Improvement of Clonal Micropropagation of Berry Crops

Markova, M. G.; Somova, E. N.

doi:10.12737/2073-0462-2021-39-44

Cited by 3 publications

(4 citation statements)

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“…All experiments were conducted in the Laboratory of Plant Biotechnology at Tsitsin Main Botanical Garden of the Russian Academy of Sciences (MBG RAS), Moscow, Russia. The generally accepted methods of plant biotechnology (Butenko, 1964) developed in the Laboratory (Molkanova et al, 2018) were used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…Some studies showed that the determination of the morphogenetic potential of most taxa mostly depends on the genetic traits of the plants (Mitrofanova, 2018;Molkanova et al, 2018;Marino;Bertazza, 1990). The regeneration capacity of the explants of different Actinidia species varied significantly; A. arguta and A. polygama developed faster than A. kolomikta at the micropropagation stage (Figure 1), which correlated with their growth dynamics during introduction.…”

Section: Shoot Multiplicationmentioning

confidence: 98%

“…While optimizing the clonal micropropagation technique, the biological traits and details of the in vitro regeneration of each taxon must be considered. They depend on several factors, which include the genotype, epigenetic traits of explant cells, the physiological state of intact plants, the composition of the medium, and culture conditions (Hameg;Gallego;Barreal, 2017;Mitrofanova, 2018;Molkanova et al, 2018).…”

Section: Shoot Multiplicationmentioning

confidence: 99%

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Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

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Actinidia Lindl., commonly known as kiwifruit, is a valuable berry crop. The area of commercial kiwifruit plantations is increasing; the global production of kiwifruit is about 0.62% of the total production of major fruit crops. The use of biotechnological methods, which can significantly accelerate the propagation of quality planting materials, is considered to be relevant for the propagation of this crop. In this study, we optimized the culture medium composition at the micropropagation stage for the effective cultivation of promising cultivars of A. arguta, A. kolomikta, and A. polygama. We investigated the features of Actinidia morphogenesis depending on the genotype, the concentration of 6-Benzylaminopurine (0.5, 0.8, and 1.0 mg L-1), and plant growth regulators (6-Benzylaminopurine, meta-topolin, and 2-isopentenyladenine at a concentration of 0.5 mg L-1) in the media Quoirin and Lepoivre. Actinidia arguta (multiplication rate of 8.0) and A. polygama (6.8) developed faster at the micropropagation stage compared to A. kolomikta (4.9). The studied Actinidia representatives were cultured most effectively on Quoirin and Lepoivre media supplemented with 0.5 mg L-1 meta-topolin, compared to the media containing 0.5 mg L-1 6-Benzylaminopurine and 0.5 mg L-1 2-isopentenyladenine. The use of meta-topolin in the medium contributed to the increase in various morphometric traits, such as the height of microshoots (up to 28% depending on the species), their number (up to 52%), and their multiplication rate (up to 42%). We also recorded a high morphogenic capacity of the investigated species.

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Section: Methodsmentioning

confidence: 99%

Section: Shoot Multiplicationmentioning

confidence: 98%

Section: Shoot Multiplicationmentioning

confidence: 99%

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Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

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“…Обеспечение быстрого размножения эксплантов в течение культивирования в нескольких пассажах достигается снятием апикального доминирования при добавлении в питательную среду оптимальной концентрации цитокинина и правильным подбором питательной среды. Исследованиями, проведенными ранее [16], установлено, что оптимальной питательной средой для культивирования всех косточковых культур являлась среда Кворина-Лепорье в сравнении с Мурасиге-Скуга, Вуди Плант Медиум и Гамборга. Также выявлено, что совместное использование в последнем пассаже пролиферации 6-БАП в концентрации 0,5 мг/л, ГК -0,2 мг/л, ИМК -0,2 мг/л приводило к увеличению выхода пригодных для укоренения микрочеренков.…”

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Optimization of clonal micropropagation of stone fruit crops

Markova,

Somova

2024

Аграрная наука Евро-Северо-Востока

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The aim of the research was to optimize the clonal micropropagation of stone crops through the use of improved techniques. The objects of the research at the stage of introduction into culture in vitro were meristematic apexes, at the stage of micro-propagation itself – micro-shoots, at the rooting stage – rooted micro-shoots, at the adaptation stage – micro-plants. All experiments were carried out according to generally accepted methods using the example of the steppe cherry variety Shchedraya, the domestic plum variety Kazanskaya and the hybrid sweet cherry Fatezh. As a result of the research, it was found that the use of a 10 % chlorhexidine solution for sterilization of the initial plant material of stone crops increased the survival rate of apexes on average by 2.4 %; the combined use of growth regulators 6-benzylaminopurine 0.5 mg/l, gibberellic acid 0.2 mg/l, indo-lil-3-butyric acid (IBA) 0.2 mg/l in a nutrient medium and an experimental diode pulsed phytoirradiator in the last proliferation passage activated the proliferation of micro-shoots, increasing the reproduction coefficient of stone crops in 6 passages by an average of 0.9 pcs/explant; the addition of 1.0 mg/l (IBA ) to the nutrient medium provided an increase in the rooting capacity of micro-shoots of stone crops by an average of 9.6 %. The treatment of micro-plants of stone crops at the stage of adaptation by spraying with an 8 % solution of the extract of the products of the larvae of the large wax moth and the use of an experimental LED pulsed phytoirradiator with a mixed spectrum contributed to an increase in their survival rate by an average of 12.4 %. Compliance with the proposed improved methods of clonal micropropagation of stone crops using growth regulators and an experimental LED pulse phytoirradiator with a mixed spectrum made it possible to increase the yield of standard adapted micro-plants by 2 times. At the same time, the cost of one adapted micro-plant decreased by an average of 11.4 rubles, and the profitability of obtaining improved stone crop material increased by 33.7 % and amounted to 160.7 %.

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Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro

Kulikova¹,

Макаров

Kuznetsova

et al. 2022

Food Processing: Techniques and Technology

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Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots. Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilization time on the viability of honeysuckle explants at the stage of culture introduction in vitro. The effect of the growth regulator Cytodef in the QL nutrient medium on organogenesis was studied at the stage of micropropagation proper, the effect of auxin IBA on plant root formation – at the stage of rooting in vitro. Results and discussion. The greatest viability of honeysuckle explants (80–94%) was registered in the samples affected by Lizoformin 3000 (5%) and silver nitrate (0.2%) as sterilizing agents with a sterilization time of 10 min at the stage of in vitro culture introduction. The biggest quantity (8.8 pcs.) and total length (40.1 cm) of microshoots were observed when the content of cytokinin Cytodef in the culture medium QL was 0.3 mg/L at the stage micropropagation proper. The Boreal Beast cultivar had the largest total length of shoots (29.0 cm). The biggest quantity (5.5 pcs.) and total length (30.8 cm) of roots resulted from 0.5 mg/L of auxin IBA at the stage of rooting in vitro. Coconut substrate produced the highest survival rate (92–99%) at the stage of adaptation to non-sterile conditions in vivo, with the greatest number of leaves (8.1–10.2 pcs.) observed in Canadian cultivars. Conclusion. Cytodef and IBA proved to be effective growth-regulating substances for microplants of Russian and Canadian honeysuckle cultivars in vitro, which makes them promising for berry plantations.

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Improvement of Clonal Micropropagation of Berry Crops

Cited by 3 publications

References 4 publications

Plant growth regulators on the micropropagtion of Actinidia cultivars

Plant growth regulators on the micropropagtion of Actinidia cultivars

Optimization of clonal micropropagation of stone fruit crops

Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro

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