Improvement of cooled equine semen by addition of carnitines

Lisboa, Fernando Paixão; Hartwig, F.P.; Freitas-Dell’Aqua, C.P.; Papa, Frederico Ozanam; Dell’Aqua, J.A.

doi:10.1016/j.jevs.2013.10.028

Cited by 9 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its antioxidant properties that protect sperm membranes against toxic reactive oxygen species (ROS) are mostly related to the transfer of the products of β -oxidation to the mitochondria for oxidation to CO 2 and H 2 O in the Krebs cycle [ 40 ]. Recent studies have shown that the in vitro addition of L-carnitine in spermatozoa improves their viability and motility [ 22 , 27 – 29 ]. However, in the current study, an improvement in sperm motility was recorded when LC was not added following cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…Several human studies have shown their positive effect on sperm motility and viability [ 22 – 26 ]. Moreover, animal studies revealed that the in vitro addition of these antioxidants improved sperm quality [ 27 – 31 ]. However, to the best of our knowledge, there are no data available about comparison of the effect of L-carnitine, hypotaurine, and taurine on sperm characteristics in cryopreserved chicken semen.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Partyka

Rodak

Bajzert

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P < 0.01 and P < 0.05) following cryopreservation. After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control (P < 0.05). There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments (P < 0.01; P < 0.05), when compared to the control group. The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 (P < 0.05). Supplementation of the diluent with LC1, LC5, and T1 significantly (P < 0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups. These results indicate that the addition of examined antioxidants improves the quality of cryopreserved chicken semen.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Partyka

Rodak

Bajzert

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…While there are numerous reports of beneficial effects of oral L-C administration on sperm parameters of horses [27] and other species [44,46,[49][50][51][52][53][54][55][56], there are notably few publications investigating the effect of in vitro supplementation of L-C on stallion spermatozoa [57,58]. L-C is powerful osmolyte, and therefore its addition to media in this study necessitated the partial removal of NaCl to maintain isotonicity.…”

Section: Figmentioning

confidence: 99%

L-Carnitine and Pyruvate Are Prosurvival Factors During the Storage of Stallion Spermatozoa at Room Temperature1

Gibb

Lambourne

Quadrelli

et al. 2015

Biology of Reproduction

View full text Add to dashboard Cite

The spermatozoa of many stallions do not tolerate being cooled, restricting the commercial viability of these animals and necessitating the development of a chemically defined room temperature (RT) storage medium. This study examined the impact of two major modulators of oxidative phosphorylation, pyruvate (Pyr) and L-carnitine (L-C), on the storage of stallion spermatozoa at RT. Optimal concentrations of Pyr (10 mM) and L-C (50 mM) were first identified and these concentrations were then used to investigate the effects of these compounds on sperm functionality and oxidative stress at RT. Mitochondrial and cytosolic reactive oxygen species, along with lipid peroxidation, were all significantly suppressed by the addition of L-C (48 h MitoSOX Red negative: 46.2% vs. 26.1%; 48 and 72 h dihydroethidium negative: 61.6% vs. 43.1% and 64.4% vs. 46.9%, respectively; 48 and 72 h 4-hydroxynonenal negative: 37.1% vs. 23.8% and 41.6% vs. 25.7%, respectively), while the Pyr + L-C combination resulted in significantly higher motility compared to the control at 72 h (total motility: 64.2% vs. 39.4%; progressive motility: 34.2% vs. 15.2%). In addition, supplementation with L-C significantly reduced oxidative DNA damage at 72 h (9.0% vs. 15.6%). To investigate the effects of L-C as an osmolyte, comparisons were made between media that were osmotically balanced with NaCl, choline chloride, or L-C. This analysis demonstrated that spermatozoa stored in the L-C balanced medium had significantly higher total motility (55.0% vs. 39.0%), rapid motility (44.0% vs. 25.7%), and ATP levels (70.9 vs. 12.8 ng/ml) following storage compared with the NaCl treatment, while choline chloride did not significantly improve these parameters compared to the control. Finally, mass spectrometry was used to demonstrate that a combination of Pyr and L-C produced significantly higher acetyl-L-carnitine production than any other treatment (6.7 pg/10(6) spermatozoa vs. control at 4.0 pg/10(6) spermatozoa). These findings suggest that Pyr and L-C could form the basis of a novel, effective RT storage medium for equine spermatozoa.

show abstract

“…17 However, studies investigating the effect of supplementation in vitro with LC during equine sperm cooling are limited. 18,19 These features suggest that LC plays an important role in sperm fertility. Therefore, the objectives of this study were to investigate the relatioship of seminal and serum levels of LC on sperm function, as well as to evaluate the effects of LC added to a skimmed milk-based extender on horse sperm during storage at 5°C.…”

Section: Introductionmentioning

confidence: 99%

Effects of L-Carnitine on Equine Semen Quality During Liquid Storage

Nery

Silva

Souza

et al. 2020

Biopreservation and Biobanking

View full text Add to dashboard Cite

l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through b-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based extender during sperm storage at 5°C. In the first experiment, semen and blood samples from 14 Quarter Horse stallions were used. The LC content in blood plasma and seminal plasma was determined by spectrophotometry and their relationships with seminal parameters were evaluated. In the second experiment, ejaculates (n = 16) from four Quarter Horses were used. Each ejaculate was split into four treatment groups with different LC concentrations: 0 (control), 0.5, 1.0, and 2.0 mM. Sperm motility, integrity of plasma and acrosomal membranes, intracellular reactive oxygen species content, and plasma membrane stability were evaluated immediately after samples reached 5°C (0 hour) and after 24, 48, and 72 hours. There was a positive correlation ( p < 0.05) between LC levels in seminal plasma with both sperm concentration and plasma and acrosomal membrane integrity. Furthermore, the addition of LC (1 and 2 mM) preserved the motility of equine sperm stored at 5°C. It was concluded that the concentrations of LC with seminal plasma present correlate to semen parameters and the addition of LC to skimmed milk-based extender preserves the motility of equine sperm stored at 5°C for up to 48 hours.

show abstract

Improvement of cooled equine semen by addition of carnitines

Cited by 9 publications

References 0 publications

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

L-Carnitine and Pyruvate Are Prosurvival Factors During the Storage of Stallion Spermatozoa at Room Temperature1

Effects of L-Carnitine on Equine Semen Quality During Liquid Storage

Contact Info

Product

Resources

About