to-mass ratio, with an increase in the mass percentage of lysozyme leading to a decline in the encapsulation efficiency and drug release sustainability of the gels. Not only do all gels tested in this study show negligible toxicity in NIH/3T3 fibroblasts, they also demonstrate intrinsic antibacterial effects against both Gram-negative and Gram-positive bacteria, with the magnitude of the effect being positively related to the mass percentage of lysozyme. All these warrant the gels to be further developed as intrinsically antibacterial carriers for cutaneous drug administration.

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Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing

Cited by 10 publications

References 57 publications

Catfish Epidermal Preparation Accelerates Healing of Damaged Nerve in a Sciatic Nerve Crush Injury Rat Model

Catfish Epidermal Preparation Accelerates Healing of Damaged Nerve in a Sciatic Nerve Crush Injury Rat Model

Moschus ameliorates glutamate-induced cellular damage by regulating autophagy and apoptosis pathway

Cross-linked chitosan/lysozyme hydrogels with inherent antibacterial activity and tuneable drug release properties for cutaneous drug administration

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