Improvement of delivery and live birth rates after ICSI in women aged &gt;40 years by ovarian co-stimulation with growth hormone

Hazout, A.; Mendoza, Carmen

doi:10.1093/humrep/dei066

Cited by 112 publications

(118 citation statements)

References 44 publications

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“…**P!0.005 and ***P!0.001. demonstrated using 8 IU daily for a short interval; from day 7 for 6-8 days to the day after Trigger (Tesarik et al 2005) along with our studies where the Saizen regimen produced comparable improvements (Yovich & Stanger 2010). The SciTropin A regimen has also shown equivalent clinical benefits in other study (JL Yovich and J Stanger, unpublished observations).…”

Section: Discussionsupporting

confidence: 78%

A direct action for GH in improvement of oocyte quality in poor-responder patients

Weall¹,

Al-Samerria²,

Conceicao³

et al. 2015

Reproduction

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Declining female fecundity at later age and the increasing tendency for women to delay childbirth have lead to a drastic rise in the number of women seeking assisted reproductive technology. Many women fail to respond adequately to standard ovarian stimulation regimens, raising a significant therapeutic challenge. Recently, we have demonstrated that the administration of GH, as an adjunct to ovarian stimulation, has improved the clinical outcomes by enhancing the oocyte quality. However, the mechanism(s) by which GH facilitated this improvement is yet to be understood. This study aimed to determine these potential mechanism(s) through the use of immunofluorescent localisation of GH receptors (GHRs) on the human oocyte and unbiased computer-based quantification to assess and compare oocyte quality between women of varying ages, with or without GH treatment. This study demonstrates for the first time, the presence of GHRs on the human oocyte. The oocytes retrieved from older women showed significant decrease in the expression of GHRs and amount of functional mitochondria when compared with those from younger patients. More interestingly, when older patients were treated with GH, a significant increase in functional mitochondria was observed in their oocytes. We conclude that GH exerts a direct mode of action, enabling the improvement of oocyte quality observed in our previous study, via the upregulation of its own receptors and enhancement of mitochondrial activity. This result, together with recent observations, provides scientific evidence in support of the use of GH supplementation for the clinical management of poor ovarian response.

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Section: Discussionsupporting

confidence: 78%

A direct action for GH in improvement of oocyte quality in poor-responder patients

Weall¹,

Al-Samerria²,

Conceicao³

et al. 2015

Reproduction

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“…A recent Cochrane Library review found a significant improvement in live birth rate in poor responders despite no effect in normal responders [8]. In a recent randomized controlled trial [9], it has been reported similar number of oocytes, embryos and pregnancies but improvement of delivery and live birth rates after ICSI in 50 women older than 40 years old by ovarian co-stimulation with growth hormone. The pre-conditioning of early antral follicles with the use of growth hormone was never reported before.…”

Section: Introductionmentioning

confidence: 99%

Growth hormone co-treatment within a GnRH agonist long protocol in patients with poor ovarian response: a prospective, randomized, clinical trial

Küçük

Kozinoglu²,

Kaba³

2008

J Assist Reprod Genet

103

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“…Our observation confirms that GH can be used successfully to mature GV oocytes in a culture medium containing the patient serum, with no extra hormone addition. A role for GH is possible in vivo during human oocyte maturation (4). GH effects on the oocytes can be direct and not necessarily through the cumulus cells even if the GHR mRNA in abundant in cumulus cells (1).…”

Section: Resultsmentioning

confidence: 99%

Pregnancy and delivery after in vitro maturation of naked ICSI GV oocytes with GH and transfer of a frozen thawed blastocyst: case report

Ménézo¹,

Nicollet²,

Rollet³

et al. 2006

J Assist Reprod Genet

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Purpose:To determine if GV oocytes, collected at the time of ICSI, can be matured in vitro and rescued for therapeutic treatment. A patient for whom all the collected oocytes at the GV stage after a classical COH protocol were matured in vitro with GH. Method: All the naked oocytes were matured in a culture medium (ISM2) containing 15% patient serum +1.6 units of GH (Saizen) per millilitre. Oocytes were incubated overnight at 37 • C. The MII oocytes obtained were micro-injected. A fresh transfer was performed and a supernumerary blastocyst was frozen. Results: The patient was pregnant and delivered a healthy girl after transfer of the frozen/thawed blastocyst. The baby girl is now 2 years old. Conclusion: In vitro maturation with GH allows rescuing naked GV oocytes collected at the time of ICSI. GH action does not pass through the cumulus cells. According to the possible lack of synchrony between the embryo and the uterus, we recommend to freeze the embryos obtained and to replace them in a controlled cycle.

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Improvement of delivery and live birth rates after ICSI in women aged >40 years by ovarian co-stimulation with growth hormone

Abstract: Administration of GH during ovarian stimulation alleviates age-related decrease in assisted reproduction treatment efficiency. This effect appears to be mainly due to an improvement of oocyte developmental potential, but GH action on the uterus cannot be excluded.

Cited by 112 publications

References 44 publications

A direct action for GH in improvement of oocyte quality in poor-responder patients

A direct action for GH in improvement of oocyte quality in poor-responder patients

Growth hormone co-treatment within a GnRH agonist long protocol in patients with poor ovarian response: a prospective, randomized, clinical trial

Pregnancy and delivery after in vitro maturation of naked ICSI GV oocytes with GH and transfer of a frozen thawed blastocyst: case report

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